Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   SG18_RS20480 Genome accession   NZ_CP010518
Coordinates   4565525..4567012 (-) Length   495 a.a.
NCBI ID   WP_042114309.1    Uniprot ID   A0A0B5FF78
Organism   Pandoraea apista strain TF81F4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4560525..4572012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SG18_RS20450 (SG18_11595) mutY 4560580..4561752 (+) 1173 WP_042114301.1 A/G-specific adenine glycosylase -
  SG18_RS20455 (SG18_11600) rapZ 4561754..4562635 (-) 882 WP_042114302.1 RNase adapter RapZ -
  SG18_RS20460 (SG18_11605) - 4562808..4563134 (-) 327 WP_042114303.1 PsiF family protein -
  SG18_RS20465 (SG18_11610) hprK 4563294..4564259 (-) 966 WP_010804807.1 HPr(Ser) kinase/phosphatase -
  SG18_RS20470 (SG18_11615) ptsN 4564337..4564792 (-) 456 WP_042114306.1 PTS IIA-like nitrogen regulatory protein PtsN -
  SG18_RS20475 (SG18_11620) hpf 4565029..4565382 (-) 354 WP_039373742.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SG18_RS20480 (SG18_11625) rpoN/rpoN1 4565525..4567012 (-) 1488 WP_042114309.1 RNA polymerase factor sigma-54 Machinery gene
  SG18_RS20485 (SG18_11630) lptB 4567107..4567877 (-) 771 WP_042117810.1 LPS export ABC transporter ATP-binding protein -
  SG18_RS20490 (SG18_25870) lptA 4567903..4568604 (-) 702 WP_082117758.1 lipopolysaccharide transport periplasmic protein LptA -
  SG18_RS20495 (SG18_11640) lptC 4568601..4569260 (-) 660 WP_042114310.1 LPS export ABC transporter periplasmic protein LptC -
  SG18_RS20500 (SG18_11645) - 4569285..4569857 (-) 573 WP_042114311.1 KdsC family phosphatase -
  SG18_RS20505 (SG18_11650) - 4569869..4570849 (-) 981 WP_042114313.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 55108.95 Da        Isoelectric Point: 5.1872

>NTDB_id=138861 SG18_RS20480 WP_042114309.1 4565525..4567012(-) (rpoN/rpoN1) [Pandoraea apista strain TF81F4]
MKPTLQLRTSQHLTLTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEREDDWLAGSVRVGTDGSLRNERATSVSSTNDG
AESANGRDEARELDGETRFDDNSQDNGGDWSLNDFARSGSTSDDDDNARPQLHVDHPNLREHLLAQLRLTKAGPRDRALV
AFLIESLDEDGYLGTTLEEIEAEMPEELALETEEINAALALLQSFDPAGVGARSPAECLRLQLQRLPASSVRTLSLRIVS
DHLELLAARDYTRLRRVLGASEDGLRAAHQLIRSLDPFPGAAYGTPQADYVVPDVLVRRQGSGWTAELNPDVMPRLRINE
MYARILRNNRNDPGTGNLQQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHESTI
SRVTTSKYMLTPFGTFELKYFFGSHVATEAGGAASSTAIRALIKQLIGAEDPQSPLSDSRIAELLAEQGFVVARRTVAKY
REAMRIPAVNLRKSL

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=138861 SG18_RS20480 WP_042114309.1 4565525..4567012(-) (rpoN/rpoN1) [Pandoraea apista strain TF81F4]
ATGAAACCGACTCTTCAACTCCGCACCTCGCAGCACCTGACCCTCACGCCGCAGTTGCAGCAATCGATCCGGTTGTTGCA
ACTGTCTACGCTCGAACTTCAGCAGGAAGTCGAGCACGCCCTCACCCAGAACCCGATGCTCGAACGCGAGGACGATTGGC
TCGCGGGCTCGGTACGTGTGGGCACGGACGGGTCGCTGCGCAACGAGCGGGCAACGTCGGTCAGCAGCACGAACGACGGC
GCCGAGTCCGCCAACGGCCGTGACGAAGCCCGCGAACTCGACGGCGAGACCCGGTTCGACGACAACTCGCAAGACAATGG
CGGCGACTGGAGCCTGAACGACTTCGCCCGCTCCGGCAGCACCTCGGACGACGACGACAACGCGCGTCCGCAGCTGCACG
TCGACCATCCCAACCTTCGCGAACACCTGCTGGCGCAGTTGCGCCTGACCAAGGCCGGTCCGCGCGACCGTGCGCTGGTG
GCGTTCCTGATCGAATCGCTCGACGAAGACGGCTATCTCGGCACCACGCTCGAAGAAATCGAGGCAGAAATGCCCGAAGA
ACTGGCGCTGGAGACCGAGGAAATCAACGCCGCGCTCGCGCTGCTGCAAAGCTTCGACCCGGCGGGTGTGGGCGCCCGCA
GCCCGGCCGAATGCCTTCGCCTGCAACTCCAGCGCCTGCCTGCGAGCAGCGTTCGCACGCTCTCGCTGCGCATCGTGAGC
GATCACCTGGAACTGCTCGCCGCACGCGATTACACCCGTCTGCGCCGCGTGCTGGGGGCGAGCGAAGACGGCTTGCGTGC
CGCCCATCAACTGATTCGTTCGCTCGATCCGTTCCCCGGTGCCGCGTATGGCACGCCGCAGGCCGATTACGTCGTGCCCG
ATGTGCTCGTGCGCAGACAGGGCAGCGGCTGGACCGCCGAGCTCAATCCCGACGTCATGCCGCGCCTGCGCATCAACGAG
ATGTACGCGCGCATTCTGCGCAATAACCGCAACGACCCGGGTACCGGCAACTTGCAACAACAACTTCAGGAAGCCCGCTG
GCTGATCAAAAACATCCAGCAGCGCTTCGATACGATCCTGCGCGTGGCGCAAGCGATCGTCGAGCGTCAAAAGAACTTCT
TCACGCATGGCGAAATCGGCATGCGGCCCTTGGTTTTACGCGAGATTGCTGATACGCTGGGTTTACACGAATCCACGATT
TCACGCGTGACCACCAGTAAGTACATGCTCACTCCATTTGGAACGTTTGAGCTGAAGTACTTCTTTGGTTCACATGTGGC
AACGGAAGCCGGGGGCGCAGCGTCATCGACCGCCATCCGCGCACTCATCAAGCAACTCATAGGAGCAGAAGACCCGCAGA
GCCCCCTTTCCGACAGCCGTATCGCGGAACTGCTGGCAGAGCAAGGGTTCGTGGTGGCGCGGCGCACCGTCGCGAAATAC
CGCGAGGCCATGCGAATTCCGGCAGTGAACTTGCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-322)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B5FF78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.864

100

0.687

Multiple sequence alignment