Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   RR32_RS15540 Genome accession   NZ_CP010368
Coordinates   3305932..3306708 (-) Length   258 a.a.
NCBI ID   WP_308646142.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain 6411     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3300932..3311708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR32_RS15520 (RR32_15525) - 3301997..3302695 (-) 699 WP_002053293.1 YfgM family protein -
  RR32_RS15525 (RR32_15530) hisS 3302724..3304016 (-) 1293 WP_032047499.1 histidine--tRNA ligase -
  RR32_RS15530 (RR32_15535) ispG 3304013..3305128 (-) 1116 WP_031982309.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RR32_RS15535 (RR32_15540) - 3305150..3305941 (-) 792 WP_002053491.1 helix-turn-helix domain-containing protein -
  RR32_RS15540 (RR32_15545) pilF 3305932..3306708 (-) 777 WP_308646142.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RR32_RS15545 (RR32_15550) rlmN 3306748..3307980 (-) 1233 WP_002053401.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  RR32_RS15550 (RR32_15555) ndk 3308103..3308534 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  RR32_RS15555 (RR32_15560) iscX 3308648..3308848 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  RR32_RS15560 (RR32_15565) - 3308948..3309499 (-) 552 WP_004710660.1 phosphatase PAP2 family protein -
  RR32_RS15565 (RR32_15570) - 3309524..3310823 (-) 1300 Protein_3072 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 258 a.a.        Molecular weight: 29154.25 Da        Isoelectric Point: 8.5456

>NTDB_id=136625 RR32_RS15540 WP_308646142.1 3305932..3306708(-) (pilF) [Acinetobacter nosocomialis strain 6411]
MLCMGVAVACLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGILLQQEGSKP
NLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDIASAEKTFK
QALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQLRALFPES
PEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 777 bp        

>NTDB_id=136625 RR32_RS15540 WP_308646142.1 3305932..3306708(-) (pilF) [Acinetobacter nosocomialis strain 6411]
ATGTTATGTATGGGGGTTGCTGTTGCATGTCTGGCCAGTGGTTGTCAGTCTTCGCCAACGCTTAAAAAGGACCCGGAAAA
AGCAGTTAAGGTGCGTACGCAGCTTGCCGCCGAATACATTCGTTCTGGTGACTTGGACTCTGCAAAGCGTTCCCTTGACC
AAGCTTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTATTGCAACAAGAAGGCAGTAAACCC
AATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAATGCTCAGGCGCGTAATAATTATGG
TACGTATTTGTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTGCTGGTGCAACATTAGGTTATGATC
AACGCTATCAAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATTGCGAGTGCTGAAAAAACATTCAAA
CAAGCACTCCTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAATTTTTTATTTGCAGCAGCAGATTCC
GGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGGGTGCTCGCGCACTTTGGATTGGTT
TAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAGTTACGTGCTTTATTCCCGGAAAGT
CCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(141-198)

Tetratricopeptide repeat (TPR_16)

(81-126)

Tetratricopeptide repeat (TPR_8)

(31-60)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.45

100

0.985
  pilF Acinetobacter baylyi ADP1

75

99.225

0.744

Multiple sequence alignment