Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   UP12_RS01960 Genome accession   NZ_CP011007
Coordinates   366822..367250 (-) Length   142 a.a.
NCBI ID   WP_058015288.1    Uniprot ID   -
Organism   Bacillus pumilus strain SH-B9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 361822..372250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UP12_RS01930 (UP12_01930) - 361977..362306 (+) 330 WP_058015304.1 YckD family protein -
  UP12_RS01935 (UP12_01935) - 362356..363120 (-) 765 WP_061406708.1 MBL fold metallo-hydrolase -
  UP12_RS01940 (UP12_01940) - 363236..363652 (+) 417 WP_034664465.1 Lrp/AsnC family transcriptional regulator -
  UP12_RS01945 (UP12_01945) - 363682..364488 (+) 807 WP_058015285.1 AAC(3) family N-acetyltransferase -
  UP12_RS01950 (UP12_01950) - 364490..364876 (-) 387 WP_058015286.1 competence protein ComJ -
  UP12_RS01955 (UP12_01955) - 364997..366694 (-) 1698 WP_061406710.1 methyl-accepting chemotaxis protein -
  UP12_RS01960 (UP12_01960) nucA/comI 366822..367250 (-) 429 WP_058015288.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  UP12_RS01970 (UP12_01970) - 367618..369693 (+) 2076 WP_061406712.1 hydantoinase/oxoprolinase family protein -
  UP12_RS01975 (UP12_01975) - 369683..371668 (+) 1986 WP_058015290.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15893.83 Da        Isoelectric Point: 5.8519

>NTDB_id=141305 UP12_RS01960 WP_058015288.1 366822..367250(-) (nucA/comI) [Bacillus pumilus strain SH-B9]
MKLLKIILLLLLIIVGVATGYIQLEHGKQETTNSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSVCTIDRKHSDEQREQS
LHGIPTKRGYDRDEWPMAMCKEGGTGASVKYISPSDNRGAGSWVGHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=141305 UP12_RS01960 WP_058015288.1 366822..367250(-) (nucA/comI) [Bacillus pumilus strain SH-B9]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTACTGATCATCGTTGGTGTAGCAACAGGCTATATTCAGCTGGAGCACGG
TAAACAAGAGACGACGAATTCATCTTATGACAAAACGATTCACTTCCCATCAGATCGTTACCCCGAAACCGCGAAGCACA
TAGAAGAGGCAATTGATGAAGGCCATTCATCCGTATGTACAATTGACCGTAAACATAGCGATGAACAAAGAGAACAATCA
CTTCATGGCATCCCAACAAAGCGCGGATATGATCGGGACGAATGGCCGATGGCGATGTGTAAGGAAGGAGGAACAGGAGC
TTCAGTCAAATACATTAGTCCTTCAGATAATAGAGGAGCAGGCTCATGGGTCGGTCATCAATTATCAGATGATCCTGACG
GCACAAGAATACAATTTATCATAGATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

74.528

74.648

0.556

Multiple sequence alignment