Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   UP12_RS08650 Genome accession   NZ_CP011007
Coordinates   1766284..1766664 (+) Length   126 a.a.
NCBI ID   WP_244270805.1    Uniprot ID   -
Organism   Bacillus pumilus strain SH-B9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1761284..1771664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UP12_RS08640 (UP12_08650) - 1761774..1764917 (+) 3144 WP_061407935.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  UP12_RS08645 (UP12_08655) - 1765114..1766022 (+) 909 WP_061407938.1 trypsin-like serine protease -
  UP12_RS08650 (UP12_08660) nucA/comI 1766284..1766664 (+) 381 WP_244270805.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  UP12_RS08655 (UP12_08665) - 1766810..1767658 (+) 849 WP_061407940.1 STAS domain-containing protein -
  UP12_RS08660 (UP12_08670) - 1767692..1768234 (-) 543 WP_058015669.1 IseA DL-endopeptidase inhibitor family protein -
  UP12_RS08665 (UP12_08675) - 1768529..1768963 (+) 435 WP_058015668.1 hypothetical protein -
  UP12_RS08670 (UP12_08680) lexA 1769009..1769629 (-) 621 WP_003212311.1 transcriptional repressor LexA -
  UP12_RS08675 (UP12_08685) yneA 1769788..1770099 (+) 312 WP_003211955.1 cell division suppressor protein YneA -
  UP12_RS08680 (UP12_08690) - 1770117..1770764 (+) 648 WP_061407941.1 recombinase family protein -
  UP12_RS08685 (UP12_08695) - 1770834..1771064 (+) 231 WP_003211509.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13767.39 Da        Isoelectric Point: 7.3831

>NTDB_id=141329 UP12_RS08650 WP_244270805.1 1766284..1766664(+) (nucA/comI) [Bacillus pumilus strain SH-B9]
MGTLLGGFGEEHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=141329 UP12_RS08650 WP_244270805.1 1766284..1766664(+) (nucA/comI) [Bacillus pumilus strain SH-B9]
ATGGGCACGCTGTTAGGTGGATTTGGAGAGGAACATGCGGCAAAAGGTGCGGATCGATATGATCATGTTATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCTATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGCATTCCGACTAAGCCTGGATTCGACCGAGATGAGTGGCCG
ATGGCAGTTTGTCTTGAAGGAGGAAAAGGGGCGAGTGTTCAATATGTCAGCCCGTCTGATAATAGGGGAGCAGGCTCATG
GGTCGGACACCAGATAAGTGGGTATCCTGATGGGAAAAGAATTTTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment