Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AO356_RS09455 Genome accession   NZ_CP012831
Coordinates   2137889..2140108 (+) Length   739 a.a.
NCBI ID   WP_060739564.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2132889..2145108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS09440 (AO356_09425) - 2135097..2136365 (-) 1269 WP_053117649.1 malic enzyme-like NAD(P)-binding protein -
  AO356_RS09445 (AO356_09430) - 2136551..2137333 (-) 783 WP_060739563.1 thermonuclease family protein -
  AO356_RS09450 (AO356_09435) rpmE 2137375..2137599 (-) 225 WP_014336159.1 50S ribosomal protein L31 -
  AO356_RS09455 (AO356_09440) priA 2137889..2140108 (+) 2220 WP_060739564.1 primosomal protein N' Machinery gene
  AO356_RS09460 (AO356_09445) argS 2140371..2142107 (+) 1737 WP_060739565.1 arginine--tRNA ligase -
  AO356_RS09465 (AO356_09450) - 2142109..2142804 (+) 696 WP_003196959.1 SPOR domain-containing protein -
  AO356_RS09470 (AO356_09455) hslV 2143061..2143591 (+) 531 WP_003196958.1 ATP-dependent protease subunit HslV -
  AO356_RS09475 (AO356_09460) hslU 2143642..2144979 (+) 1338 WP_060739566.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81884.34 Da        Isoelectric Point: 8.6093

>NTDB_id=136716 AO356_RS09455 WP_060739564.1 2137889..2140108(+) (priA) [Pseudomonas fluorescens strain FW300-N2C3]
MPNAILRLALPSPLRRLFDYRAPAGVLRAQLQPGMRLRVPFGRREMIGILVEVTDHSEVPVDKLKPALALLDATPPLPAA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAEARQERFWSVAPGASLDDPRIARAPRQREALATLAQHPHGVAHQLL
SKLMLSKDSLDLLLAKDLVQVEIRRHAPGARHEHWLAQPELPLNAEQRAACEAIRAGFDSYHAFLLAGVTGSGKTEVYLQ
LIRQTLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLEAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCERCDARMTVHQRHGELRCHHCGHVERVPRHC
PQCGKVDLRPVGAGTERAEERLGILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEGFLDEACSEAERLLTEQSLTGIELLGPVPAPMERRAGRYRAQLLLQANARAPLHRLLSAWLLALEQ
MPSGRAVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=136716 AO356_RS09455 WP_060739564.1 2137889..2140108(+) (priA) [Pseudomonas fluorescens strain FW300-N2C3]
GTGCCCAACGCCATCCTGCGCCTCGCCCTGCCTTCACCCCTGCGCCGCCTGTTCGACTACCGCGCCCCCGCCGGGGTCCT
GCGCGCCCAGTTGCAGCCGGGCATGCGCCTGCGGGTGCCGTTCGGCCGGCGGGAAATGATCGGCATCCTGGTGGAGGTCA
CCGATCACAGCGAGGTCCCGGTCGACAAGCTCAAACCAGCCCTGGCCCTGCTCGATGCCACGCCGCCGCTGCCCGCCGCG
CTGTTCAAGTTGTGCCTGTGGACGTCCCAGTATTACCAGCACAGCCTCGGCGACACCCTGAGCTGGGCGCTGCCGGTGCT
GCTGCGCCAGGGCGAGCTGGCCGAGGCGCGCCAGGAGCGGTTCTGGTCGGTGGCCCCGGGGGCCTCGCTGGACGATCCGC
GCATCGCCCGCGCGCCGCGCCAGCGCGAGGCCTTGGCGACCCTCGCCCAACACCCCCATGGCGTGGCCCACCAACTGCTG
AGCAAACTGATGCTCAGCAAGGACAGCCTCGACCTGTTGCTGGCCAAGGACCTGGTCCAGGTGGAGATCCGCCGGCACGC
CCCCGGCGCCCGCCACGAACATTGGCTGGCCCAGCCGGAACTGCCGCTCAACGCCGAACAGCGCGCCGCTTGCGAAGCGA
TTCGCGCAGGTTTCGACAGTTATCACGCCTTTCTGCTGGCGGGCGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCCAGACCCTGGAGGCCGGCAAGCAGGCCCTGGTGCTGATTCCGGAAATCAACCTCGGCCCCCAGACCCTGGC
GCGTTTCGAGCAACGCTTCAATGCGCGGATCGCCCTGGTGCACTCGGCAGTCAACGACCGCGAACGCCTGGAAGCCTGGC
TCGCCGCCCGCGACGGCGAGGCCGATATCATCATCGGCACCCGCTCGGCCCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGCTCTTATAAACAGCAGGAAGGCCTGCGCTATCACGCCCGCGACCTGGCGCTGGT
ACGCGCCCGCCAGGAAAACATCCCGATCGTCCTCGGCTCGGCCACGCCCTCGCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTACGGCCTGCTGCGCCTCAACGAGCGGGCCGGCGGCGCCAAGCAACCGCGGTTCCTGCGCCTGGACGTGAAAAGT
CGTCCACTGGACAGCGGTATTTCCGGGCCGATGCAGCAAGCCATCGGCCAGACCCTCGCCGCCGGGCAACAGGTCCTGGT
GTTCCTCAATCGCCGCGGTTTTGCCCCGACCCTGCTGTGCCACGACTGCGGCTGGATGTCTGGCTGCGAACGCTGCGACG
CGCGGATGACCGTGCATCAACGCCATGGCGAACTGCGTTGCCACCATTGCGGCCACGTCGAACGCGTGCCAAGGCATTGC
CCGCAGTGCGGCAAAGTGGACTTGCGCCCGGTCGGCGCCGGCACCGAACGCGCCGAGGAACGGCTGGGCATCCTGTTTCC
GGACTACCCGGTCTTGCGGGTGGATCGCGACAGCACCTCGCGCAAGGACGCGATGAACCAGTTGTTCGCAACGATCCAGA
AGGGCCAGCCGTGCATATTGGTGGGCACGCAGATGCTCGCCAAGGGGCATCACTTCCCGCGGGTCACGCTGGTGTCGATC
CTCGATGCCGATGGTGGGCTGTTCTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAGCTGATCGTCCAGGTCGCCGG
CCGGGCCGGGCGGGCCGAGGAACCGGGCAAAGTGATTATCCAGACGCACCTGGCCGATCACCCGCTGTTGATCCAACTGA
CCGAGCAGGGTTACTTCGCCTTCGCCGAACAGGCTTTGAGTGAACGCCGCAGCGCCGGCCTGCCGCCCTTTTCCCACCTG
GCGCTGCTACGGGCCGAAGCCCACAAGCCCGGGCAGGCCGAAGGTTTCCTCGATGAGGCCTGCAGCGAGGCGGAGCGTTT
GCTGACAGAGCAGAGCCTCACCGGCATCGAACTCCTGGGGCCGGTGCCGGCACCGATGGAGCGCCGGGCCGGGCGCTATC
GCGCGCAGCTTTTATTGCAGGCCAACGCCCGGGCGCCGCTGCATCGGCTATTGAGTGCCTGGCTGCTGGCGCTGGAGCAG
ATGCCCAGCGGACGGGCGGTACGTTGGTCGCTGGATGTGGATCCGGTGGATTTGTATTGA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

DEAD/DEAH box helicase (DEAD)

(207-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

46.875

99.594

0.467