Detailed information NTG0140585

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: vpachn25_RS14060
• Genome accession: NZ_CP010883
• Coordinates: 3060700..3061716 (-)
• Length: 338 a.a.
• NCBI ID: WP_017449463.1
• Uniprot ID: A0A7Y0SFN2
• Organism: Vibrio parahaemolyticus strain CHN25
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3055700..3066716

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
vpachn25_RS14035 (VpaChn25_2673)	aroK	3056560..3057078 (-)	519	WP_005381319.1	shikimate kinase AroK	-
vpachn25_RS14040 (VpaChn25_2674)	pilQ	3057267..3059015 (-)	1749	WP_005488227.1	type IV pilus secretin PilQ	Machinery gene
vpachn25_RS14045 (VpaChn25_2675)	pilP	3059047..3059562 (-)	516	WP_005454781.1	pilus assembly protein PilP	Machinery gene
vpachn25_RS14050 (VpaChn25_2676)	pilO	3059552..3060145 (-)	594	WP_005496571.1	type 4a pilus biogenesis protein PilO	Machinery gene
vpachn25_RS14055 (VpaChn25_2677)	pilN	3060138..3060716 (-)	579	WP_015297396.1	PilN domain-containing protein	Machinery gene
vpachn25_RS14060 (VpaChn25_2678)	pilM	3060700..3061716 (-)	1017	WP_017449463.1	type IV pilus biogenesis protein PilM	Machinery gene
vpachn25_RS14065 (VpaChn25_2679)	-	3061859..3064429 (+)	2571	WP_005496567.1	penicillin-binding protein 1A	-
vpachn25_RS14070 (VpaChn25_2680)	oxyR	3064485..3065393 (-)	909	WP_005465160.1	DNA-binding transcriptional regulator OxyR	-
vpachn25_RS14075 (VpaChn25_2681)	-	3065564..3066292 (+)	729	WP_015297397.1	redoxin family protein	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37748.26 Da
• Isoelectric Point: 6.8322

>NTDB_id=140585 vpachn25_RS14060 WP_017449463.1 3060700..3061716(-) (pilM) [Vibrio parahaemolyticus strain CHN25]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide

• Length: 1017 bp

>NTDB_id=140585 vpachn25_RS14060 WP_017449463.1 3060700..3061716(-) (pilM) [Vibrio parahaemolyticus strain CHN25]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCAGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGCTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-326)

3D structure

• AlphaFold DB: A0A7Y0SFN2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	84.911	100	0.849
pilM	Vibrio cholerae strain A1552	48.368	99.704	0.482