Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   SB45_RS08615 Genome accession   NZ_CP010556
Coordinates   1798654..1799088 (+) Length   144 a.a.
NCBI ID   WP_003154085.1    Uniprot ID   -
Organism   Bacillus velezensis strain L-H15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1793654..1804088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SB45_RS08610 (SB45_08610) - 1797154..1798365 (-) 1212 WP_003154086.1 cytochrome P450 -
  SB45_RS08615 (SB45_08615) nucA/comI 1798654..1799088 (+) 435 WP_003154085.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  SB45_RS08620 (SB45_08620) - 1799148..1799903 (+) 756 WP_003154084.1 YoaK family protein -
  SB45_RS08625 (SB45_08625) - 1799937..1800299 (-) 363 WP_003154082.1 hypothetical protein -
  SB45_RS08630 (SB45_08630) - 1800491..1801819 (-) 1329 WP_043867149.1 S8 family peptidase -
  SB45_RS08635 (SB45_08635) - 1801999..1802232 (+) 234 WP_024085328.1 hypothetical protein -
  SB45_RS08640 (SB45_08640) - 1802488..1803195 (+) 708 WP_043867150.1 poly-gamma-glutamate hydrolase family protein -
  SB45_RS08645 (SB45_08645) - 1803256..1803708 (+) 453 WP_043867151.1 OsmC family protein -
  SB45_RS08650 (SB45_08650) - 1803722..1804075 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15509.42 Da        Isoelectric Point: 6.8606

>NTDB_id=139257 SB45_RS08615 WP_003154085.1 1798654..1799088(+) (nucA/comI) [Bacillus velezensis strain L-H15]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=139257 SB45_RS08615 WP_003154085.1 1798654..1799088(+) (nucA/comI) [Bacillus velezensis strain L-H15]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTATGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment