Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   AO356_RS33440 Genome accession   NZ_CP012831
Coordinates   4941980..4942684 (+) Length   234 a.a.
NCBI ID   WP_371919209.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4936980..4947684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS22090 (AO356_22065) - 4938798..4939472 (-) 675 WP_030141322.1 NuoB/complex I 20 kDa subunit family protein -
  AO356_RS22095 (AO356_22070) - 4939483..4939896 (-) 414 WP_003203367.1 NADH-quinone oxidoreductase subunit A -
  AO356_RS33435 - 4940897..4941781 (+) 885 Protein_4416 methyl-accepting chemotaxis protein -
  AO356_RS33440 pilJ 4941980..4942684 (+) 705 WP_371919209.1 methyl-accepting chemotaxis protein Machinery gene
  AO356_RS22105 (AO356_22080) aceA 4942793..4944118 (-) 1326 WP_014337679.1 isocitrate lyase -
  AO356_RS22110 (AO356_22085) - 4944575..4945336 (-) 762 WP_060741550.1 secretin N-terminal domain-containing protein -
  AO356_RS22115 (AO356_22090) - 4945579..4946004 (-) 426 WP_060741551.1 GNAT family N-acetyltransferase -
  AO356_RS22120 (AO356_22095) - 4945997..4947163 (-) 1167 WP_060741552.1 cupin domain-containing protein -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 24417.23 Da        Isoelectric Point: 4.4902

>NTDB_id=136790 AO356_RS33440 WP_371919209.1 4941980..4942684(+) (pilJ) [Pseudomonas fluorescens strain FW300-N2C3]
MSATVHEVARNAEQACAVASEADAQARIGDQVVAQAIVQIERLAGEVGRSVDAMGELEQESARIGKIMDVIKAVAEQTNL
LALNAAIEAARAGDAGRGFAVVADEVRGLAQRTQHSTEEIESLVAGLQNGTRQVSSIMRNSRELTDSSVELAGKAGISLG
NITQAVSGIQAMNQQIAAAAVQQSSVAEDISRSILSVRDVSEQTASASEETAASSIELARLGNQLQQLVSHFKV

Nucleotide


Download         Length: 705 bp        

>NTDB_id=136790 AO356_RS33440 WP_371919209.1 4941980..4942684(+) (pilJ) [Pseudomonas fluorescens strain FW300-N2C3]
ATGTCGGCCACCGTGCATGAAGTCGCGCGTAATGCCGAACAGGCTTGCGCAGTCGCGTCAGAGGCCGATGCGCAGGCCCG
TATCGGCGACCAGGTGGTCGCCCAGGCCATCGTTCAGATCGAGCGCTTGGCCGGCGAAGTCGGGCGCTCCGTCGACGCCA
TGGGTGAGTTGGAACAAGAAAGCGCGCGCATCGGCAAGATCATGGACGTGATCAAGGCGGTGGCTGAGCAGACCAACTTG
CTGGCCCTTAACGCCGCCATCGAGGCCGCTCGCGCGGGTGACGCCGGGCGCGGTTTTGCCGTGGTCGCCGACGAAGTGCG
GGGCCTGGCCCAGCGTACCCAGCATTCCACCGAAGAGATCGAAAGCCTGGTGGCGGGCCTGCAGAACGGCACGCGCCAGG
TGTCGAGCATCATGCGAAACAGCCGCGAACTGACCGACAGCAGCGTGGAATTGGCCGGCAAGGCCGGCATCTCCCTGGGC
AACATCACCCAGGCGGTTTCCGGCATCCAGGCCATGAACCAGCAGATTGCCGCTGCTGCGGTGCAACAAAGCTCGGTGGC
CGAGGACATCAGCCGCAGTATTCTGAGCGTGCGCGATGTATCCGAACAGACCGCCTCGGCCAGCGAAGAAACCGCCGCTT
CCAGCATCGAGCTGGCGCGCCTGGGTAACCAGTTGCAGCAACTGGTCAGTCACTTCAAAGTGTAA

Domains

Predicted by InterProScan.

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(18-200)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

36.1

100

0.372

Multiple sequence alignment