Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   UP12_RS05320 Genome accession   NZ_CP011007
Coordinates   1102608..1106306 (+) Length   1232 a.a.
NCBI ID   WP_061407301.1    Uniprot ID   -
Organism   Bacillus pumilus strain SH-B9     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1097608..1111306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UP12_RS05305 (UP12_05305) - 1097904..1098314 (+) 411 WP_058014363.1 DoxX family protein -
  UP12_RS05310 (UP12_05310) - 1098510..1098956 (-) 447 WP_061407295.1 ferritin-like domain-containing protein -
  UP12_RS05315 (UP12_05315) addB 1099121..1102630 (+) 3510 WP_061407298.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  UP12_RS05320 (UP12_05320) addA 1102608..1106306 (+) 3699 WP_061407301.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  UP12_RS05325 (UP12_05325) sbcD 1106333..1107508 (+) 1176 WP_058014359.1 exonuclease subunit SbcD -
  UP12_RS05330 (UP12_05330) - 1107505..1110900 (+) 3396 WP_058014358.1 SMC family ATPase -
  UP12_RS05335 (UP12_05335) - 1110920..1111219 (+) 300 WP_058014357.1 hypothetical protein -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 142214.18 Da        Isoelectric Point: 5.0692

>NTDB_id=141313 UP12_RS05320 WP_061407301.1 1102608..1106306(+) (addA) [Bacillus pumilus strain SH-B9]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIISKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQFLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMASVLEEAGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESQIGHLLESLQQRDLLTDEQIRSINQEGIASFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYRHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAESILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=141313 UP12_RS05320 WP_061407301.1 1102608..1106306(+) (addA) [Bacillus pumilus strain SH-B9]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTACTTGTTGTAACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCCG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGACCGTTACACGTCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCAAGATCTCATCCATCTCCGGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGTCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGTGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTTTATTAGAAAAAGCCACAGATGCACGTAATCAAGCGAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTTGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATTGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATATCAGGATACAAACCTTGTACAAGAAACCATTTTAAAGCTTGTATCTAAAGGAGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGGCTGGCAGAGCCTATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTTCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAACTTACAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAATTGGCTCTGATTCGAACAAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTTATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAGCTAG
AGCGATTTTTCCAATTCCTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGACACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAACAGCGACAGGCGAATCTTCGTGCCCTTTATGA
CAGGGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATCCACAGCAGTAAAGGG
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAACTAGGGTTTGGAAGCAAGTACATCCATCCTGAACTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGAGTCCTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGTAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGGTATCAATCAAAAACATACTTAGATTTCATTGGACCTGCCCTCATCCGTCATCAAGCGATGGCAT
CTGTTTTAGAAGAAGCAGGAGATGTTGTTCTTTCACATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAGGAGCAAGACGAGATTGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCTTATGATGAATCACAGATAGGACACTTGCTCGAATCTTTACAGCAGC
GAGATTTGCTAACAGATGAGCAGATCCGGTCAATTAATCAAGAAGGAATTGCATCTTTCTTCTCTACTTCAATTGGCCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCTTTTAGTATGGTGTTACCTGTTAAAGAGGTATACCGTCATAT
AGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAATCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCATTATATGCAAAAGCTGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTCGATGGGAA
TTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1224)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.181

100

0.664

Multiple sequence alignment