Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   DW68_RS01685 Genome accession   NZ_CP013124
Coordinates   364635..366419 (-) Length   594 a.a.
NCBI ID   WP_013717654.1    Uniprot ID   A0ABM5VRL1
Organism   Ectopseudomonas mendocina S5.2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359635..371419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DW68_RS01660 (DW68_001660) - 360348..361319 (+) 972 WP_013717659.1 sensor domain-containing diguanylate cyclase -
  DW68_RS01665 (DW68_001665) - 361745..363070 (+) 1326 WP_013717658.1 OprD family porin -
  DW68_RS01670 (DW68_001670) - 363146..363484 (-) 339 WP_017363892.1 DUF2388 domain-containing protein -
  DW68_RS01675 (DW68_001675) - 363572..363826 (-) 255 WP_017363891.1 NfeD family protein -
  DW68_RS01680 (DW68_001680) - 363974..364660 (+) 687 WP_394327096.1 murein L,D-transpeptidase catalytic domain family protein -
  DW68_RS01685 (DW68_001685) pilB/pilB1 364635..366419 (-) 1785 WP_013717654.1 GspE/PulE family protein Machinery gene
  DW68_RS01690 (DW68_001690) - 366542..366868 (-) 327 WP_013717653.1 hypothetical protein -
  DW68_RS01695 (DW68_001695) - 366996..367463 (+) 468 WP_017363889.1 Lrp/AsnC family transcriptional regulator -
  DW68_RS01700 (DW68_001700) - 367609..368280 (-) 672 WP_017363888.1 DUF502 domain-containing protein -
  DW68_RS01705 (DW68_001705) dctM 368486..369766 (-) 1281 WP_036994768.1 C4-dicarboxylate TRAP transporter large permease protein DctM -
  DW68_RS01710 (DW68_001710) - 369766..370392 (-) 627 WP_017364065.1 TRAP transporter small permease -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 65768.47 Da        Isoelectric Point: 6.7381

>NTDB_id=138809 DW68_RS01685 WP_013717654.1 364635..366419(-) (pilB/pilB1) [Ectopseudomonas mendocina S5.2]
MSAFTATAPDRVLDLGDLLRELVAQGRVEQEVAEQCLAIRRSSLNNAQHPLEFLAAQQVDDLSRPGKKLDLESLTVWLAN
FAGQPYLRIDPLKIDVPAITPLMSYAFAQRHKILAVAVDSESVTIASSQPLVHSWEANLVHVLKRPIKRVVANPSDIQRF
TTEFYRLARSVSGANATDQKVSGVGNFEQLLSLGAQDQEPDANDAHIVNIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHTVYQFPAQVTMAVVSRLKNLGRMNIAEKRKPQDGRVKTKTPDGGEVELRLSTLPTAFGEKMVMRIFDPEVLLKS
PDQLGFSPEDLRRWASMTSQPNGIILVTGPTGSGKTTTLYTTLKQLATPEVNVCTIEDPIEMIEGAFNQMQVQHNIDLNF
ASGVRALMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDAPSAITRLLELGVPHYLLKATILGVMAQRLVRT
LCPHCKAPVQLDEDTWNGLTRPWSSPLPTQAHHAVGCLECRETGYRGRAGVYEIMLINDAIKPLISADTDLTLLRRAAFK
DGMRSLRLSGAQKVAAGLTTIEEVLRVTPQSEQR

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=138809 DW68_RS01685 WP_013717654.1 364635..366419(-) (pilB/pilB1) [Ectopseudomonas mendocina S5.2]
ATGTCCGCCTTCACCGCTACCGCCCCTGACCGTGTACTGGATCTTGGCGACCTGCTGCGCGAGCTGGTGGCCCAAGGCCG
CGTCGAGCAGGAGGTGGCCGAGCAGTGCCTGGCGATTCGTCGCAGCTCGCTGAACAACGCCCAGCACCCTCTTGAGTTTC
TCGCCGCGCAACAGGTCGACGACCTGTCTCGGCCGGGCAAGAAGCTCGATCTGGAAAGCCTCACGGTCTGGCTGGCCAAC
TTCGCCGGCCAACCCTACCTGCGCATCGACCCGCTGAAAATCGACGTACCGGCCATCACCCCGCTGATGTCCTACGCCTT
CGCCCAGCGCCACAAGATTCTCGCCGTGGCAGTGGACAGCGAGAGCGTGACCATCGCCAGCAGCCAGCCGCTTGTGCACA
GCTGGGAAGCCAACCTGGTCCACGTACTCAAGCGCCCGATCAAGCGCGTGGTGGCCAACCCCAGCGATATCCAGCGCTTC
ACCACCGAGTTCTATCGTCTGGCGCGTTCGGTCAGCGGCGCCAATGCCACCGATCAAAAGGTCAGCGGTGTCGGCAACTT
CGAACAATTGCTCAGCCTCGGCGCCCAGGACCAAGAGCCGGATGCCAACGACGCGCACATCGTCAACATCGTCGACTGGC
TGTTCCAGTACGCCTTCCAGCAGCGCGCCAGCGATATCCACATCGAACCGCGCAGGGAACAGGGCACGGTGCGCTTTCGC
ATCGACGGCGTATTGCACACCGTCTACCAGTTCCCCGCGCAGGTCACCATGGCGGTAGTCAGCCGCCTGAAGAACCTCGG
CCGCATGAACATCGCCGAGAAGCGCAAACCGCAGGATGGCCGGGTCAAGACCAAGACCCCGGACGGCGGCGAGGTAGAGC
TGCGTCTTTCCACGCTGCCGACAGCCTTCGGCGAGAAGATGGTGATGCGTATCTTCGACCCGGAAGTGCTGCTGAAGAGC
CCGGATCAGCTTGGTTTCTCCCCCGAAGACCTGCGCCGCTGGGCGAGCATGACCAGTCAGCCCAATGGCATCATCCTGGT
CACCGGCCCCACCGGCTCAGGCAAGACCACCACGCTCTACACCACACTCAAGCAACTGGCGACGCCGGAAGTGAACGTGT
GCACCATCGAAGACCCCATCGAGATGATCGAAGGCGCCTTCAACCAGATGCAGGTGCAGCACAATATCGACCTGAATTTC
GCCAGCGGCGTGCGTGCACTGATGCGTCAGGACCCGGATATCATCATGGTCGGCGAGATACGTGATCTGGAAACAGCGGA
AATGGCCATCCAGGCTGCGCTGACTGGCCACCTGGTGCTCTCCACCCTGCACACCAACGACGCCCCCAGCGCCATCACCC
GCCTGCTCGAACTGGGCGTGCCGCATTACCTGCTCAAGGCCACCATTCTTGGCGTCATGGCCCAGCGCCTGGTGCGCACC
CTGTGCCCGCACTGCAAGGCGCCGGTACAACTGGACGAAGATACCTGGAACGGCCTGACGCGCCCCTGGAGCTCACCGCT
GCCGACTCAGGCGCACCACGCAGTCGGTTGCCTGGAATGCCGCGAAACCGGCTATCGTGGCCGGGCCGGCGTCTACGAGA
TCATGCTCATCAACGACGCGATCAAGCCACTGATCAGCGCCGACACAGACCTTACACTGCTACGCCGGGCCGCATTCAAG
GACGGTATGCGCAGCCTGCGCCTGTCCGGTGCGCAGAAGGTTGCCGCGGGGCTGACCACTATCGAAGAAGTGCTGCGCGT
CACTCCACAGAGCGAACAGCGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(216-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.304

100

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.815

90.909

0.362