Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FORC17_RS01890 Genome accession   NZ_CP012739
Coordinates   396670..398394 (+) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain FORC_017     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 391670..403394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC17_RS01865 (FORC17_0327) gspM 392074..392724 (+) 651 WP_045596391.1 type II secretion system protein GspM -
  FORC17_RS01870 (FORC17_0328) - 392717..393037 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  FORC17_RS01875 (FORC17_0329) mshL 393053..394699 (+) 1647 WP_145923139.1 pilus (MSHA type) biogenesis protein MshL -
  FORC17_RS01880 (FORC17_0330) - 394707..395555 (+) 849 WP_065090056.1 ExeA family protein -
  FORC17_RS01885 (FORC17_0331) - 395552..396673 (+) 1122 WP_065090057.1 tetratricopeptide repeat protein -
  FORC17_RS01890 (FORC17_0332) pilF 396670..398394 (+) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  FORC17_RS01895 (FORC17_0333) - 398404..399624 (+) 1221 WP_011079398.1 type II secretion system F family protein -
  FORC17_RS01900 (FORC17_0334) - 399628..400089 (+) 462 WP_039554781.1 hypothetical protein -
  FORC17_RS01905 (FORC17_0335) - 400168..400746 (+) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC17_RS01910 (FORC17_0336) - 400780..401250 (+) 471 WP_011151246.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC17_RS01915 (FORC17_0337) - 401302..401799 (+) 498 WP_011151245.1 type II secretion system protein -
  FORC17_RS01920 (FORC17_0338) - 401799..402365 (+) 567 WP_065090058.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC17_RS01925 (FORC17_0339) - 402365..403054 (+) 690 WP_065090059.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=136228 FORC17_RS01890 WP_011079397.1 396670..398394(+) (pilF) [Vibrio vulnificus strain FORC_017]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=136228 FORC17_RS01890 WP_011079397.1 396670..398394(+) (pilF) [Vibrio vulnificus strain FORC_017]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGGCGTAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCGCAGCAACTTGATCTGCCTTTGATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAGTTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTACAAGCGGAG
CACCAAGTCAACGACGCATTCGATTTCAATATCGAAGATGACGACAGCGATGAAGTGACGGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGCGCATCGGATATCCATATCGAACCGGACGCCAATGTGTTGCGCTTGCGCCAAC
GTATTGATGGTGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTAAAACTCATG
GCCAACTTGGATATTTCAGAAAAACGTCTGCCGCAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCATGGCATGATCCTTGTG
ACTGGCCCTACGGGCTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACAGACTCTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACGGTGTTAAGAACATTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGTAAGACAGAAGATCATGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAACGCTTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGCCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTTGAGTTTGCGCATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTATTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371

Multiple sequence alignment