Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RR42_RS02085 Genome accession   NZ_CP010536
Coordinates   463217..464692 (-) Length   491 a.a.
NCBI ID   WP_043343436.1    Uniprot ID   A0A643FNT8
Organism   Cupriavidus basilensis strain 4G11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458217..469692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR42_RS02055 (RR42_m0421) - 458674..459351 (-) 678 WP_043343427.1 LON peptidase substrate-binding domain-containing protein -
  RR42_RS02060 (RR42_m0422) rapZ 459418..460335 (-) 918 WP_043343429.1 RNase adapter RapZ -
  RR42_RS02065 (RR42_m0423) - 460455..460778 (-) 324 WP_043343431.1 PsiF family protein -
  RR42_RS02070 (RR42_m0424) hprK 460970..461941 (-) 972 WP_006159437.1 HPr(Ser) kinase/phosphatase -
  RR42_RS02075 (RR42_m0425) ptsN 461977..462432 (-) 456 WP_006159436.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RR42_RS02080 (RR42_m0427) hpf 462817..463167 (-) 351 WP_006159435.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RR42_RS02085 (RR42_m0428) rpoN/rpoN1 463217..464692 (-) 1476 WP_043343436.1 RNA polymerase factor sigma-54 Machinery gene
  RR42_RS02090 (RR42_m0429) lptB 464831..465616 (-) 786 WP_043343439.1 LPS export ABC transporter ATP-binding protein -
  RR42_RS02095 (RR42_m0430) lptA 465641..466285 (-) 645 WP_043343440.1 lipopolysaccharide transport periplasmic protein LptA -
  RR42_RS02100 (RR42_m0431) lptC 466340..466966 (-) 627 WP_043343442.1 LPS export ABC transporter periplasmic protein LptC -
  RR42_RS02105 (RR42_m0432) - 466982..467965 (-) 984 WP_043343445.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 54787.14 Da        Isoelectric Point: 4.9180

>NTDB_id=139141 RR42_RS02085 WP_043343436.1 463217..464692(-) (rpoN/rpoN1) [Cupriavidus basilensis strain 4G11]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWLDSPLRVSSDGSVNLQSAPAPAPADGQ
GTSENRPDGAPEADDSYSDSNEDYGSDWSLDDFARRPQGEDDEKTPMQLRDAEPTLREHLMEQLAPLKISLRDKGLAIFL
VESLDDDGYLSTTLEEICSELPEELEFEVDEIQAVLTLLQSFDPPGVGARNAAECLALQLRRLKHPQRELALSIVTNHLE
LLAVRDYTRLKKALQVDEPELKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKLSGGWIAHLNPDVMPRLRINDMYAQ
ILRGAKGDAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRVT
TNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDARNPLSDSRIAELLGEQGFVVARRTVAKYREAL
KIPAVNLRKTL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=139141 RR42_RS02085 WP_043343436.1 463217..464692(-) (rpoN/rpoN1) [Cupriavidus basilensis strain 4G11]
ATGAAACCGTCGCTACAGCTCCGCCTCTCGCAGCATCTCGCGCTTACGCCGCAACTCCAGCAGTCGATCCGGCTGCTGCA
ACTGTCCACGCTTGAACTGCAGCAGGAAGTGGAACAGGCGCTGACCGAGAACCCCTTGCTCGAGCGTGAGAACGACTGGC
TGGACAGCCCGCTGCGCGTGTCTTCGGACGGCTCGGTGAACCTGCAGAGCGCGCCCGCGCCGGCGCCAGCTGACGGCCAG
GGCACCAGCGAGAACCGCCCCGATGGCGCCCCCGAAGCCGACGACAGCTACTCTGACAGCAATGAGGATTACGGCAGCGA
CTGGAGCCTGGACGACTTCGCCCGCCGCCCGCAAGGCGAAGACGACGAAAAAACCCCGATGCAGCTGCGCGACGCCGAGC
CCACGCTGCGTGAACACCTCATGGAGCAGCTGGCACCGCTCAAGATTTCCCTGCGCGACAAAGGGCTGGCTATCTTCCTG
GTCGAATCCCTGGACGATGACGGCTACCTGAGCACCACGCTCGAGGAAATCTGCAGCGAGCTGCCGGAGGAGCTCGAGTT
CGAGGTCGACGAGATCCAGGCTGTGCTCACGCTGCTGCAGAGCTTCGATCCGCCGGGCGTCGGGGCGCGCAATGCCGCCG
AGTGCCTGGCGCTGCAATTGCGCAGGCTCAAGCATCCGCAGCGCGAACTGGCGCTGAGCATCGTCACCAACCACCTCGAG
CTGCTGGCGGTGCGGGATTACACGCGCCTGAAAAAAGCGCTGCAGGTGGACGAACCTGAACTCAAGGCGGCGCATGAATT
GATCCGCTCGCTCGCGCCCTATCCCGGCCATGCCTACAGCCGGCCAGAAGCGGACTTCGTGGTGCCCGATGTTTTTGTTC
GAAAGTTGAGCGGCGGATGGATCGCCCACCTCAACCCGGATGTGATGCCGCGGCTACGCATCAACGACATGTACGCGCAG
ATTTTGCGCGGTGCAAAAGGCGACGCTGGCGCGGCCGGACTGCAGCAAAAACTGCAAGAAGCACGCTGGTTGATCAAGAA
TATCCAGCAAAGGTTCGACACGATATTGCGCGTCGCACAAGCCATTGTCGAACGTCAAAAGAACTTCTTCACCCACGGTG
AAATTGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTTTACACGAGTCCACCATCTCTCGGGTGACT
ACCAATAAATACATGGCAACGCCGATGGGGACTTTCGAGTTGAAGTATTTTTTCGGCTCTCACGTATCCACAGAAACCGG
CGGCGCGGCCTCATCAACGGCAATCCGTGCGTTGATCAAGCAATTGATAGGAGCCGAAGACGCTAGGAACCCCTTGTCCG
ACAGCCGGATTGCCGAATTGCTCGGCGAGCAGGGCTTTGTGGTGGCACGGCGTACCGTTGCCAAATACCGCGAAGCGCTC
AAGATTCCAGCGGTAAATCTCCGTAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(334-489)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-318)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A643FNT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

78.758

100

0.8

Multiple sequence alignment