Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BF24_RS17525 Genome accession   NZ_CP010322
Coordinates   3036807..3038183 (+) Length   458 a.a.
NCBI ID   WP_001085211.1    Uniprot ID   A0A6L8P2X8
Organism   Bacillus anthracis strain Canadian Bison isolate A0369     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3031807..3043183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF24_RS17505 (BF24_3233) ctsR 3032000..3032461 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  BF24_RS17510 (BF24_3234) - 3032635..3033183 (+) 549 WP_000128392.1 UvrB/UvrC motif-containing protein -
  BF24_RS17515 (BF24_3235) - 3033188..3034252 (+) 1065 WP_000050832.1 protein arginine kinase -
  BF24_RS17520 (BF24_3236) clpC 3034275..3036710 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BF24_RS17525 (BF24_3237) radA 3036807..3038183 (+) 1377 WP_001085211.1 DNA repair protein RadA Machinery gene
  BF24_RS17530 (BF24_3238) disA 3038187..3039260 (+) 1074 WP_000392168.1 DNA integrity scanning diadenylate cyclase DisA -
  BF24_RS17535 (BF24_3239) - 3039421..3040530 (+) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  BF24_RS17540 (BF24_3240) ispD 3040547..3041227 (+) 681 WP_000288292.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BF24_RS17545 (BF24_3241) ispF 3041342..3041818 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49890.50 Da        Isoelectric Point: 7.1421

>NTDB_id=136420 BF24_RS17525 WP_001085211.1 3036807..3038183(+) (radA) [Bacillus anthracis strain Canadian Bison isolate A0369]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=136420 BF24_RS17525 WP_001085211.1 3036807..3038183(+) (radA) [Bacillus anthracis strain Canadian Bison isolate A0369]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAA
CAGAAGTAACAAAACCAAGGCGCCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTCCAAGAATTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGATCCTTAGTACTTATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCGGATTCTTCATATGATGTACTATATATATCGGGTGAGGAATCAGCAAAACAGA
TTAAACTGCGTGCCGATCGTCTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
ACACACATTGAAGAGATGAACCCAGCCTTTGTTGTTATTGACTCTATTCAAACAATACATTTACCTGAGGTAACATCAGC
GCCGGGGAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGCTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCAGAAGTATTAAACCCTTCTGAGATTTTCCTTGAGGAAAGACCAGTGGGGGTTGCGG
GATCAACAGTGGTTGCTTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCTCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGACGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCTATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTCAGAGATAAATCTACAGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTCGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6L8P2X8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.163

100

0.753
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629

Multiple sequence alignment