Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AOT11_RS08540 Genome accession   NZ_CP012881
Coordinates   1824816..1827020 (-) Length   734 a.a.
NCBI ID   WP_026050203.1    Uniprot ID   -
Organism   Vibrio vulnificus NBRC 15645 = ATCC 27562     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1819816..1832020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT11_RS08520 (AOT11_15555) hslU 1820852..1822183 (-) 1332 WP_011079331.1 HslU--HslV peptidase ATPase subunit -
  AOT11_RS08525 (AOT11_15560) hslV 1822211..1822765 (-) 555 WP_017419639.1 ATP-dependent protease subunit HslV -
  AOT11_RS08530 (AOT11_15565) - 1822915..1823472 (-) 558 WP_017419640.1 SPOR domain-containing protein -
  AOT11_RS08535 (AOT11_15570) cytR 1823580..1824587 (-) 1008 WP_011079334.1 DNA-binding transcriptional regulator CytR Regulator
  AOT11_RS08540 (AOT11_15575) priA 1824816..1827020 (-) 2205 WP_026050203.1 primosomal protein N' Machinery gene
  AOT11_RS08545 (AOT11_15580) rpmE 1827301..1827522 (+) 222 WP_011079336.1 50S ribosomal protein L31 -
  AOT11_RS08550 (AOT11_15585) - 1827801..1829078 (+) 1278 WP_011079337.1 malic enzyme-like NAD(P)-binding protein -
  AOT11_RS08555 (AOT11_15590) metJ 1829164..1829481 (-) 318 WP_011079338.1 met regulon transcriptional regulator MetJ -
  AOT11_RS08560 (AOT11_15595) - 1829692..1830858 (+) 1167 WP_017419642.1 O-succinylhomoserine (thiol)-lyase -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82036.65 Da        Isoelectric Point: 8.5763

