Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACT74_RS08740 Genome accession   NZ_CP012958
Coordinates   1815013..1817211 (+) Length   732 a.a.
NCBI ID   WP_005572398.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain VT1169     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1810013..1822211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT74_RS08715 (ACT74_08715) - 1810829..1811521 (-) 693 WP_005542843.1 glycosyltransferase family 25 protein -
  ACT74_RS08720 (ACT74_08720) rpmE 1811584..1811796 (-) 213 WP_005542845.1 50S ribosomal protein L31 -
  ACT74_RS08725 (ACT74_08725) - 1812054..1812902 (+) 849 WP_005566799.1 glycosyltransferase family 25 protein -
  ACT74_RS08730 (ACT74_08730) - 1812911..1813915 (+) 1005 WP_025298246.1 capsular polysaccharide synthesis protein -
  ACT74_RS08735 (ACT74_08735) yibB 1813922..1814785 (-) 864 WP_025298247.1 protein YibB -
  ACT74_RS08740 (ACT74_08740) priA 1815013..1817211 (+) 2199 WP_005572398.1 primosomal protein N' Machinery gene
  ACT74_RS08745 (ACT74_08745) ftsN 1817321..1818133 (+) 813 WP_005544930.1 cell division protein FtsN -
  ACT74_RS08750 (ACT74_08750) - 1818333..1818899 (+) 567 WP_005544928.1 TetR/AcrR family transcriptional regulator -
  ACT74_RS08755 (ACT74_08755) - 1818925..1820121 (+) 1197 WP_025298248.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 82334.66 Da        Isoelectric Point: 8.9347

>NTDB_id=137704 ACT74_RS08740 WP_005572398.1 1815013..1817211(+) (priA) [Aggregatibacter actinomycetemcomitans strain VT1169]
MSFARIALPVPLHRYFDYILPASLNAVVGARVWVPFGAQKRVGMVVEVAEQSDVPAEQLKTVLACIDEVSLFSPQSWQFL
QWAAGYYHAPLGEVLSQALPVKLRQGESAVENPSVFWKPTALGEQALAQGLLKRAKKQLEALQAANDLPEKGNNPFSAAI
WSVLKAKDYVTEVALETEIQTWQQQLGDKPLVNKADRLTLNKSQALVFSQLKFTQGFVPWLLNGVTGSGKTEIYLQFIEE
ILQQGKQVLVLVPEISLTPQTVRRFQARFNVVIDVLHSNLNDTQRLQVWQRARNGQSAVVIGTRSALFTQFSELGVIIID
EEHDASFKQQDGWRYHARDLAIVYAKQLNIPILMGSATPSLESLNNVQSGKYRHLSLSEKANNSTALKQQIIDLKRQPMH
NGLSDVLLKRMQAHLEKGNQVLLFLNRRGFAPVLLCHECGWMAECRHCDKPYTYHQQHHVLRCHHCASQKPIPRQCGNCG
STQLITTGLGTEQLEATLKDVFPHYGIARIDRDTTARKGKLEGYLEDINQGKSQILIGTQMLAKGHHFPNVTLVALVNVD
SALFSLDFRAEERLAQLYMQVAGRAGRGVQQGEVLLQTHYPEHPLLQSLLQKGYNQFAQNALALRKSMGLPPFGSQALFK
AQSRYSEEAEQLLAQFVEYLQQIKRNTAPELQILGPMPAPFSKKAGQYRWQLLLQHPSRAALQQVLMEFWCYQGDQPTKV
RWILDVDPLDLS

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=137704 ACT74_RS08740 WP_005572398.1 1815013..1817211(+) (priA) [Aggregatibacter actinomycetemcomitans strain VT1169]
ATGTCATTTGCCCGAATTGCGCTTCCTGTGCCGTTACATCGCTATTTTGATTATATCTTGCCGGCGTCGCTGAATGCTGT
CGTGGGGGCGAGGGTGTGGGTGCCGTTTGGGGCGCAGAAGCGTGTTGGCATGGTGGTTGAGGTGGCGGAGCAGTCGGATG
TGCCTGCGGAGCAGTTGAAGACAGTGCTGGCTTGCATTGATGAGGTTTCCTTATTTTCTCCGCAAAGCTGGCAGTTTTTG
CAGTGGGCGGCGGGGTATTATCACGCGCCCTTGGGGGAAGTGTTGTCGCAGGCGTTGCCGGTGAAATTGCGGCAGGGCGA
AAGTGCGGTGGAAAACCCAAGTGTTTTTTGGAAACCGACCGCACTTGGTGAGCAGGCATTGGCGCAGGGCTTATTGAAAC
GCGCGAAGAAGCAGCTTGAAGCGTTGCAGGCGGCGAATGATTTGCCGGAGAAAGGCAACAACCCTTTTAGTGCGGCGATT
TGGTCGGTGCTGAAAGCCAAAGATTACGTCACGGAAGTTGCTCTGGAAACGGAAATCCAAACCTGGCAGCAACAATTAGG
CGATAAGCCGCTTGTGAATAAGGCGGATCGTTTAACCTTAAACAAGTCGCAGGCGTTAGTGTTCAGTCAGTTGAAATTTA
CTCAGGGTTTTGTCCCGTGGTTGTTGAACGGCGTGACGGGTTCCGGCAAAACGGAAATCTATTTGCAATTTATCGAAGAG
ATTTTGCAGCAAGGCAAGCAAGTGCTGGTGTTGGTGCCGGAAATCAGTTTAACGCCACAAACGGTGCGGCGTTTTCAGGC
GCGTTTTAATGTGGTCATTGACGTGCTGCATTCCAATCTGAACGACACGCAGCGTTTGCAGGTGTGGCAGCGGGCGCGCA
ACGGGCAAAGTGCGGTCGTTATCGGCACCCGATCTGCGTTGTTCACGCAATTTTCCGAGTTGGGCGTGATTATTATTGAT
GAAGAACATGATGCCTCTTTCAAACAGCAGGACGGCTGGCGTTATCACGCGCGTGATTTGGCGATTGTGTATGCGAAACA
GCTGAATATCCCGATTTTAATGGGGTCGGCAACGCCAAGTTTGGAAAGCCTGAATAATGTGCAAAGCGGTAAATATCGCC
ATTTGTCGTTGTCGGAAAAAGCCAATAATTCCACCGCACTTAAACAGCAGATTATCGATTTAAAACGTCAACCTATGCAC
AACGGGCTGTCTGATGTGTTGTTAAAACGTATGCAGGCGCATTTGGAAAAAGGCAATCAGGTGTTGTTGTTTTTGAATCG
GCGCGGTTTTGCGCCGGTGCTGTTGTGCCACGAATGCGGCTGGATGGCGGAATGTCGCCATTGCGATAAGCCTTACACCT
ATCATCAGCAACATCATGTGTTGCGCTGCCATCATTGCGCTTCGCAGAAGCCGATTCCACGCCAATGCGGCAATTGCGGT
TCCACACAATTAATCACCACGGGCTTGGGTACGGAACAGCTGGAAGCCACCTTAAAAGACGTTTTTCCTCACTACGGCAT
TGCCCGCATCGACCGCGACACAACGGCACGTAAAGGCAAGCTGGAAGGCTATTTGGAAGATATTAACCAAGGCAAAAGTC
AAATTCTTATCGGCACTCAAATGCTGGCGAAAGGGCATCATTTCCCGAATGTCACGCTGGTGGCATTGGTGAATGTGGAT
AGCGCCTTATTTTCATTGGATTTTCGTGCGGAAGAACGATTGGCGCAGCTTTATATGCAGGTGGCGGGGCGTGCAGGGCG
TGGTGTACAGCAAGGCGAAGTGTTACTGCAAACCCATTACCCCGAGCACCCGTTACTGCAAAGTCTGTTACAAAAAGGTT
ATAACCAATTTGCGCAAAATGCCTTGGCGTTGCGTAAAAGTATGGGTTTGCCGCCTTTCGGTTCGCAGGCATTATTCAAG
GCGCAAAGCCGTTATAGCGAAGAAGCTGAACAATTGCTGGCGCAGTTTGTCGAATATTTACAACAGATTAAGCGAAACAC
GGCGCCCGAGTTGCAAATTCTCGGTCCGATGCCGGCGCCGTTCAGTAAAAAAGCGGGGCAATATCGTTGGCAGCTATTAC
TGCAGCATCCTTCCAGAGCGGCGTTGCAGCAGGTGTTGATGGAATTTTGGTGTTATCAGGGCGATCAACCCACAAAAGTG
CGGTGGATTTTAGACGTGGATCCGTTGGATCTGAGTTAG

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(505-588)

Primosomal protein N C-terminal domain (PriA_C)

(632-728)

DEAD/DEAH box helicase (DEAD)

(221-365)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

3DNA-binding domain (3BD) (PriA_3primeBD)

(5-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

42.51

100

0.43

Multiple sequence alignment