Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HR49_RS00735 Genome accession   NZ_CP010820
Coordinates   164745..166121 (+) Length   458 a.a.
NCBI ID   WP_036121649.1    Uniprot ID   -
Organism   Lysinibacillus fusiformis strain RB-21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 159745..171121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR49_RS00715 (HR49_00715) - 160087..160557 (+) 471 WP_031416734.1 CtsR family transcriptional regulator -
  HR49_RS00720 (HR49_00720) - 160571..161113 (+) 543 WP_025116467.1 UvrB/UvrC motif-containing protein -
  HR49_RS00725 (HR49_00725) - 161110..162219 (+) 1110 WP_036121652.1 protein arginine kinase -
  HR49_RS00730 (HR49_00730) - 162209..164653 (+) 2445 WP_008180611.1 ATP-dependent Clp protease ATP-binding subunit -
  HR49_RS00735 (HR49_00735) radA 164745..166121 (+) 1377 WP_036121649.1 DNA repair protein RadA Machinery gene
  HR49_RS00740 (HR49_00740) - 166338..167423 (+) 1086 WP_036121647.1 PIN/TRAM domain-containing protein -
  HR49_RS00745 (HR49_00745) ispD 167435..168130 (+) 696 WP_036121646.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HR49_RS00750 (HR49_00750) ispF 168516..168992 (+) 477 WP_036121644.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  HR49_RS00755 (HR49_00755) gltX 169081..170541 (+) 1461 WP_036121641.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50183.01 Da        Isoelectric Point: 7.3825

>NTDB_id=140058 HR49_RS00735 WP_036121649.1 164745..166121(+) (radA) [Lysinibacillus fusiformis strain RB-21]
MAKKKTKFVCSGCGYESAKWMGRCPGCGEWNKMVEEVEVVAKGPRGAFQHSATVTQKAIPIIQVEASEESRVATEMGELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSALLSNKGHRVLYISGEESIRQTKLRAERLGVISQELYIYSETNLEFLNQ
TIEEVQPKFVIVDSIQTVFHPEVTSAPGSVSQVRECTAELMRIAKTKGIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTIVASMEGTRPILVEIQSLVTPTSFN
YPKRMATGVDQNRVQLLMAVLEKRMGLMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVRATDCFVGEVGLTG
EVRRVSRIEQRVIEAAKLGFKRVFIPTSNIGGWDFPQGIEIIGVETIKDALNACFREL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=140058 HR49_RS00735 WP_036121649.1 164745..166121(+) (radA) [Lysinibacillus fusiformis strain RB-21]
TTGGCAAAGAAAAAAACGAAATTTGTATGTTCAGGCTGTGGCTATGAATCAGCAAAATGGATGGGAAGATGTCCAGGGTG
TGGGGAATGGAATAAGATGGTGGAGGAAGTAGAAGTGGTTGCGAAAGGACCACGTGGGGCTTTCCAGCATTCTGCCACTG
TTACTCAAAAGGCAATCCCTATCATTCAGGTGGAAGCTAGTGAAGAGTCCCGTGTAGCGACAGAAATGGGCGAGCTGAAC
CGTGTATTAGGCGGTGGTATTGTGCCTGGCTCTCTAGTATTAATAGGCGGTGATCCAGGGATTGGGAAATCCACTTTATT
ACTACAAGTTTCAGCCTTGCTTTCTAATAAAGGTCATCGTGTGCTGTATATTTCTGGTGAGGAATCTATCCGTCAAACGA
AGCTACGTGCGGAGCGTTTGGGGGTAATTTCGCAAGAGCTGTATATTTATTCTGAAACAAATCTTGAGTTTTTGAATCAA
ACGATTGAGGAAGTACAGCCGAAATTTGTCATTGTCGATTCCATACAAACCGTCTTTCATCCAGAGGTAACAAGTGCGCC
AGGTAGTGTCTCACAGGTCCGTGAGTGTACAGCTGAGCTTATGCGAATTGCTAAGACAAAAGGAATTGCGATCTTCTTAG
TTGGGCATGTTACGAAAGAGGGGCAAATAGCAGGGCCTCGTATTTTAGAGCATATGGTGGATACAGTCCTGTATTTTGAA
GGGGAAAGACATCATAATCATCGTATTTTACGAAGTCAAAAGAACCGCTTCGGTTCTACAAATGAAATTGCTATATTTGA
AATGCTACAGGGTGGGCTAAAAGAGGTTTTGAACCCATCTGAGCTGTTTTTACAGGAGCGTTCACAAGGAGCTGCGGGCT
CCACGATTGTTGCCTCAATGGAGGGAACAAGGCCAATACTCGTTGAAATCCAGTCACTCGTAACGCCTACCAGCTTTAAT
TACCCTAAGCGGATGGCAACAGGGGTTGATCAAAATAGAGTGCAATTACTGATGGCCGTACTTGAAAAGCGTATGGGTCT
CATGCTACAAGCGCAGGATGCATATATTAAAGTTGCTGGTGGTGTAAAATTGGACGAACCTGCCATCGATTTAGCTGTTC
TGACAAGCATCGTTTCTAGCTTTAAGGATCAGGCTGTGCGAGCAACGGATTGCTTTGTTGGTGAGGTAGGTCTGACAGGA
GAGGTGCGTCGAGTATCCCGTATTGAGCAACGTGTCATTGAAGCAGCGAAGCTTGGTTTTAAAAGAGTATTCATACCTAC
TTCCAATATAGGTGGATGGGATTTTCCACAAGGGATTGAAATTATCGGGGTAGAAACGATAAAGGATGCATTAAATGCCT
GCTTTAGAGAGTTATAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(350-432)

AAA domain (AAA_25)

(66-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.705

99.127

0.701
  radA Streptococcus pneumoniae Rx1

63.158

99.563

0.629
  radA Streptococcus pneumoniae D39

63.158

99.563

0.629
  radA Streptococcus pneumoniae R6

63.158

99.563

0.629
  radA Streptococcus pneumoniae TIGR4

63.158

99.563

0.629
  radA Streptococcus mitis NCTC 12261

63.158

99.563

0.629
  radA Streptococcus mitis SK321

63.158

99.563

0.629

Multiple sequence alignment