Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AO353_RS12445 Genome accession   NZ_CP012830
Coordinates   2736713..2738932 (-) Length   739 a.a.
NCBI ID   WP_054595222.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2731713..2743932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS12425 (AO353_12425) hslU 2731789..2733129 (-) 1341 WP_054595218.1 ATP-dependent protease ATPase subunit HslU -
  AO353_RS12430 (AO353_12430) hslV 2733187..2733717 (-) 531 WP_054595219.1 ATP-dependent protease subunit HslV -
  AO353_RS12435 (AO353_12435) - 2734005..2734706 (-) 702 WP_054595220.1 SPOR domain-containing protein -
  AO353_RS12440 (AO353_12440) argS 2734708..2736444 (-) 1737 WP_054595221.1 arginine--tRNA ligase -
  AO353_RS12445 (AO353_12445) priA 2736713..2738932 (-) 2220 WP_054595222.1 primosomal protein N' Machinery gene
  AO353_RS12450 (AO353_12450) rpmE 2739166..2739381 (+) 216 WP_054595223.1 50S ribosomal protein L31 -
  AO353_RS12455 (AO353_12455) - 2739390..2740208 (+) 819 WP_054595224.1 thermonuclease family protein -
  AO353_RS12460 (AO353_12460) - 2740390..2741658 (+) 1269 WP_054595225.1 malic enzyme-like NAD(P)-binding protein -
  AO353_RS12465 (AO353_12465) - 2742665..2743054 (+) 390 WP_054593867.1 DUF6124 family protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82105.58 Da        Isoelectric Point: 8.5507

>NTDB_id=136629 AO353_RS12445 WP_054595222.1 2736713..2738932(-) (priA) [Pseudomonas fluorescens strain FW300-N2E3]
MPDAILRLALPSPLRRLFDYRAPAGVLRAHLHPGMRLRVPFGRREMIGILVEVTSHSEVPADKLKPALALLDATPPLPAS
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAEARQERFWSITPGAKLDDPRIARAPRQREALATLAQHPHGVAHQLL
SKLMLSKDSLDLLLAKELVQVDIRKHAPGERHEHWLAQPELPLNPEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLESWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECQRCDARMTVHQRSGELRCHHCGYVERVPRHC
PKCGKVDLRPVGAGTERAEERLGILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRSAGLPPFAHL
ALLRAEAHKPGQAEGFLDEACSEAERLLDEHNLTGIELLGPVPAPMERRAGRYRAQLLLQASARAPLHRLLSAWLLALEQ
MPSGRAVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=136629 AO353_RS12445 WP_054595222.1 2736713..2738932(-) (priA) [Pseudomonas fluorescens strain FW300-N2E3]
GTGCCCGACGCCATTTTGCGCCTCGCCCTGCCTTCGCCCCTGCGCCGCCTGTTCGACTACCGTGCCCCGGCGGGAGTCCT
GCGCGCACATTTGCACCCGGGCATGCGGTTGCGGGTGCCGTTCGGCCGCCGCGAGATGATCGGGATCCTGGTGGAAGTCA
CCAGCCACAGCGAAGTCCCGGCCGACAAACTCAAACCGGCACTGGCGCTGCTCGATGCCACTCCACCGCTACCGGCATCA
CTGTTCAAGTTGTGCCTGTGGACGTCCCAGTATTACCAGCACAGCCTCGGCGACACCTTGAGTTGGGCGCTGCCGGTACT
GTTACGCCAGGGTGAACTGGCCGAAGCGCGCCAGGAGCGCTTCTGGTCGATCACCCCGGGCGCGAAACTGGATGATCCGC
GTATCGCCCGCGCGCCACGTCAGCGCGAAGCCTTGGCAACGCTGGCCCAGCATCCGCATGGCGTTGCCCATCAGTTGCTG
AGCAAGTTGATGTTGAGCAAGGACAGCCTCGACCTGCTGCTGGCCAAAGAACTGGTGCAAGTGGATATCCGCAAACACGC
ACCCGGCGAACGTCACGAACACTGGCTGGCGCAACCGGAGCTGCCGCTGAACCCGGAGCAGCGCGCCGCGTATGAAGCGA
TCCGCGCAGGTTTCGACAGTTTTCATGCGTTCCTGCTGGCCGGGGTCACTGGCAGCGGCAAGACCGAAGTCTATTTGCAG
CTGATCCGCGAAACCCTTGAGGCCGGCAAGCAGGCGCTGGTGCTGATCCCCGAAATCAACCTCGGGCCGCAAACCCTGGC
GCGCTTCGAACAGCGCTTCAATGCGCGTATTGCCCTCGTGCATTCGGCCGTCAATGACCGCGAACGGCTGGAATCCTGGC
TCGCGGCCCGGGATGGCGAGGCCGACATTATTATCGGCACCCGCTCGGCGCTGTTCACACCGATGAAAAACCCCGGGCTG
ATCATCATCGATGAAGAGCACGACGGCTCTTATAAACAGCAGGAAGGTTTGCGCTATCACGCCCGCGATCTGGCATTGGT
CCGAGCACGACAGGAAAACATCCCGATTGTCCTTGGCTCGGCGACGCCGTCACTGGAAAGCCTGCACAACGCCTACACCG
GTCGTTACGGACTGCTGCGCCTGAACGAGCGCGCCGGCGGGGCGAAACAACCCCGTTTCCTGCGCCTGGACGTCAAAAGT
CGCCCGCTGGACAGCGGTATTTCCGGGCCGATGCAGCAGGCGATCGGCCAGACCCTCGCTGCCGGCCAGCAAGTCCTGGT
ATTTCTCAACCGTCGCGGCTTTGCGCCCACCCTGCTCTGTCACGATTGCGGCTGGATGTCGGAGTGTCAGCGCTGCGATG
CGCGAATGACCGTCCACCAGCGTTCCGGCGAGCTGCGTTGCCACCATTGCGGCTACGTCGAGCGTGTCCCTCGGCACTGC
CCGAAATGCGGCAAGGTCGATTTGCGCCCCGTGGGTGCCGGCACCGAACGCGCTGAAGAGCGTTTGGGCATTCTGTTCCC
GGACTACCCGGTATTGCGGGTCGACCGCGACAGCACCTCGCGTAAAGATGCGATGAATCAGCTGTTCGCGACCATTCAGA
AAGGTCAGCCGTGCATTCTGGTGGGCACGCAGATGCTCGCCAAAGGGCATCACTTTCCACGGGTGACGCTGGTGTCGATT
CTCGATGCCGACGGCGGACTGTTCTCCGGTGATTTCCGCGCCAGCGAACGCATGGCTCAGTTGATCGTGCAGGTGGCGGG
ACGTGCCGGTCGCGCCGAAGAGCCGGGCAAAGTGATCATCCAGACTCACCTGGCTGACCACCCGTTGTTGGTGCAACTGA
CCGAGCAAGGTTATTTCGCCTTTGCCGAGCAGGCCTTGAGCGAACGTCGTTCCGCCGGGCTACCGCCGTTCGCGCACCTG
GCGCTGTTGCGGGCCGAAGCGCACAAACCGGGGCAGGCCGAAGGTTTCCTCGATGAAGCCTGCAGCGAGGCGGAACGATT
GTTGGATGAACACAACCTGACTGGCATCGAATTGCTCGGGCCGGTGCCGGCGCCGATGGAGCGCCGGGCTGGACGCTATC
GTGCGCAATTATTGTTGCAAGCCTCGGCTCGGGCGCCACTGCATCGACTGCTGAGCGCCTGGTTGCTGGCGCTGGAACAG
ATGCCGAGCGGGCGGGCGGTGCGCTGGTCTTTGGATGTTGATCCGGTCGATTTGTATTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(206-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

46.837

100

0.471

Multiple sequence alignment