>NTDB_id=137140 AOT11_RS08540 WP_026050203.1 1824816..1827020(-) (priA) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
MRPSIARVALPVPLDKQFDYLVPGHLFPIIGGRVSVPFGRQTLIGIVTALVQESDFPREQLKPLKAVLDHRPIWPDSVYS
LLLWCSQFYQYPLGETLHNALPAALRKGKAADFATLQEWQLTELGKNQLMQGLNSRAIKQAKVLNMLANGPLPHQAFLDE
EVASTTLRTLEEKGWITCEEKTPQLTRWGNPIEHPVDKPKLNQEQAIAIATVNSQTDFGCYLLEGVTGSGKTEVYLNLIK
PILEQGKQALVLVPEIGLTPQTINRFRQRFDVPVAVIHSGLNDTERLNAWLSARDKAAGIVIGTRSALLIPFAQLGIIIV
DEEHDASYKQQDSLRYHARDVAVMRAAKEGIPIVLGSATPALETLHNALTGKYHHLSLTQRAGMAVPTTNKVLDVKGLYL
ESGLSAPLIAEMRRHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTYHQYSNEIRCHHCGSQQPIIHQCQGC
GSTQLVTVGVGTEQLENQLTTLFPDYKAIRIDRDSTRRKGSLESALEAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVILQTHHPEHTLLKALLTKDYRHFAQSALEERKIAQLPPFSFLTLF
RAEANHSEIVETFLRQVRMTLESHPLFDHSCLVLGPTPAPLAKRAGKYRWQLLLQTQHRSLMQKILSSTKPAIDLLPDAK
KVRWNLDIEPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=137140 AOT11_RS08540 WP_026050203.1 1824816..1827020(-) (priA) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
ATGCGTCCATCCATTGCTCGTGTTGCGCTGCCAGTGCCACTCGATAAACAATTTGATTATCTCGTTCCTGGACACCTTTT
TCCGATCATTGGTGGTCGCGTGAGTGTGCCGTTTGGACGACAAACCTTGATTGGCATTGTCACCGCTTTAGTTCAAGAAT
CAGACTTTCCACGCGAGCAACTTAAACCCCTTAAAGCAGTGTTGGATCATCGTCCTATTTGGCCCGATTCCGTCTACAGT
TTATTACTTTGGTGTAGCCAGTTTTACCAATATCCCCTAGGTGAAACACTGCATAACGCCCTCCCTGCTGCACTAAGAAA
AGGTAAAGCGGCTGACTTTGCGACTCTACAAGAATGGCAGTTGACCGAGTTAGGCAAAAACCAGTTGATGCAAGGGCTAA
ACAGTCGCGCGATAAAGCAAGCGAAAGTATTGAATATGCTTGCTAATGGCCCTCTGCCTCACCAAGCCTTTCTCGATGAG
GAAGTGGCATCCACAACCTTACGCACCTTGGAAGAAAAAGGCTGGATCACCTGTGAAGAAAAAACGCCACAGTTAACTCG
TTGGGGGAATCCGATTGAACATCCCGTCGATAAGCCGAAACTCAACCAAGAGCAAGCCATTGCCATTGCCACCGTAAACA
GCCAGACAGACTTTGGCTGTTATTTGTTGGAAGGAGTCACAGGCTCAGGCAAAACCGAAGTTTATCTCAACTTGATCAAA
CCCATTTTGGAACAAGGCAAACAGGCCCTGGTGTTGGTCCCTGAAATTGGTTTAACACCACAAACCATCAATCGTTTTCG
TCAACGGTTTGATGTGCCAGTCGCCGTCATCCATTCTGGTCTTAATGATACTGAACGTCTCAACGCTTGGCTTTCCGCAC
GAGACAAAGCGGCGGGCATTGTCATTGGTACACGCTCTGCACTACTGATCCCATTTGCCCAGCTAGGGATCATCATTGTC
GATGAAGAGCACGACGCTTCATACAAACAACAAGATAGCCTGCGTTACCATGCTCGCGATGTGGCTGTGATGCGAGCGGC
AAAAGAAGGCATTCCAATTGTTCTCGGTTCAGCCACGCCCGCGTTAGAAACCTTACACAACGCACTTACGGGCAAATATC
ATCATCTCTCGCTGACCCAACGAGCAGGAATGGCGGTGCCAACCACCAATAAAGTGCTGGATGTCAAAGGGCTCTATCTG
GAAAGTGGCCTCTCAGCGCCACTCATTGCTGAAATGCGTCGTCATTTAAAAGCGGGCAATCAGGTGATGCTATTTCTCAA
CCGTCGAGGCTTCTCCCCAGCGTTAATGTGCCATGAATGTGGTTGGATTGCCGAGTGCAAACGCTGCGATGCCTATTACA
CTTATCACCAGTACAGCAATGAAATCCGCTGTCACCACTGTGGCTCTCAACAACCTATTATTCACCAGTGCCAAGGTTGT
GGCTCTACACAACTGGTGACCGTTGGTGTCGGCACTGAGCAACTGGAAAACCAGTTGACGACCCTGTTTCCTGACTACAA
AGCAATTCGCATCGACCGCGATAGCACACGACGCAAAGGTAGCCTCGAAAGCGCTTTAGAAGCCATTCGCAAAGGGGAAT
ACCAAATTTTAATTGGCACGCAAATGCTGGCTAAAGGCCATCATTTCCCAGACGTGACGTTAGTGGCACTATTGGATGTT
GATGGGTCATTATACAGCAGTGACTTCCGAGCGTCCGAGCGATTGGCTCAGTTGTTTATTCAAGTCGCAGGACGAGCAGG
GCGTGCCAGCAAACCTGGCGAAGTGATCTTACAAACCCACCATCCAGAACACACTTTGCTCAAAGCTCTGTTGACCAAGG
ATTACCGGCACTTTGCGCAGTCTGCACTGGAAGAACGGAAAATTGCCCAACTTCCCCCGTTTAGTTTTCTAACGCTATTT
CGCGCAGAAGCCAATCACAGTGAAATCGTCGAAACATTCTTGCGCCAAGTGCGTATGACTCTCGAGTCTCATCCCTTGTT
TGATCACAGTTGTCTCGTGCTCGGCCCAACACCCGCCCCACTGGCCAAACGTGCGGGCAAATACCGCTGGCAGCTTCTGC
TTCAAACTCAACATCGTTCCTTGATGCAGAAAATACTCTCGAGCACGAAACCTGCTATCGATTTGTTACCAGATGCAAAA
AAAGTACGTTGGAACTTGGATATTGAGCCGCAGGATCTGAGTTAG

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

DEAD/DEAH box helicase (DEAD)

(205-364)

Helicase conserved C-terminal domain (Helicase_C)

(506-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.284

100

0.435