Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   SD89_RS00675 Genome accession   NZ_CP010449
Coordinates   104770..105204 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NGAS322     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99770..110204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD89_RS00645 (SD89_00645) - 101439..101804 (+) 366 WP_002986560.1 DUF1033 family protein -
  SD89_RS00650 (SD89_00650) comYA 101897..102835 (+) 939 WP_002993471.1 competence type IV pilus ATPase ComGA Machinery gene
  SD89_RS00655 (SD89_00655) comYB 102771..103805 (+) 1035 WP_227875237.1 competence type IV pilus assembly protein ComGB Machinery gene
  SD89_RS00660 (SD89_00660) comGC 103807..104133 (+) 327 WP_053308148.1 competence type IV pilus major pilin ComGC Machinery gene
  SD89_RS00665 (SD89_00665) comGD 104108..104536 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  SD89_RS00670 (SD89_00670) comGE 104493..104777 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  SD89_RS00675 (SD89_00675) comYF 104770..105204 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  SD89_RS00680 (SD89_00680) comGG 105188..105514 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  SD89_RS00685 (SD89_00685) comYH 105612..106565 (+) 954 WP_053308149.1 class I SAM-dependent methyltransferase Machinery gene
  SD89_RS00690 (SD89_00690) - 106624..107820 (+) 1197 WP_002987791.1 acetate kinase -
  SD89_RS00695 (SD89_00695) - 108006..108314 (+) 309 Protein_91 hypothetical protein -
  SD89_RS00700 (SD89_00700) proC 108397..109167 (-) 771 WP_011284426.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=137313 SD89_RS00675 WP_002992738.1 104770..105204(+) (comYF) [Streptococcus pyogenes strain NGAS322]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=137313 SD89_RS00675 WP_002992738.1 104770..105204(+) (comYF) [Streptococcus pyogenes strain NGAS322]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACTCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment