Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   RP72_RS13660 Genome accession   NZ_CP010314
Coordinates   2588439..2589206 (-) Length   255 a.a.
NCBI ID   WP_003230055.1    Uniprot ID   A0AAE2SJ05
Organism   Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2583439..2594206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP72_RS13640 (RP72_13640) ppsR 2583614..2584426 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  RP72_RS13645 (RP72_13645) ccpN 2584452..2585090 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  RP72_RS13650 (RP72_13650) glyS 2585223..2587262 (-) 2040 WP_003230059.1 glycine--tRNA ligase subunit beta -
  RP72_RS13655 (RP72_13655) glyQ 2587255..2588142 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  RP72_RS13660 (RP72_13660) recO 2588439..2589206 (-) 768 WP_003230055.1 DNA repair protein RecO Machinery gene
  RP72_RS22750 (RP72_13665) yqzL 2589243..2589386 (-) 144 WP_003230053.1 YqzL family protein -
  RP72_RS13670 (RP72_13670) era 2589534..2590439 (-) 906 WP_003230051.1 GTPase Era -
  RP72_RS13675 (RP72_13675) - 2590420..2590830 (-) 411 WP_003230049.1 cytidine deaminase -
  RP72_RS13680 (RP72_13680) unpK 2590949..2591320 (-) 372 WP_004398824.1 diacylglycerol kinase family protein -
  RP72_RS13685 (RP72_13685) ybeY 2591301..2591774 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  RP72_RS13690 (RP72_13690) pgpH 2591775..2593910 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29328.02 Da        Isoelectric Point: 8.3080

>NTDB_id=136285 RP72_RS13660 WP_003230055.1 2588439..2589206(-) (recO) [Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=136285 RP72_RS13660 WP_003230055.1 2588439..2589206(-) (recO) [Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)]
ATGCTGACAAAATGTGAAGGGATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATGTTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGTTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGGCTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment