Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   SB45_RS01680 Genome accession   NZ_CP010556
Coordinates   309263..309700 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain L-H15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 304263..314700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SB45_RS19935 - 304842..304967 (-) 126 Protein_287 hypothetical protein -
  SB45_RS01650 (SB45_01650) - 305120..306322 (+) 1203 WP_043866820.1 GTP-binding protein -
  SB45_RS01655 (SB45_01655) - 306387..307523 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  SB45_RS01660 (SB45_01660) - 307538..307972 (+) 435 WP_043866821.1 RDD family protein -
  SB45_RS01665 (SB45_01665) - 308044..308364 (+) 321 WP_014304315.1 YckD family protein -
  SB45_RS01670 (SB45_01670) - 308583..308802 (+) 220 Protein_292 family 1 glycosylhydrolase -
  SB45_RS01675 (SB45_01675) nin/comJ 308843..309241 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  SB45_RS01680 (SB45_01680) nucA/comI 309263..309700 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  SB45_RS01685 (SB45_01685) hxlB 310059..310616 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  SB45_RS01690 (SB45_01690) hxlA 310613..311248 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  SB45_RS01695 (SB45_01695) - 311480..311842 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=139229 SB45_RS01680 WP_003156588.1 309263..309700(-) (nucA/comI) [Bacillus velezensis strain L-H15]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=139229 SB45_RS01680 WP_003156588.1 309263..309700(-) (nucA/comI) [Bacillus velezensis strain L-H15]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment