Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EHS42_RS02620 Genome accession   NZ_CP010350
Coordinates   519172..519972 (+) Length   266 a.a.
NCBI ID   WP_010327532.1    Uniprot ID   -
Organism   Acinetobacter johnsonii XBB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514172..524972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHS42_RS02590 (RZ95_02580) - 514383..514799 (+) 417 WP_004983505.1 hypothetical protein -
  EHS42_RS02595 (RZ95_02585) - 514940..516220 (+) 1281 WP_010327534.1 glycosyltransferase family 4 protein -
  EHS42_RS02600 (RZ95_02590) - 516413..516964 (+) 552 WP_004697666.1 phosphatase PAP2 family protein -
  EHS42_RS02605 (RZ95_02595) iscX 517055..517255 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  EHS42_RS02610 (RZ95_02600) ndk 517366..517797 (+) 432 WP_004697669.1 nucleoside-diphosphate kinase -
  EHS42_RS02615 (RZ95_02605) rlmN 517920..519155 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EHS42_RS02620 (RZ95_02610) pilF 519172..519972 (+) 801 WP_010327532.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EHS42_RS02625 (RZ95_02615) - 519963..520739 (+) 777 WP_010327531.1 helix-turn-helix domain-containing protein -
  EHS42_RS02630 (RZ95_02620) ispG 520758..521879 (+) 1122 WP_010327530.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EHS42_RS02635 (RZ95_02625) hisS 521883..523178 (+) 1296 WP_010327529.1 histidine--tRNA ligase -
  EHS42_RS02640 (RZ95_02630) - 523206..523904 (+) 699 WP_005401704.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30049.02 Da        Isoelectric Point: 9.1263

>NTDB_id=136551 EHS42_RS02620 WP_010327532.1 519172..519972(+) (pilF) [Acinetobacter johnsonii XBB1]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARASHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=136551 EHS42_RS02620 WP_010327532.1 519172..519972(+) (pilF) [Acinetobacter johnsonii XBB1]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGACCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTTTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAGAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAGTCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.284

100

0.688
  pilF Acinetobacter baylyi ADP1

64.286

100

0.643

Multiple sequence alignment