Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   RB99_RS05840 Genome accession   NZ_CP010502
Coordinates   1089971..1090921 (+) Length   316 a.a.
NCBI ID   WP_002933188.1    Uniprot ID   A0A5T1IXP3
Organism   Campylobacter jejuni strain CJ677CC014     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1069646..1089912 1089971..1090921 flank 59


Gene organization within MGE regions


Location: 1069646..1090921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB99_RS05730 (RB99_05735) pglI 1069646..1070575 (-) 930 WP_002932672.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  RB99_RS05735 (RB99_05740) pglH 1070568..1071647 (-) 1080 WP_072225867.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  RB99_RS05740 (RB99_05745) pglK 1071644..1073338 (-) 1695 WP_002932660.1 ABC-type lipopolysaccharide transporter PglK -
  RB99_RS05745 (RB99_05750) galE 1073332..1074318 (-) 987 WP_002891881.1 UDP-glucose 4-epimerase GalE -
  RB99_RS05750 (RB99_05755) - 1074377..1075171 (-) 795 WP_002932659.1 3'-5' exonuclease -
  RB99_RS05755 (RB99_05760) waaC 1075238..1076266 (+) 1029 WP_002932658.1 lipopolysaccharide heptosyltransferase I -
  RB99_RS05760 (RB99_05765) htrB 1076256..1077134 (+) 879 WP_002932656.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  RB99_RS05765 (RB99_05770) - 1077131..1077832 (+) 702 WP_002932655.1 glycosyltransferase family 2 protein -
  RB99_RS05770 (RB99_05775) rfbA 1077843..1078721 (+) 879 WP_002932653.1 glucose-1-phosphate thymidylyltransferase RfbA -
  RB99_RS05775 (RB99_05780) rfbB 1078721..1079752 (+) 1032 WP_002932647.1 dTDP-glucose 4,6-dehydratase -
  RB99_RS05780 (RB99_05785) wlaRA 1079755..1080171 (+) 417 WP_002932645.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRA -
  RB99_RS05785 (RB99_05790) wlaRB 1080193..1080597 (+) 405 WP_002932644.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRB -
  RB99_RS05790 (RB99_05795) - 1080587..1081057 (+) 471 WP_002932643.1 acyltransferase -
  RB99_RS05795 (RB99_05800) wlaRD 1081050..1081868 (+) 819 WP_002866154.1 class E lipooligosaccharide biosynthesis sugar-formyltransferase WlaRD -
  RB99_RS05800 (RB99_05805) - 1081887..1082963 (+) 1077 WP_002932642.1 glycosyltransferase family A protein -
  RB99_RS05805 (RB99_05810) wlaRG 1082960..1084045 (+) 1086 WP_002932641.1 class E lipooligosaccharide biosynthesis aminotransferase WlaRG -
  RB99_RS05810 (RB99_05815) - 1084079..1085332 (-) 1254 WP_002932640.1 DUF2972 domain-containing protein -
  RB99_RS05815 (RB99_05820) - 1085329..1086342 (-) 1014 WP_002932637.1 hypothetical protein -
  RB99_RS05820 (RB99_05825) - 1086413..1087186 (+) 774 WP_002866160.1 glycosyltransferase family 25 protein -
  RB99_RS05825 (RB99_05830) wlaTB 1087179..1088135 (+) 957 WP_002869613.1 class E lipooligosaccharide biosynthesis protein WlaTB -
  RB99_RS05830 (RB99_05835) wlaTC 1088132..1089091 (+) 960 WP_048818625.1 class E lipooligosaccharide biosynthesis protein WlaTC -
  RB99_RS05835 (RB99_05840) - 1089094..1089912 (-) 819 WP_002933186.1 glycosyltransferase family 2 protein -
  RB99_RS05840 (RB99_05845) waaF 1089971..1090921 (+) 951 WP_002933188.1 lipopolysaccharide heptosyltransferase II Regulator

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36201.62 Da        Isoelectric Point: 10.1851

>NTDB_id=138601 RB99_RS05840 WP_002933188.1 1089971..1090921(+) (waaF) [Campylobacter jejuni strain CJ677CC014]
MKIFIHLPTWLGDAVMASPALYTIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLHKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHSKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENATLVHLDLACMPCMQKTCPLKHHKCMKDLKPERVIEEIKKLSTP

Nucleotide


Download         Length: 951 bp        

>NTDB_id=138601 RB99_RS05840 WP_002933188.1 1089971..1090921(+) (waaF) [Campylobacter jejuni strain CJ677CC014]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTACAAACAAGCCCTATCTTTACACAAAGAACTTGGTAAAATTGATCTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAATAAGCA
CAAAGAAGAACATCAAGTTTTAAAATATCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTCTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGTTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGTCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTTATCACAAATGACAGT
GGTCCTATGCACCTAAGTGCAGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCCACAAAATTTACCCAAACTTCACC
TTGGCAAAACGAAAACGCAACATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGTCCTTTAAAAC
ACCATAAATGTATGAAAGATTTAAAGCCTGAAAGAGTGATAGAAGAAATTAAAAAATTAAGCACCCCATAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5T1IXP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

95.208

99.051

0.943

Multiple sequence alignment