Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AO356_RS13080 Genome accession   NZ_CP012831
Coordinates   2970055..2971839 (-) Length   594 a.a.
NCBI ID   WP_060740144.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2965055..2976839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS13055 (AO356_13035) gcvT 2966401..2967483 (+) 1083 WP_060740140.1 glycine cleavage system aminomethyltransferase GcvT -
  AO356_RS13060 (AO356_13040) gcvH 2967536..2967919 (+) 384 WP_060740141.1 glycine cleavage system protein GcvH -
  AO356_RS13065 (AO356_13045) - 2968100..2968417 (-) 318 WP_060740142.1 DUF2388 domain-containing protein -
  AO356_RS13070 (AO356_13050) - 2968486..2968935 (-) 450 WP_060740143.1 NfeD family protein -
  AO356_RS13075 (AO356_13055) - 2968945..2969865 (-) 921 WP_014340768.1 SPFH domain-containing protein -
  AO356_RS13080 (AO356_13060) pilB 2970055..2971839 (-) 1785 WP_060740144.1 GspE/PulE family protein Machinery gene
  AO356_RS13085 (AO356_13065) - 2971985..2972323 (-) 339 WP_060740145.1 hypothetical protein -
  AO356_RS13090 (AO356_13070) - 2972450..2972920 (+) 471 WP_060743113.1 Lrp/AsnC family transcriptional regulator -
  AO356_RS13095 (AO356_13075) - 2973052..2974419 (-) 1368 WP_053189063.1 inorganic triphosphatase -
  AO356_RS13100 (AO356_13080) argE 2974578..2975735 (+) 1158 WP_060740146.1 acetylornithine deacetylase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 66350.96 Da        Isoelectric Point: 6.8654

>NTDB_id=136736 AO356_RS13080 WP_060740144.1 2970055..2971839(-) (pilB) [Pseudomonas fluorescens strain FW300-N2C3]
MSTKHATQDRWLDLNDLLRQLVTQGFISQDSAETALNARRRHSANSQLHPLEFIANQHLDDLSRPGKRLDLENLTLWLSQ
QAGQPYMRIDPLKINVAAVTPLMSYAFAQRHKILAVAVDRDAVTVASAQPYVSSWEADLTHVLKLPIKRVVANPVDIQRL
SVEFYRLAKSVSGATTTDQQPSNLSNFEQLLNLGASDQEPDANDAHIVNIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHTVYQFPPQVTMAIVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGGEVELRLSTLPTAFGEKMVMRIFDPEVLLKD
FDQLGFSADDLRRWQDMTRQPNGIILVTGPTGSGKTTTLYTTLKKLATPEVNLCTIEDPIEMVEASFNQMQVQHNIDLTF
AAGVRALMRQDPDIIMIGEIRDLETAEMAIQAALTGHLVLSTLHTNDAPSAISRLLELGVPHYLIKATVLGVMAQRLVRT
LCPQCKAPLALDEDDWQTLTRPWQAPLPSNAQQAVGCLECRDTGYRGRAGVYEIMQLTDSIKALIHPDTDLLAVRRQAFK
EGMRSLRLSGAQKVAAGLTTVEEVLRVTPQSEQK

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=136736 AO356_RS13080 WP_060740144.1 2970055..2971839(-) (pilB) [Pseudomonas fluorescens strain FW300-N2C3]
ATGTCCACCAAACACGCCACTCAGGACCGCTGGCTGGACCTCAATGATCTGCTGCGCCAACTGGTCACCCAGGGCTTCAT
CAGCCAGGATTCAGCCGAAACCGCGCTCAACGCCCGCCGACGCCACAGCGCCAATAGCCAGCTACATCCGCTGGAGTTCA
TTGCCAACCAACACCTGGACGACCTCAGTCGACCCGGCAAGCGCCTGGACCTGGAGAACCTGACCCTGTGGTTGTCCCAG
CAGGCCGGCCAACCCTACATGCGCATCGACCCGCTGAAAATCAACGTAGCGGCCGTGACGCCCTTGATGTCCTATGCCTT
TGCCCAGCGCCACAAGATCCTCGCAGTAGCCGTCGATCGTGACGCCGTCACCGTTGCCAGCGCACAACCCTACGTCAGCA
GCTGGGAAGCCGACCTGACCCACGTGCTCAAGCTGCCGATCAAGCGGGTGGTCGCCAACCCGGTGGATATCCAACGCTTG
AGCGTGGAGTTCTATCGCCTGGCCAAATCGGTCAGCGGCGCCACCACGACCGACCAGCAACCGAGCAACCTGAGCAATTT
CGAACAGTTACTCAACCTGGGGGCCAGTGACCAGGAGCCGGACGCCAACGACGCTCACATCGTCAATATCGTCGACTGGT
TGTTCCAATACGCCTTCCAGCAGCGGGCCAGCGATATCCACATCGAACCGCGCCGCGAGCAGGGCACGGTACGGTTTCGC
ATCGACGGGGTGCTGCACACCGTCTATCAGTTTCCGCCGCAGGTCACGATGGCGATCGTCAGCCGCCTCAAGAGCCTGGG
GCGGATGAACGTCGCGGAAAAACGCAAACCCCAGGACGGCCGGGTCAAGACCAAGACCCCGGACGGCGGCGAAGTGGAAT
TGCGCCTGTCGACCTTGCCCACGGCGTTCGGCGAAAAAATGGTCATGCGGATCTTCGACCCGGAAGTGCTGCTCAAGGAT
TTCGACCAGCTCGGTTTCTCCGCCGACGACCTGCGCCGCTGGCAGGACATGACCCGCCAGCCCAATGGCATCATCCTGGT
GACCGGACCTACCGGTTCCGGCAAGACCACCACCCTCTACACCACGCTCAAGAAACTGGCGACGCCGGAAGTGAACCTCT
GCACCATCGAGGACCCGATTGAAATGGTCGAGGCATCCTTCAACCAGATGCAGGTCCAGCACAACATCGACCTGACCTTC
GCCGCCGGGGTACGGGCACTGATGCGACAAGACCCGGACATCATCATGATCGGTGAGATCCGTGACCTGGAAACCGCTGA
AATGGCGATCCAGGCGGCGCTGACCGGGCACCTGGTGCTGTCGACGCTGCACACCAACGACGCGCCGAGCGCCATCAGCC
GCCTGCTGGAGCTGGGTGTGCCCCATTACCTGATCAAGGCCACCGTGCTCGGGGTCATGGCCCAACGGCTGGTGCGCACC
CTGTGCCCGCAATGCAAGGCCCCGCTGGCCCTGGACGAAGACGACTGGCAGACCCTGACGCGGCCCTGGCAGGCACCGTT
GCCGAGCAATGCCCAACAGGCCGTCGGCTGCCTGGAATGCCGCGACACCGGTTACCGCGGCCGCGCCGGTGTCTACGAAA
TCATGCAACTGACCGACAGCATCAAGGCCTTGATCCACCCCGACACCGACCTGCTGGCGGTGCGCCGCCAGGCCTTCAAG
GAAGGCATGCGCAGCCTGAGGCTGTCGGGGGCGCAAAAGGTGGCGGCGGGCTTGACCACCGTCGAGGAAGTCCTGCGCGT
GACACCGCAGAGCGAACAGAAGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(21-168)

Type II/IV secretion system protein (T2SSE)

(216-480)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.555

98.316

0.389
  pilB Acinetobacter baumannii D1279779

37.372

98.653

0.369