Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC22_RS14445 Genome accession   NZ_CP013248
Coordinates   2958861..2960585 (-) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain FORC_022     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2953861..2965585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC22_RS14410 (FORC22_2730) - 2954176..2954889 (-) 714 WP_025525977.1 PilW family protein -
  FORC22_RS14415 (FORC22_2731) - 2954889..2955488 (-) 600 WP_071652422.1 type II secretion system protein -
  FORC22_RS14420 (FORC22_2732) - 2955485..2955964 (-) 480 WP_025525975.1 type II secretion system protein -
  FORC22_RS14425 (FORC22_2733) - 2956080..2956313 (-) 234 WP_005388453.1 type II secretion system protein -
  FORC22_RS14430 (FORC22_2734) - 2956378..2956869 (-) 492 WP_021484734.1 type II secretion system protein -
  FORC22_RS14435 (FORC22_2735) - 2957164..2957625 (-) 462 WP_029832534.1 hypothetical protein -
  FORC22_RS14440 (FORC22_2736) - 2957629..2958852 (-) 1224 WP_021484749.1 type II secretion system F family protein -
  FORC22_RS14445 (FORC22_2737) pilB 2958861..2960585 (-) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  FORC22_RS14450 (FORC22_2738) - 2960582..2961658 (-) 1077 WP_195183112.1 tetratricopeptide repeat protein -
  FORC22_RS14455 (FORC22_2739) - 2961655..2962500 (-) 846 WP_025579906.1 ExeA family protein -
  FORC22_RS14460 (FORC22_2740) mshL 2962544..2964187 (-) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  FORC22_RS14465 (FORC22_2741) - 2964208..2964537 (-) 330 WP_071652424.1 MSHA biogenesis protein MshK -
  FORC22_RS14470 (FORC22_2742) gspM 2964530..2965180 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=139917 FORC22_RS14445 WP_005456306.1 2958861..2960585(-) (pilB) [Vibrio parahaemolyticus strain FORC_022]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=139917 FORC22_RS14445 WP_005456306.1 2958861..2960585(-) (pilB) [Vibrio parahaemolyticus strain FORC_022]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTCGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGATCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTTGGATTACCGTTGATCGATCTTGGACGTGCTCCAGTGGATGCAGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTGGCTCGTAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATAGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACTAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCAGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCTGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGACGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGCCAATCGATTGATAT
TCGTATGTCGACGTTACCAACTCAGTACGGTGAATCGGTTGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACCGGCCCTACGGGATCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACCTGACATTTT
CACGCGTTCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGTGACCAAGAGACGGTAGAG
ATTGGCTTGCGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGTTGCACACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTGGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCAGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCAGGTTGTCAAAACTGTAATTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361