Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   UC35_RS11400 Genome accession   NZ_CP010951
Coordinates   2359259..2360797 (+) Length   512 a.a.
NCBI ID   WP_061499514.1    Uniprot ID   A0A127JTZ8
Organism   Ramlibacter tataouinensis strain 5-10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2354259..2365797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UC35_RS11375 (UC35_11390) argS 2354759..2356432 (+) 1674 WP_061499500.1 arginine--tRNA ligase -
  UC35_RS11380 (UC35_11395) - 2356449..2357078 (+) 630 WP_061499503.1 SPOR domain-containing protein -
  UC35_RS11385 (UC35_11400) - 2357100..2357741 (+) 642 WP_061499506.1 thiol:disulfide interchange protein DsbA/DsbL -
  UC35_RS11390 (UC35_11405) lptA 2357854..2358462 (+) 609 WP_061499508.1 lipopolysaccharide transport periplasmic protein LptA -
  UC35_RS11395 (UC35_11410) lptB 2358525..2359247 (+) 723 WP_082793559.1 LPS export ABC transporter ATP-binding protein -
  UC35_RS11400 (UC35_11415) rpoN/rpoN1 2359259..2360797 (+) 1539 WP_061499514.1 RNA polymerase factor sigma-54 Machinery gene
  UC35_RS11405 (UC35_11420) - 2360812..2362038 (+) 1227 WP_061499517.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  UC35_RS11410 (UC35_11425) - 2362019..2362435 (+) 417 WP_082793055.1 putative toxin-antitoxin system toxin component, PIN family -
  UC35_RS11415 (UC35_11430) - 2362432..2363331 (-) 900 WP_061499521.1 alpha/beta fold hydrolase -
  UC35_RS23505 (UC35_11435) - 2363328..2363816 (-) 489 WP_227820528.1 CinA family protein -
  UC35_RS23510 (UC35_11440) - 2363813..2364343 (-) 531 WP_061503799.1 phosphatidylglycerophosphatase A -
  UC35_RS11430 (UC35_11445) - 2364340..2365257 (-) 918 WP_061499524.1 LysR family transcriptional regulator -
  UC35_RS11435 (UC35_11450) - 2365374..2365568 (+) 195 WP_061499527.1 hypothetical protein -

Sequence

Protein


Download         Length: 512 a.a.        Molecular weight: 56591.82 Da        Isoelectric Point: 4.8589

>NTDB_id=140858 UC35_RS11400 WP_061499514.1 2359259..2360797(+) (rpoN/rpoN1) [Ramlibacter tataouinensis strain 5-10]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDDNPFLELNEDELPREEFGLSHSDVPAADDDAGEFHVNG
AADSQPAVDSEIAGEPEAPSWEGDGSTELAPDDNEWGGDATPRQANGSADDETDATELARSHESLPAHLHRQALALRLGE
EDRCALRFLIESLNDDGYLEEPLEALAESLAPDDLEQREELVHRFTVALRLLQHLDPPGVGARTLGECLTLQLQARQAAG
DASEACAAALRICAQPMELLARRDIKRLAQLCGETDAQVRAAIALVARLDPKPGRRFVDVERNVIVPDVLVTKVGRGAQA
RFRVQLNPDVMPRLRVHDVYAGALKAHKGEGHQALQQRLQEARWFIKNIQQRFDTILRVSNAIVERQKNFFMHGELAMRP
LVLREIADELGLHESTISRVTTAKYMSTPFGTFELKYFFGSALGTETGGNASSTAVRALIKQFIASESPQKPLSDNQISE
MLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1539 bp        

>NTDB_id=140858 UC35_RS11400 WP_061499514.1 2359259..2360797(+) (rpoN/rpoN1) [Ramlibacter tataouinensis strain 5-10]
ATGAAACAGGGCTTGTCCCTTCGCGTGTCGCAGCACCTGGCCTTGACGCCGCAGCTGCAACAGTCCATCCGCCTGCTGCA
GCTGTCCACCCTGGAGCTGTCGCAGGAAGTCGAGCAGATGCTCGACGACAACCCTTTCCTGGAGTTGAACGAGGACGAGC
TGCCGCGCGAGGAGTTCGGCCTGTCGCACAGCGACGTGCCGGCGGCGGACGACGACGCCGGCGAATTCCATGTCAATGGC
GCGGCCGACAGCCAGCCGGCCGTGGACAGTGAGATTGCCGGCGAGCCGGAGGCGCCCAGCTGGGAGGGCGACGGCAGCAC
CGAGCTGGCGCCGGACGACAACGAGTGGGGCGGCGACGCGACGCCGCGCCAGGCCAACGGGAGCGCCGACGACGAGACCG
ACGCCACCGAGCTGGCGCGCAGCCACGAATCGCTGCCGGCGCACCTGCACCGCCAGGCGCTGGCGCTGCGCCTGGGCGAG
GAAGACCGCTGCGCGCTGCGCTTCCTGATCGAATCGCTGAACGACGACGGCTACCTGGAGGAGCCGCTCGAGGCGCTGGC
CGAGAGCCTGGCGCCCGACGACCTGGAGCAGCGCGAAGAGCTGGTCCACCGCTTCACCGTGGCCCTGCGCCTGCTGCAGC
ACCTGGACCCGCCGGGGGTGGGCGCGCGCACGCTGGGCGAGTGCCTCACGCTGCAGCTGCAGGCCAGGCAGGCGGCCGGC
GACGCGTCCGAGGCCTGCGCCGCGGCGCTGCGCATCTGCGCGCAGCCGATGGAGCTGCTGGCGCGCCGCGACATCAAGCG
CCTGGCCCAGCTGTGCGGCGAGACGGACGCGCAGGTTCGCGCCGCCATCGCCCTGGTCGCACGGCTCGATCCCAAGCCGG
GGCGGCGCTTCGTCGACGTGGAGCGCAACGTGATCGTGCCCGACGTGCTCGTCACCAAGGTGGGACGCGGGGCGCAGGCG
CGCTTTCGCGTGCAGCTCAACCCCGACGTGATGCCGCGCCTGCGCGTGCACGACGTCTATGCCGGCGCGCTCAAGGCGCA
CAAGGGCGAGGGCCACCAGGCGCTGCAGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCG
ACACCATCCTGCGCGTGTCCAATGCCATCGTGGAGCGCCAGAAGAACTTCTTCATGCACGGCGAGCTGGCGATGCGGCCA
TTGGTGCTGCGCGAGATCGCCGACGAGCTGGGCCTGCACGAGTCGACCATCAGCCGCGTCACCACGGCCAAGTACATGTC
CACGCCCTTCGGCACCTTCGAGCTGAAGTACTTCTTCGGCTCGGCCCTGGGGACCGAGACCGGGGGCAACGCCTCGAGCA
CGGCGGTGCGGGCGCTGATCAAGCAGTTCATCGCCTCCGAGAGCCCGCAAAAGCCCCTGTCGGACAACCAGATCTCCGAG
ATGCTGAAGGAGCAGGGCATCGAATGCGCGCGCCGCACCGTGGCCAAGTACCGCGAGGCGCTGCGCATCGCCCCCGCCAA
CCTGCGCAAGGCGCTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-340)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(354-510)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127JTZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.731

100

0.525

Multiple sequence alignment