Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RE92_RS20905 Genome accession   NZ_CP010268
Coordinates   4758921..4759358 (+) Length   145 a.a.
NCBI ID   WP_013371920.1    Uniprot ID   A0A3G8R818
Organism   Paenibacillus polymyxa strain Sb3-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4753921..4764358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE92_RS20880 (RE92_20880) - 4754711..4755379 (+) 669 WP_040103374.1 uracil-DNA glycosylase -
  RE92_RS20885 (RE92_20885) - 4755396..4755818 (+) 423 WP_040103375.1 hypothetical protein -
  RE92_RS20890 (RE92_20890) - 4755939..4757825 (+) 1887 WP_040103376.1 Ig-like domain-containing protein -
  RE92_RS20895 (RE92_20895) - 4757898..4758200 (-) 303 WP_013371923.1 hypothetical protein -
  RE92_RS20900 (RE92_20900) - 4758585..4758779 (-) 195 WP_013371921.1 hypothetical protein -
  RE92_RS20905 (RE92_20905) nucA/comI 4758921..4759358 (+) 438 WP_013371920.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RE92_RS26580 - 4759351..4759527 (-) 177 WP_013371919.1 hypothetical protein -
  RE92_RS20910 (RE92_20910) - 4759642..4761462 (+) 1821 WP_040103377.1 sensor histidine kinase -
  RE92_RS20915 (RE92_20915) - 4761437..4763062 (+) 1626 WP_040103378.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15964.19 Da        Isoelectric Point: 9.4243

>NTDB_id=135620 RE92_RS20905 WP_013371920.1 4758921..4759358(+) (nucA/comI) [Paenibacillus polymyxa strain Sb3-1]
MKKKLLSFIALVLLAVGAYWVEGDNLFTKITGENPPSSAQVTLQFPSGRYPETAQHIKEAIQAGKSPVCTIDREGAEQNR
KHSLAGVPTRKGFDRDEWPMAMCSEGGKGANVKYIAPKDNRGAGSWVSHQLDQYEDGTRVKFVIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=135620 RE92_RS20905 WP_013371920.1 4758921..4759358(+) (nucA/comI) [Paenibacillus polymyxa strain Sb3-1]
TTGAAGAAAAAGTTACTAAGTTTTATAGCCTTAGTGCTGCTAGCTGTGGGCGCGTACTGGGTTGAAGGAGACAATCTTTT
TACAAAAATAACAGGAGAAAACCCGCCTTCATCAGCTCAGGTAACGCTGCAATTTCCGTCAGGTCGTTATCCTGAAACGG
CGCAGCATATTAAGGAAGCCATTCAAGCGGGAAAATCACCAGTATGCACAATTGACCGGGAAGGAGCCGAGCAAAATCGT
AAGCATTCGCTTGCGGGTGTTCCTACTCGTAAAGGGTTTGATCGTGATGAATGGCCGATGGCTATGTGCTCAGAAGGGGG
CAAGGGTGCAAATGTCAAATACATAGCTCCTAAGGATAACCGTGGGGCAGGATCATGGGTCAGTCACCAGTTAGATCAGT
ATGAGGATGGAACTCGTGTAAAATTCGTTATTAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G8R818

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.308

71.724

0.483

Multiple sequence alignment