Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ASZ83_RS01545 Genome accession   NZ_CP013315
Coordinates   307003..307587 (+) Length   194 a.a.
NCBI ID   WP_000902745.1    Uniprot ID   -
Organism   Vibrio cholerae strain M2140     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 302003..312587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASZ83_RS01530 (ASZ83_00297) oxyR 302410..303303 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  ASZ83_RS01535 (ASZ83_00298) - 303377..305854 (-) 2478 WP_000668733.1 penicillin-binding protein 1A -
  ASZ83_RS01540 (ASZ83_00299) pilM 305997..307013 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  ASZ83_RS01545 (ASZ83_00300) pilN 307003..307587 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  ASZ83_RS01550 (ASZ83_00301) pilO 307580..308170 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  ASZ83_RS01555 (ASZ83_00302) pilP 308160..308678 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  ASZ83_RS01560 (ASZ83_00303) pilQ 308704..310419 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  ASZ83_RS01565 (ASZ83_00304) aroK 310606..311130 (+) 525 WP_000818616.1 shikimate kinase AroK -
  ASZ83_RS01570 (ASZ83_00305) aroB 311158..312243 (+) 1086 WP_000439805.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 21964.42 Da        Isoelectric Point: 9.9503

>NTDB_id=140535 ASZ83_RS01545 WP_000902745.1 307003..307587(+) (pilN) [Vibrio cholerae strain M2140]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQAGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=140535 ASZ83_RS01545 WP_000902745.1 307003..307587(+) (pilN) [Vibrio cholerae strain M2140]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCAGCAGGCGAATACTTGGGCGGACAAATGGCTTTGCAACAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGCGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

100

100

1
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552