Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   AS852_RS08735 Genome accession   NZ_CP013218
Coordinates   1726900..1729173 (-) Length   757 a.a.
NCBI ID   WP_071938170.1    Uniprot ID   -
Organism   Staphylococcus aureus strain LA-MRSA ST398 isolate E154     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1721900..1734173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS852_RS08715 (AS852_08400) - 1722407..1723282 (-) 876 WP_000717800.1 N-acetylmuramoyl-L-alanine amidase -
  AS852_RS08720 (AS852_08405) dtd 1723279..1723731 (-) 453 WP_000869979.1 D-aminoacyl-tRNA deacylase -
  AS852_RS08725 (AS852_08410) - 1723743..1725932 (-) 2190 WP_001058583.1 RelA/SpoT family protein -
  AS852_RS08730 (AS852_08415) - 1726360..1726878 (-) 519 WP_000364542.1 adenine phosphoribosyltransferase -
  AS852_RS08735 (AS852_08420) recJ 1726900..1729173 (-) 2274 WP_071938170.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  AS852_RS08740 (AS852_08425) secDF 1729376..1731655 (-) 2280 WP_000749801.1 protein translocase subunit SecDF -
  AS852_RS08745 (AS852_08430) yajC 1731930..1732190 (-) 261 WP_001160682.1 preprotein translocase subunit YajC -
  AS852_RS08750 (AS852_08435) tgt 1732209..1733348 (-) 1140 WP_001112045.1 tRNA guanosine(34) transglycosylase Tgt -

Sequence

Protein


Download         Length: 757 a.a.        Molecular weight: 85600.84 Da        Isoelectric Point: 4.8093

>NTDB_id=139607 AS852_RS08735 WP_071938170.1 1726900..1729173(-) (recJ) [Staphylococcus aureus strain LA-MRSA ST398 isolate E154]
MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDVNHDALQLSDMTKAIDRIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSILPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERK
DIVATITEEAMAMAETKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEIL
SAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKPVDVLLTENDITIKNIRDMNRLRPFGTDFS
RPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMN
EQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPMSMEDLSNSLQQLQFSQLYIVL
QHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCEHLSVKPDTLKFMLKVFLDLKFVTQEDSLIRINQQPDKRS
IDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2274 bp        

>NTDB_id=139607 AS852_RS08735 WP_071938170.1 1726900..1729173(-) (recJ) [Staphylococcus aureus strain LA-MRSA ST398 isolate E154]
ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAGCAATTGAATCCATCATTTCAGACACAG
ATGTCAATCATGATGCATTGCAATTGAGCGATATGACTAAGGCTATTGATAGAATTAAAAGAGCGATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTATATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGTGTTGCGTACAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCATTAGTTGCGATAGGTACCATTGCGGACTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GCGAATTGTTGATGACTGACGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAATGGAGATTTATTTTTACTTTT
AGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAA
TTTTAAATATTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACGATGGATATTGAAAATATAGA
ATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTATCTAAAACAACTTCGTTAGATCCCGTAAAGCCAGTAGATG
TATTATTAACAGAAAATGATATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAAAAAAATCATCTAAAATTGAC
ACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAATGGACACTTAGAACCTGAGTTACAAGATGAACAGCCAATTA
ATATATTAGGATCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAAATAATTATTCAAGATATTGCGATGAAT
GAACAGCAAATATTAGATTATAGAAGTAAGAGAAAAAGTTTACCTTTTACAGAAAATGATGAAAATATTGTCGTGCTTAT
TCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTAT
TAAGAGATTTACCAATGTCAATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGTTATAAAGCATTAATAACTAA
ACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTGAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGT
TGAAAGTTTTCTTAGACTTAAAATTTGTAACACAAGAAGATAGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGA
AATAAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

DHHA1 domain (DHHA1)

(312-436)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(563-752)

RecJ OB domain (RecJ_OB)

(452-556)

DHH family (DHH)

(81-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

38.015

100

0.39
  recJ Streptococcus pneumoniae strain D39V

37.845

100

0.38