Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N805_RS26340 Genome accession   NZ_CP010979
Coordinates   5967598..5968968 (+) Length   456 a.a.
NCBI ID   WP_019472669.1    Uniprot ID   A0AAU8S4T9
Organism   Pseudomonas putida S13.1.2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5962598..5973968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N805_RS26310 (N805_26315) cydB 5962681..5963688 (+) 1008 WP_019473084.1 cytochrome d ubiquinol oxidase subunit II -
  N805_RS26315 (N805_26320) - 5963698..5963853 (+) 156 WP_016489104.1 DUF2474 domain-containing protein -
  N805_RS26320 (N805_26325) - 5963968..5965092 (-) 1125 WP_019472665.1 methyltransferase -
  N805_RS26325 (N805_26330) - 5965189..5965896 (-) 708 WP_019472666.1 autoinducer binding domain-containing protein -
  N805_RS26330 (N805_26335) - 5966108..5966884 (+) 777 WP_026034600.1 ferredoxin--NADP reductase -
  N805_RS26335 (N805_26340) mscL 5967002..5967421 (-) 420 WP_019472668.1 large-conductance mechanosensitive channel protein MscL -
  N805_RS26340 (N805_26345) radA 5967598..5968968 (+) 1371 WP_019472669.1 DNA repair protein RadA Machinery gene
  N805_RS26345 (N805_26350) - 5969144..5970661 (-) 1518 WP_019472670.1 nucleobase:cation symporter-2 family protein -
  N805_RS26350 (N805_26355) - 5970925..5971290 (-) 366 WP_019472671.1 PilZ domain-containing protein -
  N805_RS26355 (N805_26360) - 5971468..5973534 (+) 2067 WP_019472672.1 carbon starvation CstA family protein -
  N805_RS26360 (N805_26365) - 5973552..5973749 (+) 198 WP_003251633.1 YbdD/YjiX family protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48757.18 Da        Isoelectric Point: 6.8985

>NTDB_id=141086 N805_RS26340 WP_019472669.1 5967598..5968968(+) (radA) [Pseudomonas putida S13.1.2]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTE
LDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETII
ATARVEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYF
EGESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHL
ANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLS
GEVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKESPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=141086 N805_RS26340 WP_019472669.1 5967598..5968968(+) (radA) [Pseudomonas putida S13.1.2]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACCGAGTGTGGCGCGACCTTTCCCAAATGGGCCGGCCAGTGCGGCGAATG
CGGGGCCTGGAACACGCTGGTCGAAACCATGATCGAGAGCGGCGGTGCCGCTGCGCCCAGTAGCGGCCGCGCCGGCTGGA
CCGGGCAGCAAGCGCAGATCAAGACCCTGGCCGAAGTCAGTGTCGAGGAAATCCCGCGCTTTACCACCAGCAGCACCGAA
CTGGACCGTGTGTTGGGCGGCGGCCTGGTGGATGGCTCGGTGGTGCTGATTGGTGGCGACCCTGGCATCGGCAAGTCGAC
CATCCTGCTGCAGACCTTGTGCAACATCGCCGTGGGCATGCCGGCGCTGTATGTCACCGGGGAGGAGTCGCAGCAGCAGG
TGGCCATGCGCTCGCGGCGCCTGGGCTTGCCCCAGGACCAGCTGAAGGTGATGACCGAGACCTGCATCGAAACCATCATT
GCCACGGCGCGGGTCGAGAAACCGCGGGTAATGGTGATCGACTCGATCCAGACCATCTTCACCGAGCAGTTGCAGTCGGC
ACCTGGCGGCGTGGCCCAGGTGCGCGAGAGCACGGCGCTGCTGGTGCGTTACGCCAAGCAGAGCGGCACGGCGATCTTCC
TGGTCGGCCACGTGACCAAGGAGGGTTCGCTGGCCGGGCCACGGGTACTGGAGCACATGGTCGATACCGTGCTGTATTTC
GAAGGTGAGTCCGACGGCCGCCTGCGGCTGTTGCGGGCGGTGAAGAACCGCTTTGGCGCGGTCAATGAACTGGGCGTGTT
CGGCATGACCGACCGGGGCCTGAAAGAGGTATCCAACCCGTCGGCGATCTTCCTTAACCGCACCCAGGAAGAAGTGCCGG
GCAGTGTGGTGATGGCCACCTGGGAGGGCACCCGGCCAATGCTGGTCGAGGTACAAGCACTGGTCGACGACAGCCACCTG
GCCAACCCGCGCCGGGTAACCCTGGGCCTGGACCAGAACCGCTTGGCCATGTTGCTGGCGGTGCTGCACCGCCACGGCGG
TATTCCTACCCACGATCAGGACGTGTTCCTCAACGTGGTGGGCGGGGTCAAGGTGCTGGAAACGGCTTCGGACCTGGCGT
TGCTGGCGGCAGTGATGTCCAGCTTGCGTAACCGGCCACTGGCCCATGGGCTGCTGGTGTTTGGCGAGATCGGCCTGTCG
GGCGAGGTGCGGCCCGTGCCTAGCGGGCAGGAGCGTTTGAAGGAGGCGGCCAAGCATGGCTTCAAGCGCGCCATCGTGCC
CAAGGGCAATGCGCCGAAAGAGTCGCCGGCGGGGCTGCAGGTTATTGCCGTCACCCGCCTGGAACAGGCCCTGGATGCAT
TGTTCGAGTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(355-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.487
  radA Streptococcus mitis NCTC 12261

45.87

100

0.463
  radA Streptococcus pneumoniae D39

46.053

100

0.461
  radA Streptococcus pneumoniae TIGR4

46.053

100

0.461
  radA Streptococcus mitis SK321

46.053

100

0.461
  radA Streptococcus pneumoniae R6

46.053

100

0.461
  radA Streptococcus pneumoniae Rx1

46.053

100

0.461

Multiple sequence alignment