Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   UP12_RS11190 Genome accession   NZ_CP011007
Coordinates   2243017..2244087 (-) Length   356 a.a.
NCBI ID   WP_081106456.1    Uniprot ID   -
Organism   Bacillus pumilus strain SH-B9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2238017..2249087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UP12_RS11140 (UP12_11140) - 2238117..2238689 (-) 573 WP_058014687.1 signal peptidase I -
  UP12_RS11145 (UP12_11145) tapA 2238734..2239261 (-) 528 WP_058014686.1 amyloid fiber anchoring/assembly protein TapA -
  UP12_RS11150 (UP12_11150) - 2239528..2239839 (+) 312 WP_061410249.1 YqzG/YhdC family protein -
  UP12_RS11155 (UP12_11155) - 2239873..2240067 (-) 195 WP_044140973.1 YqzE family protein -
  UP12_RS11160 (UP12_11160) comGG 2240127..2240510 (-) 384 WP_058014684.1 competence type IV pilus minor pilin ComGG -
  UP12_RS11165 (UP12_11165) comGF 2240507..2241007 (-) 501 WP_061408553.1 competence type IV pilus minor pilin ComGF -
  UP12_RS11170 (UP12_11170) comGE 2240946..2241263 (-) 318 WP_058014682.1 competence type IV pilus minor pilin ComGE -
  UP12_RS11175 (UP12_11175) comGD 2241247..2241693 (-) 447 WP_061408555.1 competence type IV pilus minor pilin ComGD -
  UP12_RS11180 (UP12_11180) comGC 2241686..2241979 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  UP12_RS11185 (UP12_11185) comGB 2241996..2243036 (-) 1041 WP_061408557.1 competence type IV pilus assembly protein ComGB -
  UP12_RS11190 (UP12_11190) comGA 2243017..2244087 (-) 1071 WP_081106456.1 competence type IV pilus ATPase ComGA Machinery gene
  UP12_RS11195 - 2244347..2244937 (-) 591 WP_061408560.1 hypothetical protein -
  UP12_RS11200 (UP12_11200) - 2245338..2246210 (-) 873 WP_058014678.1 STAS domain-containing protein -
  UP12_RS11210 (UP12_11210) - 2246434..2246814 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  UP12_RS11215 (UP12_11215) - 2247029..2247271 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  UP12_RS11220 (UP12_11220) - 2247316..2247954 (-) 639 WP_003217463.1 MBL fold metallo-hydrolase -
  UP12_RS19905 (UP12_11225) - 2248112..2248285 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  UP12_RS11225 (UP12_11230) - 2248510..2248824 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40352.75 Da        Isoelectric Point: 9.2484

>NTDB_id=141335 UP12_RS11190 WP_081106456.1 2243017..2244087(-) (comGA) [Bacillus pumilus strain SH-B9]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=141335 UP12_RS11190 WP_081106456.1 2243017..2244087(-) (comGA) [Bacillus pumilus strain SH-B9]
TTGTATGGGATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGTATGAGGGCATCTGATGTGCATATTGTCCC
AAAAGAAAAGGAAGCGTCTGTATCTTTCCGAGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCCCATTTTAAATTTTTATCGTCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCGCTAGCT
GTGATGCTGAGAAGAGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAAAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCTACTCGATTATTGTCGTTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGCAAAACAACGACCCTCTATTCATTGATCCAGTTC
GCAAAAAAGAATTTTAACCGAAATATAATTACACTTGAAGATCCGGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACACTACAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGTC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTCGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment