Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AOT11_RS08930 Genome accession   NZ_CP012881
Coordinates   1914366..1916090 (+) Length   574 a.a.
NCBI ID   WP_017420057.1    Uniprot ID   -
Organism   Vibrio vulnificus NBRC 15645 = ATCC 27562     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1909366..1921090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT11_RS08905 (AOT11_15940) gspM 1909770..1910420 (+) 651 WP_017420061.1 type II secretion system protein GspM -
  AOT11_RS08910 (AOT11_15945) - 1910413..1910733 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  AOT11_RS08915 (AOT11_15950) mshL 1910749..1912395 (+) 1647 WP_026050229.1 pilus (MSHA type) biogenesis protein MshL -
  AOT11_RS08920 (AOT11_15955) - 1912403..1913251 (+) 849 WP_017420059.1 ExeA family protein -
  AOT11_RS08925 (AOT11_15960) - 1913248..1914369 (+) 1122 WP_017420058.1 tetratricopeptide repeat protein -
  AOT11_RS08930 (AOT11_15965) pilF 1914366..1916090 (+) 1725 WP_017420057.1 GspE/PulE family protein Machinery gene
  AOT11_RS08935 (AOT11_15970) - 1916100..1917320 (+) 1221 WP_017420056.1 type II secretion system F family protein -
  AOT11_RS08940 (AOT11_15975) - 1917324..1917785 (+) 462 WP_017420055.1 hypothetical protein -
  AOT11_RS08945 (AOT11_15980) - 1917864..1918442 (+) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AOT11_RS24000 (AOT11_15985) - 1918476..1918922 (+) 447 WP_017420054.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AOT11_RS08955 (AOT11_15990) - 1918982..1919467 (+) 486 WP_017420053.1 type II secretion system protein -
  AOT11_RS08960 (AOT11_15995) - 1919467..1920033 (+) 567 WP_017420052.1 type IV pilus modification PilV family protein -
  AOT11_RS08965 (AOT11_16000) - 1920033..1920722 (+) 690 WP_017420051.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63686.06 Da        Isoelectric Point: 5.3246

>NTDB_id=137149 AOT11_RS08930 WP_017420057.1 1914366..1916090(+) (pilF) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLEEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=137149 AOT11_RS08930 WP_017420057.1 1914366..1916090(+) (pilF) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGATCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCACAAAGAAGTGGTGGGCGTAAGCTTGGCGATACGCTGATTGATTTAGGTTTTTTGTCTGAGCAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCTACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCATTGGTGATAGGCCGTAACGGCGACACGCTGCGCATTGCGATGAGTGA
TCCTGCCGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAGCTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCGGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGACGCATTTGATTTCAATATTGAAGATGACGACAGTGATGAGGTGACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGGGCATCGGATATCCATATCGAACCGGATGCCAATGTATTGCGTTTGCGCCAGC
GTATTGATGGCGTGCTACACGAAACCTTGCTAAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTAAAACTCATG
GCCAACTTGGATATTTCCGAAAAACGTCTGCCGCAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAATCGGTGGTGATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCAGGTTTGCCGGACGATCTTTTAGTGCGCCTACGCCGACAGTTGAAACGTCCGCACGGCATGATCCTCGTA
ACTGGCCCTACGGGTTCTGGTAAAACCACTACGCTGTATGGTGCTCTAACAGAACTCAACCAACCGGGCAAGAAGATCAT
CACGGCGGAAGATCCGGTCGAATATCGTTTGTCACGCGTCAACCAAGTGCAGATCAATCCAAAGATCAACCTTGATTTCT
CCACCGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTGGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCTGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACAGAAGATCACGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAGCGATTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCGCATGCTGCGCGTCGTTCTC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGAAGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.283

97.387

0.373
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371