Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACT75_RS05360 Genome accession   NZ_CP012959
Coordinates   1117160..1119358 (-) Length   732 a.a.
NCBI ID   WP_061887487.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 624     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1112160..1124358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT75_RS05345 (ACT75_05340) - 1114228..1115424 (-) 1197 WP_005542862.1 efflux RND transporter periplasmic adaptor subunit -
  ACT75_RS05350 (ACT75_05345) - 1115450..1116016 (-) 567 WP_005542860.1 TetR/AcrR family transcriptional regulator -
  ACT75_RS05355 (ACT75_05350) ftsN 1116217..1117050 (-) 834 WP_005542858.1 cell division protein FtsN -
  ACT75_RS05360 (ACT75_05355) priA 1117160..1119358 (-) 2199 WP_061887487.1 primosomal protein N' Machinery gene
  ACT75_RS05365 (ACT75_05360) yibB 1119585..1120448 (+) 864 WP_005542853.1 protein YibB -
  ACT75_RS05370 (ACT75_05365) - 1120455..1121459 (-) 1005 WP_005542849.1 capsular polysaccharide synthesis protein -
  ACT75_RS05375 (ACT75_05370) - 1121468..1122316 (-) 849 WP_005542847.1 glycosyltransferase family 25 protein -
  ACT75_RS05380 (ACT75_05375) rpmE 1122574..1122786 (+) 213 WP_005542845.1 50S ribosomal protein L31 -
  ACT75_RS05385 (ACT75_05380) - 1122849..1123541 (+) 693 WP_005542843.1 glycosyltransferase family 25 protein -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 82508.72 Da        Isoelectric Point: 9.2068

>NTDB_id=137728 ACT75_RS05360 WP_061887487.1 1117160..1119358(-) (priA) [Aggregatibacter actinomycetemcomitans strain 624]
MSFARIALPVPLHRYFDYILPTSLNVVVGARVWVPFGTQKRVGVVVEVAEQSDVPVEQLKPVQAYLDEVSLFSPQSWQFL
QWAAGYYHAPLGEVLSQALPVKLRQGESAVEKPSVFWKPTALGEQALTQGLLKRAKKQLEALQAANDLPEKGNNPFSAAI
WSALKAKDYVTEVILETEIQTWQQQLGDKPLVNKADRLTLNKPQALVFSQLKFTQGFVPWLLDGVTGSGKTEIYLQFIED
ILQQGKQVLVLVPEIGLTPQTVRRFQARFNVVIDVLHSNLNDTQRLQVWQRARNGQSAVVIGTRSALFTQFAELGAIIID
EEHDASFKQQDGWRYHARDLAIVYAKQLNIPILMGSATPSLESLNNVQSGKYRHLSLSEKANNSTALKQQIIDLKRQPIH
NGLSDVLLKRMQAHLEKGNQVLLFLNRRGFAPVLLCHECGWMAECRHCDKPYTYHQQHHVLRCHHCASQKPIPRQCGNCG
STQLITTGLGTEQLEATLKEAFPNYGIARIDRDTTARKGKLEDYLEDINQGKSQILIGTQMLAKGHHFPNVTLVALVNVD
SALFSLDFRAEERLAQLYMQVAGRAGRGSQQGEVLLQTHYPEHPLLQSLLQKGYSQFAQNALALRKSIGLPPFGSQALFK
AQSRYREEAEQLLAQFVEYLQQIKRNTAPELQILGPMPAPFSKKAGQYRWQLLLQHPSRAALQQVLTEFWRYQGDQPTKV
RWILDVDPLDLS

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=137728 ACT75_RS05360 WP_061887487.1 1117160..1119358(-) (priA) [Aggregatibacter actinomycetemcomitans strain 624]
ATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCCTGCCGACGTCGCTGAATGTTGT
CGTGGGGGCGCGGGTGTGGGTGCCGTTTGGGACGCAGAAACGTGTTGGCGTGGTGGTTGAGGTGGCGGAGCAGTCGGATG
TGCCTGTGGAGCAGTTGAAACCGGTTCAGGCTTATCTTGATGAGGTTTCCTTATTTTCCCCGCAAAGTTGGCAGTTTTTG
CAGTGGGCAGCGGGGTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGA
AAGTGCGGTGGAAAAACCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGACGCAGGGTTTATTGAAAC
GCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAAAAAGGCAATAACCCTTTTAGTGCGGCGATT
TGGTCGGCGCTGAAAGCCAAAGATTACGTCACGGAAGTTATTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGG
CGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACGTTAAACAAGCCGCAAGCGTTAGTGTTCAGTCAGTTGAAATTTA
CTCAGGGTTTTGTCCCGTGGTTGTTGGACGGCGTGACGGGTTCCGGCAAAACAGAAATCTATTTGCAATTTATCGAAGAT
ATTTTGCAGCAGGGCAAGCAGGTGCTGGTGTTGGTGCCGGAAATCGGTTTAACGCCACAAACGGTACGGCGTTTTCAGGC
GCGTTTTAATGTGGTCATTGACGTGCTGCATTCCAATTTGAACGATACTCAGCGTTTGCAGGTGTGGCAGCGGGCGCGCA
ATGGGCAAAGTGCGGTCGTTATCGGCACCCGATCTGCGTTGTTCACGCAATTCGCCGAGTTGGGCGCGATTATTATTGAT
GAAGAACATGATGCCTCTTTCAAACAGCAGGACGGCTGGCGTTATCACGCGCGCGATTTGGCGATTGTGTATGCGAAACA
GCTGAATATCCCGATTTTAATGGGATCGGCAACGCCAAGTTTGGAAAGTCTGAATAACGTGCAAAGCGGTAAATATCGCC
ATTTGTCGTTGTCGGAAAAAGCCAATAATTCCACCGCACTTAAACAGCAGATTATCGATTTAAAACGTCAACCTATTCAC
AATGGGCTGTCCGATGTGTTGTTAAAACGTATGCAGGCGCATTTGGAAAAAGGCAATCAGGTGTTGCTATTTCTGAATCG
GCGCGGTTTTGCGCCGGTACTGTTGTGCCACGAATGCGGCTGGATGGCGGAATGTCGCCACTGCGATAAACCTTACACTT
ATCATCAGCAACATCATGTGTTGCGCTGCCATCATTGTGCTTCACAAAAGCCGATTCCACGCCAATGCGGCAATTGCGGT
TCCACCCAATTAATCACCACGGGCTTAGGCACGGAACAACTGGAAGCGACGTTAAAAGAGGCGTTCCCTAACTACGGCAT
TGCCCGTATCGACCGCGACACAACGGCACGTAAAGGCAAGCTGGAAGACTATTTGGAAGATATTAACCAAGGCAAAAGCC
AAATTCTTATCGGCACCCAAATGCTGGCGAAAGGGCATCATTTCCCGAATGTCACCTTAGTGGCATTGGTGAATGTGGAT
AGTGCTTTGTTTTCATTGGATTTTCGTGCGGAAGAACGATTGGCACAGCTTTATATGCAGGTGGCGGGACGTGCAGGGCG
CGGTTCGCAACAAGGGGAAGTGTTATTACAAACCCATTACCCCGAACACCCGTTACTGCAAAGTCTGTTACAAAAAGGCT
ATAGCCAATTTGCGCAAAATGCCTTGGCGTTGCGTAAAAGTATAGGCTTGCCGCCTTTCGGTTCGCAGGCATTATTCAAA
GCGCAAAGCCGTTATAGAGAAGAAGCTGAACAATTGCTGGCGCAGTTTGTCGAATATTTACAACAGATTAAGCGAAACAC
GGCGCCCGAGTTGCAAATTCTCGGCCCGATGCCGGCGCCGTTCAGTAAAAAAGCGGGGCAATATCGTTGGCAGCTATTGT
TGCAGCATCCTTCCAGAGCGGCGTTGCAGCAGGTGTTGACGGAATTTTGGCGTTATCAGGGCGATCAACCCACAAAAGTG
CGGTGGATTTTAGACGTGGATCCGTTGGATCTGAGTTAG

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(5-100)

Primosomal protein N C-terminal domain (PriA_C)

(632-728)

DEAD/DEAH box helicase (DEAD)

(221-365)

Helicase conserved C-terminal domain (Helicase_C)

(496-588)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

42.645

100

0.432