Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   MJ49_RS12715 Genome accession   NZ_CP013025
Coordinates   2378960..2379628 (+) Length   222 a.a.
NCBI ID   WP_001318112.1    Uniprot ID   -
Organism   Escherichia coli strain 2009C-3133     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2373960..2384628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ49_RS12685 (MJ49_11960) yicC 2373979..2374842 (-) 864 WP_000621330.1 YicC/YloC family endoribonuclease -
  MJ49_RS12690 (MJ49_11965) rph 2374969..2375685 (+) 717 WP_001247093.1 ribonuclease PH -
  MJ49_RS12695 (MJ49_11970) pyrE 2375751..2376392 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  MJ49_RS12700 (MJ49_11975) slmA 2376429..2377025 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  MJ49_RS12705 (MJ49_11980) dut 2377132..2377590 (-) 459 WP_000976070.1 dUTP diphosphatase -
  MJ49_RS12710 (MJ49_11985) coaBC 2377568..2378788 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  MJ49_RS12715 (MJ49_11990) radC 2378960..2379628 (+) 669 WP_001318112.1 DNA repair protein RadC Machinery gene
  MJ49_RS12720 (MJ49_11995) rpmB 2379845..2380081 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  MJ49_RS12725 (MJ49_12000) rpmG 2380102..2380269 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  MJ49_RS12730 (MJ49_12005) mutM 2380367..2381176 (+) 810 WP_001114537.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  MJ49_RS12735 (MJ49_12010) coaD 2381215..2381694 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  MJ49_RS12740 (MJ49_12015) waaA 2381702..2382979 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  MJ49_RS12745 (MJ49_12020) rfaQ 2383392..2384450 (+) 1059 WP_000360409.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25286.36 Da        Isoelectric Point: 7.9755

>NTDB_id=138368 MJ49_RS12715 WP_001318112.1 2378960..2379628(+) (radC) [Escherichia coli strain 2009C-3133]
MKNNAQLLMPREKMLRFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=138368 MJ49_RS12715 WP_001318112.1 2378960..2379628(+) (radC) [Escherichia coli strain 2009C-3133]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAGGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAGTTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAATGCTTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment