Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GBS85147_RS00705 Genome accession   NZ_CP010319
Coordinates   113887..115251 (+) Length   454 a.a.
NCBI ID   WP_001085194.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain GBS85147     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 108887..120251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBS85147_RS00680 (GBS85147_0139) rpoE 109597..110172 (+) 576 WP_000418426.1 DNA-directed RNA polymerase subunit delta -
  GBS85147_RS00685 (GBS85147_0140) - 110445..112049 (+) 1605 WP_000170432.1 CTP synthase -
  GBS85147_RS00690 (GBS85147_0141) - 112158..113084 (+) 927 WP_000723853.1 alpha/beta hydrolase -
  GBS85147_RS00700 (GBS85147_0143) - 113279..113725 (+) 447 WP_000049182.1 dUTP diphosphatase -
  GBS85147_RS00705 (GBS85147_0144) radA 113887..115251 (+) 1365 WP_001085194.1 DNA repair protein RadA Machinery gene
  GBS85147_RS00710 (GBS85147_0145) - 115387..115884 (+) 498 WP_000214743.1 carbonic anhydrase -
  GBS85147_RS00715 (GBS85147_0146) - 116038..117357 (+) 1320 WP_000759710.1 FAD-containing oxidoreductase -
  GBS85147_RS00720 (GBS85147_0147) gltX 117568..119022 (+) 1455 WP_001284497.1 glutamate--tRNA ligase -
  GBS85147_RS00725 (GBS85147_0148) - 119062..120030 (-) 969 WP_000668262.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49576.87 Da        Isoelectric Point: 6.5148

>NTDB_id=136342 GBS85147_RS00705 WP_001085194.1 113887..115251(+) (radA) [Streptococcus agalactiae strain GBS85147]
MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKNARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGEIGLTGEIR
RVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDIIGVTTVSQVLKAVFST

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=136342 GBS85147_RS00705 WP_001085194.1 113887..115251(+) (radA) [Streptococcus agalactiae strain GBS85147]
GTGGCTAAGAAAAAATCAGTCTTCACCTGTCAAGAGTGTGGTTACCAATCACCTAAGTATTTGGGACGTTGTCCAAATTG
CTCAGCCTGGTCTTCATTTGTAGAAGAGGTTGAAGTTCAAGAAGTTAAAAATGCACGAGTTAGTTTGAACGGTGAAAAGT
CCCGACCGACGAAGTTAAAAGATGTTTCCTCAATTAACTATTCTAGAACTAAGACTGATATGGATGAATTTAACCGTGTT
CTAGGAGGTGGTGTTGTACCAGGAAGTTTGGTTCTGATTGGAGGAGATCCTGGGATAGGTAAATCTACTTTATTACTACA
AGTATCAACTCAGCTAGCTAATAAAGGTACAGTATTATATGTCTCAGGTGAGGAATCTGCAGAACAGATTAAGTTACGAA
GTGAGCGTCTAGGCGATATCGATAATGAATTTTACCTTTATGCCGAGACTAATATGCAAAGTATTCGGTCGGAGATTGAG
AAGATTAAACCAGATTTTCTTATTATTGATTCTATACAAACTATTATGAGTCCCGAAGTATCAAGTGTCCAAGGCTCTGT
TAGCCAAGTTCGCGAAGTGACCGCTGAGTTGATGCAGTTAGCTAAAACTAATAATATTGCTACTTTTATTGTAGGTCATG
TGACTAAGGAGGGAACATTAGCAGGCCCTCGAATGCTAGAACATATGGTGGATACGGTTCTTTATTTTGAAGGGGAGCGT
CATCACACGTTCCGTATTTTACGGGCAGTCAAAAACCGTTTTGGTTCGACCAATGAAATAGGTATCTTTGAAATGCAGTC
TGGTGGTCTAGTTGAAGTTTTGAATCCAAGTCAAGTTTTTCTTGAAGAACGTTTAGACGGTGCAACAGGATCTGCTATTG
TTGTTACTATGGAAGGAACAAGGCCTATTTTAGCAGAGGTTCAAGCTTTAGTGACTCCAACTGTTTTTGGAAATGCTAAG
CGCACAACCACAGGTCTTGATTTTAATCGTGTCAGCCTTATTATGGCAGTTCTAGAAAAACGTTGTGGTTTATTGCTTCA
AAATCAAGATGCCTATTTAAAATCAGCGGGTGGTGTTAAATTGGATGAACCAGCAATTGATTTAGCTGTTGCTGTAGCTA
TTGCTTCCAGTTACAAAGAAAAACCCACAAATCCACAAGAATCATTTATTGGAGAAATTGGACTCACTGGTGAGATTCGT
CGCGTGACACGTATTGAGCAACGTATTAATGAGGCTAGTAAATTGGGCTTTACTAAAATTTACGCACCAAAGAATTCTCT
AGCTGGGATTGAAATACCTAAAGGCATTGATATTATTGGTGTGACAACTGTTAGTCAGGTCTTAAAAGCAGTGTTTTCAA
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

87.859

99.78

0.877
  radA Streptococcus pneumoniae D39

87.859

99.78

0.877
  radA Streptococcus pneumoniae R6

87.859

99.78

0.877
  radA Streptococcus pneumoniae TIGR4

87.859

99.78

0.877
  radA Streptococcus mitis NCTC 12261

87.859

99.78

0.877
  radA Streptococcus mitis SK321

87.638

99.78

0.874
  radA Bacillus subtilis subsp. subtilis str. 168

62.693

99.78

0.626

Multiple sequence alignment