Detailed information    

experimental Experimentally validated

Overview

Name   atpA   Type   Unclear
Locus tag   HI_0481 Genome accession   L42023
Coordinates   502548..504089 (-) Length   513 a.a.
NCBI ID   AAC22139.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   require for natural transformation   
Unclear

Function

Insertions in atpA, a gene coding for the alpha subunit of the F1 cytoplasmic domain of the ATP synthase, reduce transformation frequencies about 20-fold and cause a significant reduction in expression of competence-regulatory genes, while the expression of constitutive competence genes is only minimally affected.


Genomic Context

Location: 497548..509089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0476 - 498245..498397 (+) 153 AAC22144.1 predicted coding region HI0476 -
  HI_0477 - 498427..499629 (+) 1203 AAC22135.1 tyrosine-specific transport protein (tyrP) -
  HI_0478 - 499815..500243 (-) 429 AAC22136.1 ATP synthase F1, subunit epsilon (atpC) -
  HI_0479 - 500273..501646 (-) 1374 AAC22137.1 ATP synthase F1, subunit beta (atpD) -
  HI_0480 - 501663..502532 (-) 870 AAC22138.1 ATP synthase F1, subunit gamma (atpG) -
  HI_0481 atpA 502548..504089 (-) 1542 AAC22139.1 ATP synthase F1, subunit alpha (atpA) Unclear
  HI_0482 - 504102..504635 (-) 534 AAC22140.1 ATP synthase F1, subunit delta (atpH) -
  HI_0483 - 504648..505118 (-) 471 AAC22141.1 ATP synthase F0, subunit b (atpF) -
  HI_0484 - 505168..505422 (-) 255 AAC22142.1 ATP synthase F0, subunit c (atpE) -
  HI_0485 atpB 505478..506266 (-) 789 AAC22143.1 ATP synthase F0, subunit a (atpB) Unclear
  HI_0485.1 - 506303..506677 (-) 375 AAC22145.1 predicted coding region HI0485.1 -
  HI_0486 - 506803..507414 (-) 612 AAC22146.1 glucose-inhibited division protein (gidB) -
  HI_0487 - 507554..507931 (-) 378 AAC22157.1 predicted coding region HI0487 -
  HI_0488 - 508047..508649 (+) 603 AAC22147.1 conserved hypothetical protein -

Sequence

Protein


Download         Length: 513 a.a.        Molecular weight: 55591.28 Da        Isoelectric Point: 5.2794

>NTDB_id=1363 HI_0481 AAC22139.1 502548..504089(-) (atpA) [Haemophilus influenzae Rd KW20]
MQLNSTEISELIKKRIAQFDVVSEARNTGTIVSVSDGIIRIHGLSDVMQGEMIALPGNRYAMALNLERDSVGAVVMGPYA
DLAEGMEVQCTGRILEVPVGRGLLGRVVNTLGQPIDGKGEIENDGFSPVEVIAPGVIDRRSVDQPVQTGYKAVDSMVPIG
RGQRELIIGDRQTGKTALAIDAIINQRNSGIKCIYVAIGQKASTIANVVRKLEEHGALANTIVVAASASESAALQYLAPY
AGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLLLRRPPGREAYPGDVFYLHSRLLERASRVNEDYVEKFTKGEVKGK
TGSLTALPIIETQAGDVSAFVPTNVISITDGQIFLESNLFNSGIRPAVNPGISVSRVGGSAQTKVIKKLAGGIRTALAQY
RELAAFAQFASDLDDATRKQLSHGEKVTELLKQKQFTPLSVAEQAVILFAVEFGYLDDVELSKIASFETALLDYSNRNHA
EFMQELNKTGNYNDEIKDTLKSILDGFKANSAW

Nucleotide


Download         Length: 1542 bp        

>NTDB_id=1363 HI_0481 AAC22139.1 502548..504089(-) (atpA) [Haemophilus influenzae Rd KW20]
ATGCAACTAAATTCAACTGAAATTAGTGAATTGATTAAAAAACGCATCGCTCAATTCGACGTGGTCAGTGAAGCGCGTAA
TACAGGGACAATTGTTTCTGTAAGCGATGGTATTATTCGCATCCACGGTTTAAGCGATGTGATGCAAGGTGAAATGATCG
CCTTACCAGGTAATCGTTATGCGATGGCACTTAACCTTGAACGCGATTCTGTTGGTGCTGTAGTTATGGGACCTTACGCA
GATTTAGCGGAAGGTATGGAAGTTCAATGTACAGGTCGTATTCTTGAAGTACCAGTTGGTCGTGGTTTATTAGGTCGTGT
AGTTAATACGCTTGGTCAGCCAATCGATGGTAAAGGCGAAATTGAAAATGATGGTTTCTCACCAGTAGAAGTGATTGCGC
CAGGTGTTATCGATCGTCGTTCTGTTGATCAACCTGTTCAAACTGGTTATAAAGCTGTCGATTCTATGGTACCGATTGGT
CGTGGTCAGCGTGAGTTAATCATCGGTGACCGTCAAACGGGTAAAACTGCGTTAGCAATTGACGCTATTATTAATCAACG
TAATTCAGGTATTAAATGTATCTATGTTGCGATTGGTCAAAAAGCCTCTACTATTGCAAATGTCGTGCGTAAATTAGAAG
AACATGGTGCGCTTGCAAATACTATCGTGGTAGCTGCATCTGCATCTGAATCAGCAGCGTTACAATATTTAGCCCCTTAT
GCTGGTTGTGCGATGGGGGAATATTTCCGTGATCGCGGCGAAGATGCGTTAATTGTTTATGATGATTTATCAAAACAAGC
CGTAGCTTATCGTCAAATTTCATTATTATTACGTCGTCCACCAGGTCGTGAAGCCTATCCAGGTGATGTGTTCTATTTAC
ATTCTCGTTTACTAGAACGTGCTTCTCGTGTAAATGAAGATTATGTAGAAAAATTCACTAAAGGCGAAGTAAAAGGAAAA
ACAGGTTCTTTAACCGCACTTCCGATTATTGAAACCCAAGCTGGTGACGTATCTGCGTTTGTTCCAACCAACGTAATTTC
TATTACCGATGGTCAGATCTTCTTAGAATCTAACTTGTTTAACTCAGGTATTCGTCCTGCGGTAAACCCAGGTATTTCGG
TATCTCGTGTTGGTGGTTCAGCGCAAACTAAAGTTATTAAGAAATTAGCGGGTGGTATTCGTACCGCGCTAGCTCAATAT
CGTGAATTAGCAGCGTTTGCACAGTTTGCATCAGATCTAGATGATGCGACTCGTAAGCAACTTTCTCACGGTGAAAAAGT
AACTGAATTATTAAAACAAAAACAATTCACGCCATTATCTGTTGCAGAACAAGCGGTTATATTGTTTGCTGTTGAATTTG
GTTATTTGGATGACGTGGAATTATCAAAAATTGCAAGTTTTGAGACCGCACTTTTAGATTATTCTAACCGTAATCACGCT
GAATTTATGCAAGAGCTTAATAAAACCGGTAACTATAATGATGAAATCAAAGATACTTTAAAAAGTATTTTAGATGGTTT
TAAAGCGAATAGTGCTTGGTAG

Domains

Predicted by InterproScan.

ATP synthase alpha/beta chain, C terminal domain (ATP-synt_ab_C)

(382-506)

ATP synthase alpha/beta family, nucleotide-binding domain (ATP-synt_ab)

(149-375)

ATP synthase alpha/beta family, beta-barrel domain (ATP-synt_ab_N)

(26-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] M L Gwinn et al. (1997) In vitro Tn7 mutagenesis of Haemophilus influenzae Rd and characterization of the role of atpA in transformation. Journal of Bacteriology 179(23):7315-20. [PMID: 9393695]