Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NF53_RS26310 Genome accession   NZ_CP010106
Coordinates   5110672..5112021 (-) Length   449 a.a.
NCBI ID   WP_000879425.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar indiana strain HD521     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5105672..5117021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF53_RS26275 (NF53_5098) - 5105717..5106412 (-) 696 WP_000466510.1 hypothetical protein -
  NF53_RS26280 (NF53_5099) - 5106399..5107187 (-) 789 WP_000120045.1 hypothetical protein -
  NF53_RS26285 (NF53_5100) - 5107215..5107784 (-) 570 WP_001211057.1 hypothetical protein -
  NF53_RS26290 (NF53_5101) - 5107902..5108375 (-) 474 WP_000511548.1 hypothetical protein -
  NF53_RS26295 (NF53_5102) raiA 5108780..5109322 (-) 543 WP_000671186.1 ribosome-associated translation inhibitor RaiA -
  NF53_RS26300 (NF53_5103) cspC 5109644..5109841 (-) 198 WP_001990088.1 cold shock protein CspC -
  NF53_RS26305 (NF53_5104) - 5109968..5110672 (-) 705 WP_002097937.1 ComF family protein -
  NF53_RS26310 (NF53_5105) comFA 5110672..5112021 (-) 1350 WP_000879425.1 ATP-dependent helicase ComFA Machinery gene
  NF53_RS26315 (NF53_5106) - 5112149..5113576 (-) 1428 WP_000499468.1 NlpC/P60 family protein -
  NF53_RS26320 (NF53_5107) - 5113725..5114039 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  NF53_RS26325 (NF53_5108) - 5114211..5115053 (-) 843 WP_002097936.1 DegV family protein -
  NF53_RS26330 (NF53_5109) - 5115290..5115925 (+) 636 WP_000926665.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51093.04 Da        Isoelectric Point: 9.9772

>NTDB_id=134249 NF53_RS26310 WP_000879425.1 5110672..5112021(-) (comFA) [Bacillus thuringiensis serovar indiana strain HD521]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNLNLLQWNGTLSTGQNLAAQGVIEAIRQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEVPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=134249 NF53_RS26310 WP_000879425.1 5110672..5112021(-) (comFA) [Bacillus thuringiensis serovar indiana strain HD521]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAACTTGTTACAGTGGAACGGTACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAGGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCAAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCGTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AACGAGGCGTAACGGTGAAAAATTTGCAAGTCGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGTCGAGCAGGGCGGAGCTTTGAAGTGCCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

DEAD/DEAH box helicase (DEAD)

(117-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.131

98.441

0.503
  comFA Latilactobacillus sakei subsp. sakei 23K

41.439

89.755

0.372

Multiple sequence alignment