Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   TH63_RS18045 Genome accession   NZ_CP010777
Coordinates   4436778..4438157 (-) Length   459 a.a.
NCBI ID   WP_048922962.1    Uniprot ID   A0A0H4VPG4
Organism   Rufibacter radiotolerans strain DG31D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4431778..4443157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TH63_RS18020 (TH63_18030) - 4432108..4432296 (+) 189 WP_048922173.1 hypothetical protein -
  TH63_RS18025 (TH63_18035) - 4432548..4432886 (+) 339 WP_048922174.1 hypothetical protein -
  TH63_RS18030 (TH63_18040) - 4432925..4433932 (-) 1008 WP_076606536.1 mechanosensitive ion channel family protein -
  TH63_RS18035 (TH63_18045) - 4434094..4435119 (-) 1026 WP_048922175.1 SPASM domain-containing protein -
  TH63_RS18040 (TH63_18050) - 4435244..4436686 (-) 1443 WP_048922176.1 peroxiredoxin -
  TH63_RS18045 (TH63_18055) radA 4436778..4438157 (-) 1380 WP_048922962.1 DNA repair protein RadA Machinery gene
  TH63_RS18050 (TH63_18060) - 4438384..4438830 (+) 447 WP_197088592.1 hypothetical protein -
  TH63_RS18055 (TH63_18065) - 4438921..4440333 (+) 1413 WP_048922178.1 ribosomal RNA small subunit methyltransferase F -
  TH63_RS18060 (TH63_18070) - 4440593..4441051 (+) 459 WP_197088593.1 hypothetical protein -
  TH63_RS18065 (TH63_18075) - 4441193..4442380 (-) 1188 WP_048922180.1 class I SAM-dependent rRNA methyltransferase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50088.40 Da        Isoelectric Point: 6.1253

>NTDB_id=139660 TH63_RS18045 WP_048922962.1 4436778..4438157(-) (radA) [Rufibacter radiotolerans strain DG31D]
MAKIKTAYFCQSCGAQSAKWIGKCPACGEWNTYVEEVLLREDVTPTSAWKVGTSSQQVASKPKAINDITYSEQARIETTD
AELNRVLGGGIVPGSMVLIGGEPGIGKSTLMLQIALSLRAQRVLYISGEESEQQIKMRAERLGAQHPNCFILTETGTQNI
FKQIEQLQPDVLIVDSIQTLHSSFIESGAGSVAQVRECTAELLKFAKESGTPVFLIGHITKEGSLAGPKILEHMVDTVLT
FEGDRHMTYRILRTTKNRFGSTSELGIYEMLGTGLREVSNPSEILISQREESFSGITIGGTMEGNRPLLIEVQSLVTPAT
YGTPQRSSTGFDVKRLNMLLAVLEKRGGFKMGTQDVFLNIAGGLKVEDPALDLAICAAMLSSFEDIAIPHTSCFAAEVGL
GGEVRAVHRIEQRISEAEKLGFQDIYISKYNKKGVDLSRFNIRIHAASRLEEVFSGLFG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=139660 TH63_RS18045 WP_048922962.1 4436778..4438157(-) (radA) [Rufibacter radiotolerans strain DG31D]
ATGGCTAAAATCAAAACAGCATATTTCTGCCAGAGCTGCGGCGCCCAATCGGCTAAATGGATAGGCAAGTGCCCCGCCTG
CGGCGAATGGAATACCTACGTAGAGGAAGTTCTGTTGCGCGAGGACGTCACTCCTACCAGCGCCTGGAAAGTGGGCACCA
GCAGCCAGCAGGTGGCCAGCAAACCCAAGGCTATCAATGATATTACCTACTCAGAGCAGGCCCGCATTGAAACCACTGAC
GCCGAACTGAACCGCGTGCTGGGGGGCGGCATCGTGCCGGGCTCCATGGTACTGATCGGCGGCGAGCCGGGCATTGGCAA
GTCTACGCTCATGCTGCAGATTGCCCTGAGTCTGCGCGCCCAAAGGGTGCTTTATATCTCTGGCGAGGAAAGCGAGCAGC
AGATCAAGATGCGCGCCGAACGCCTGGGCGCCCAACACCCCAACTGCTTTATCTTAACCGAGACCGGCACCCAGAACATC
TTCAAGCAGATTGAACAGCTCCAGCCAGATGTCTTGATCGTGGACTCCATTCAGACGCTGCATTCTTCCTTTATTGAAAG
CGGGGCGGGTAGTGTGGCGCAGGTGCGCGAGTGTACCGCCGAATTGCTCAAGTTCGCAAAGGAGAGCGGCACGCCGGTGT
TTTTGATCGGGCATATCACCAAAGAAGGCAGCCTGGCCGGGCCTAAGATTCTGGAGCACATGGTAGACACCGTGTTGACT
TTTGAAGGCGACCGCCACATGACCTACCGCATTCTGCGCACCACCAAAAACCGCTTCGGGAGCACGTCTGAACTAGGTAT
CTATGAAATGCTGGGCACTGGCCTGCGGGAAGTAAGCAACCCATCTGAAATCCTGATTTCGCAGCGCGAAGAGTCCTTCA
GTGGCATTACCATTGGCGGCACCATGGAAGGCAACCGACCCTTATTAATTGAGGTGCAGAGCCTGGTCACGCCCGCCACC
TACGGTACGCCGCAACGCAGCTCCACGGGGTTTGACGTGAAACGCCTGAACATGCTGCTGGCCGTGCTGGAGAAACGCGG
GGGCTTTAAAATGGGTACCCAGGACGTGTTCCTGAACATTGCCGGCGGCCTAAAGGTAGAGGACCCCGCCCTGGATCTGG
CCATCTGCGCCGCCATGCTGTCCTCGTTTGAGGACATCGCTATACCGCACACCTCCTGCTTTGCCGCTGAGGTGGGCCTG
GGGGGCGAGGTACGGGCGGTACACCGCATAGAACAACGCATCTCAGAGGCCGAAAAGCTCGGTTTCCAGGATATTTATAT
TTCAAAGTACAACAAGAAAGGGGTGGACCTGAGCCGGTTTAACATCAGAATTCATGCGGCCAGCCGTTTAGAGGAGGTGT
TCAGTGGGTTGTTTGGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(74-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(346-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H4VPG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

53.44

94.989

0.508
  radA Streptococcus pneumoniae Rx1

49.348

100

0.495
  radA Streptococcus pneumoniae D39

49.348

100

0.495
  radA Streptococcus pneumoniae R6

49.348

100

0.495
  radA Streptococcus pneumoniae TIGR4

49.348

100

0.495
  radA Streptococcus mitis NCTC 12261

49.13

100

0.492
  radA Streptococcus mitis SK321

49.13

100

0.492

Multiple sequence alignment