Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   AKK22_RS26350 Genome accession   NZ_CP013029
Coordinates   4970078..4970746 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 2012C-4227     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4965078..4975746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKK22_RS26320 (AKK22_25815) yicC 4965097..4965960 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  AKK22_RS26325 (AKK22_25820) rph 4966087..4966803 (+) 717 WP_001247089.1 ribonuclease PH -
  AKK22_RS26330 (AKK22_25825) pyrE 4966869..4967510 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  AKK22_RS26335 (AKK22_25830) slmA 4967547..4968143 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  AKK22_RS26340 (AKK22_25835) dut 4968250..4968708 (-) 459 WP_000976070.1 dUTP diphosphatase -
  AKK22_RS26345 (AKK22_25840) coaBC 4968686..4969906 (-) 1221 WP_032207408.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  AKK22_RS26350 (AKK22_25845) radC 4970078..4970746 (+) 669 WP_001297375.1 RadC family protein Machinery gene
  AKK22_RS26355 (AKK22_25850) rpmB 4970963..4971199 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  AKK22_RS26360 (AKK22_25855) rpmG 4971220..4971387 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  AKK22_RS26365 (AKK22_25860) mutM 4971485..4972294 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  AKK22_RS26370 (AKK22_25865) coaD 4972333..4972812 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  AKK22_RS26375 (AKK22_25870) waaA 4972820..4974097 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  AKK22_RS26380 (AKK22_25875) rfaQ 4974510..4975568 (+) 1059 WP_071887534.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=138486 AKK22_RS26350 WP_001297375.1 4970078..4970746(+) (radC) [Escherichia coli strain 2012C-4227]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=138486 AKK22_RS26350 WP_001297375.1 4970078..4970746(+) (radC) [Escherichia coli strain 2012C-4227]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment