Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LI62_RS20630 Genome accession   NZ_CP010376
Coordinates   4285001..4286434 (+) Length   477 a.a.
NCBI ID   WP_023295498.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. steigerwaltii strain 34977     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4280001..4291434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LI62_RS20600 (LI62_22025) - 4280568..4281545 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  LI62_RS20605 (LI62_22030) kdsD 4281559..4282545 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  LI62_RS20610 (LI62_22035) kdsC 4282560..4283126 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LI62_RS20615 (LI62_22040) lptC 4283123..4283698 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  LI62_RS20620 (LI62_22045) lptA 4283667..4284221 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LI62_RS20625 (LI62_22050) lptB 4284228..4284953 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  LI62_RS20630 (LI62_22055) rpoN/rpoN1 4285001..4286434 (+) 1434 WP_023295498.1 RNA polymerase factor sigma-54 Machinery gene
  LI62_RS20635 (LI62_22060) hpf 4286457..4286744 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  LI62_RS20640 (LI62_22065) ptsN 4286827..4287318 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LI62_RS20645 (LI62_22070) rapZ 4287364..4288218 (+) 855 WP_003861870.1 RNase adapter RapZ -
  LI62_RS20650 (LI62_22075) npr 4288215..4288487 (+) 273 WP_040118154.1 PTS phosphocarrier protein NPr -
  LI62_RS20655 (LI62_22080) mtgA 4288527..4289252 (-) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LI62_RS20660 (LI62_22085) elbB 4289249..4289902 (-) 654 WP_032672055.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54098.98 Da        Isoelectric Point: 4.3549

>NTDB_id=136681 LI62_RS20630 WP_023295498.1 4285001..4286434(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. steigerwaltii strain 34977]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGNDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=136681 LI62_RS20630 WP_023295498.1 4285001..4286434(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. steigerwaltii strain 34977]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCAATGACGAGATTGAACTGGAAGAGATTGAAGCCGTCCTGAAACGCGTTCAGCGCTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCATGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAGGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment