Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   Achr_RS04940 Genome accession   NZ_CP010415
Coordinates   1082017..1083615 (-) Length   532 a.a.
NCBI ID   WP_052264008.1    Uniprot ID   -
Organism   Azotobacter chroococcum NCIMB 8003     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1072883..1107707 1082017..1083615 within 0


Gene organization within MGE regions


Location: 1072883..1107707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Achr_RS04900 (Achr_9740) - 1072883..1073275 (+) 393 WP_039802368.1 type IV pilin protein -
  Achr_RS04905 (Achr_9750) pilV 1073281..1073847 (+) 567 WP_227028817.1 type IV pilus modification protein PilV -
  Achr_RS04910 (Achr_9760) - 1073844..1075019 (+) 1176 WP_039802370.1 PilW family protein -
  Achr_RS04915 (Achr_9770) - 1075016..1075501 (+) 486 WP_039802372.1 pilus assembly PilX N-terminal domain-containing protein -
  Achr_RS04920 (Achr_9780) - 1075536..1078781 (+) 3246 WP_193388189.1 pilus assembly protein -
  Achr_RS04925 (Achr_9790) - 1078778..1079329 (+) 552 WP_082045367.1 GspH/FimT family pseudopilin -
  Achr_RS04930 (Achr_9800) thiO 1079424..1080569 (+) 1146 WP_052263866.1 glycine oxidase ThiO -
  Achr_RS04935 (Achr_9810) pilR 1080633..1081973 (-) 1341 WP_039802374.1 sigma-54-dependent transcriptional regulator Regulator
  Achr_RS04940 (Achr_9820) pilS 1082017..1083615 (-) 1599 WP_052264008.1 sensor histidine kinase Regulator
  Achr_RS04945 (Achr_9830) - 1083641..1083883 (-) 243 WP_039802376.1 PP0621 family protein -
  Achr_RS04950 (Achr_9840) - 1084112..1085746 (+) 1635 WP_169531404.1 NAD+ synthase -
  Achr_RS04955 (Achr_9850) - 1086940..1088142 (-) 1203 WP_039802380.1 ISL3 family transposase -
  Achr_RS04960 (Achr_9860) - 1088639..1089655 (+) 1017 WP_227028818.1 substrate-binding domain-containing protein -
  Achr_RS24995 - 1090391..1090741 (+) 351 WP_144411506.1 hypothetical protein -
  Achr_RS04970 (Achr_9870) - 1090966..1092159 (+) 1194 WP_039802387.1 IS4 family transposase -
  Achr_RS25000 - 1092249..1092950 (+) 702 WP_144411507.1 hypothetical protein -
  Achr_RS25005 (Achr_9880) - 1092995..1093615 (-) 621 WP_227028819.1 hypothetical protein -
  Achr_RS26250 (Achr_9890) - 1093685..1094356 (-) 672 WP_227028820.1 hypothetical protein -
  Achr_RS04985 - 1094361..1094705 (-) 345 WP_039802388.1 hypothetical protein -
  Achr_RS04990 (Achr_9900) - 1095028..1096749 (-) 1722 Protein_1014 DUF927 domain-containing protein -
  Achr_RS04995 (Achr_9910) - 1096823..1097908 (+) 1086 WP_039807303.1 IS5 family transposase -
  Achr_RS05000 - 1098073..1098963 (-) 891 WP_039802391.1 hypothetical protein -
  Achr_RS05005 (Achr_9920) - 1099061..1100266 (-) 1206 WP_039802393.1 tyrosine-type recombinase/integrase -
  Achr_RS05015 (Achr_9940) queA 1100638..1101687 (+) 1050 WP_039802395.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  Achr_RS05020 (Achr_9950) tgt 1101693..1102808 (+) 1116 WP_039802397.1 tRNA guanosine(34) transglycosylase Tgt -
  Achr_RS05025 (Achr_9960) yajC 1102849..1103184 (+) 336 WP_039802399.1 preprotein translocase subunit YajC -
  Achr_RS05030 (Achr_9970) secD 1103248..1105116 (+) 1869 WP_039802401.1 protein translocase subunit SecD -
  Achr_RS05035 (Achr_9980) secF 1105127..1106041 (+) 915 WP_039802403.1 protein translocase subunit SecF -
  Achr_RS05040 (Achr_9990) - 1106132..1106689 (+) 558 WP_039802405.1 glycine zipper 2TM domain-containing protein -
  Achr_RS05045 (Achr_10000) - 1106754..1107707 (-) 954 WP_039802408.1 IS1595 family transposase -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58710.97 Da        Isoelectric Point: 8.6973

>NTDB_id=136977 Achr_RS04940 WP_052264008.1 1082017..1083615(-) (pilS) [Azotobacter chroococcum NCIMB 8003]
MIGTPLDGPQGGNVLRLYHLYRLVIGLLLVLLISSNLDERLLQVAHPELFHHGSWFYLILNILVAVLVHHPKPLQIFSLA
LVDVILLSGLLYTAGGPSSGIGNLIIVSVAVANILLHGRIGLLIAAVAASGLVYMTFYLGLNRPAAASQYVHAGALGSLC
FASALLVQSLTRRLRRSESLAERRAAEVADLQALNVQILERMRTGILVLDPQQQVLLANQSAFTLLGNPALTGQPLAAHC
PELVERLQQWQQNPTLRPQDLHTLPHGPLVQPNFVALTRGGRRGILVFLEDISQIAQQAQQLKLASLGRLTAGIAHEIRN
PLGAISHAAQLLQESECLDGPDRRLAQIIQDQSRRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFAGEYRASAAPNQIL
HLEIRDGSLKTRMDANQLIQILTNLVQNGLRYSTQCHPQGQVWLRLFRAPESDLPVLEVRDDGPGVPAEQIEHIFEPFFT
TETKGTGLGLYISRELCESNQARLDYRGGAGEGGCFRITFAHPRKLTQARHG

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=136977 Achr_RS04940 WP_052264008.1 1082017..1083615(-) (pilS) [Azotobacter chroococcum NCIMB 8003]
CTGATCGGCACCCCGCTGGACGGCCCCCAGGGCGGCAACGTCCTGCGCCTGTACCACCTGTACCGGCTGGTCATCGGCCT
GCTGCTGGTGCTGCTGATCTCCAGCAATCTGGACGAGCGGCTGTTGCAGGTCGCCCACCCGGAGCTGTTCCACCACGGCA
GCTGGTTCTACCTGATCCTGAACATTCTGGTCGCCGTTCTGGTGCACCACCCCAAACCCCTGCAGATCTTCAGCCTGGCA
CTGGTCGACGTGATCCTGCTTTCGGGCCTGCTCTACACGGCCGGTGGCCCGTCCAGCGGCATCGGCAACCTGATCATCGT
CTCGGTGGCCGTCGCCAACATCCTCCTGCACGGACGGATCGGCCTGCTGATCGCCGCCGTGGCGGCCAGCGGCCTGGTCT
ACATGACCTTCTACCTGGGCCTCAACCGCCCTGCGGCCGCCAGCCAGTACGTGCATGCGGGAGCGCTGGGCTCGCTGTGC
TTCGCCAGCGCGCTGCTCGTGCAGAGCCTGACCCGCCGCCTGCGGCGCAGCGAATCCCTGGCCGAACGACGCGCCGCCGA
GGTCGCCGACCTACAGGCCCTGAATGTACAGATCCTGGAGCGCATGCGGACCGGCATCCTGGTGCTCGACCCGCAGCAGC
AGGTGCTGCTGGCCAATCAGAGCGCCTTCACCCTGCTGGGCAACCCCGCACTGACCGGACAGCCGCTCGCCGCCCACTGC
CCGGAACTGGTCGAGCGCCTGCAGCAGTGGCAACAGAACCCGACCCTGCGCCCGCAGGACCTGCACACCCTGCCCCACGG
TCCGCTGGTGCAACCGAACTTCGTCGCCCTCACGCGGGGCGGACGGCGGGGCATCCTGGTCTTCCTCGAGGACATCTCGC
AGATCGCCCAGCAGGCCCAGCAGCTCAAGCTCGCCTCGCTCGGCCGGCTGACCGCCGGCATCGCCCACGAGATCCGCAAT
CCGCTCGGCGCCATCAGCCACGCCGCCCAGCTGCTGCAGGAGTCCGAGTGCCTCGACGGCCCCGACCGGCGCCTGGCGCA
GATCATCCAGGACCAGTCGCGGCGCATGAACCTGGTGATCGAGAACGTGCTGCAGCTGTCGCGCCGGCGCCAGGCCGAAC
CGCAGCTGCTCGACCTGAAGTACTGGCTGCACCGCTTCGCCGGCGAATACCGCGCCTCGGCGGCGCCGAACCAGATCCTG
CACCTGGAGATCCGGGACGGCTCCCTGAAGACCCGCATGGACGCGAACCAGCTGATCCAGATCCTCACCAACCTGGTGCA
GAACGGCCTGCGCTACAGCACCCAGTGCCACCCGCAGGGACAGGTCTGGCTCAGGCTGTTCCGCGCCCCGGAAAGCGACC
TGCCGGTCCTCGAAGTGCGCGACGACGGCCCCGGCGTGCCCGCCGAGCAGATCGAGCACATCTTCGAACCCTTCTTCACC
ACCGAAACCAAGGGCACCGGGCTGGGCCTGTACATCTCCCGCGAGCTGTGCGAGAGCAACCAGGCCCGCCTCGACTACCG
CGGCGGCGCAGGTGAAGGCGGCTGCTTCCGCATCACCTTCGCCCACCCACGCAAGCTGACCCAGGCACGCCACGGATGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(414-520)

His Kinase A (phospho-acceptor) domain (HisKA)

(306-370)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

64.818

98.308

0.637

Multiple sequence alignment