Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NF53_RS22000 Genome accession   NZ_CP010106
Coordinates   4317853..4318599 (-) Length   248 a.a.
NCBI ID   WP_000487013.1    Uniprot ID   A0A9W5QDK4
Organism   Bacillus thuringiensis serovar indiana strain HD521     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4312853..4323599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF53_RS21980 (NF53_4268) dnaG 4313614..4315410 (-) 1797 WP_000528224.1 DNA primase -
  NF53_RS21990 (NF53_4270) - 4316271..4317083 (-) 813 WP_000368936.1 pyruvate, water dikinase regulatory protein -
  NF53_RS21995 (NF53_4271) - 4317113..4317745 (-) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  NF53_RS22000 (NF53_4272) recO 4317853..4318599 (-) 747 WP_000487013.1 DNA repair protein RecO Machinery gene
  NF53_RS22005 (NF53_4273) - 4318633..4318776 (-) 144 WP_000883924.1 YqzL family protein -
  NF53_RS22010 (NF53_4274) era 4318953..4319858 (-) 906 WP_001080229.1 GTPase Era -
  NF53_RS22015 (NF53_4275) - 4319851..4320249 (-) 399 WP_001086282.1 cytidine deaminase -
  NF53_RS22020 (NF53_4276) - 4320349..4320702 (-) 354 WP_000715469.1 diacylglycerol kinase family protein -
  NF53_RS22025 (NF53_4277) ybeY 4320699..4321169 (-) 471 WP_000054679.1 rRNA maturation RNase YbeY -
  NF53_RS22030 (NF53_4278) - 4321166..4323310 (-) 2145 WP_000091514.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28609.20 Da        Isoelectric Point: 8.0488

>NTDB_id=134239 NF53_RS22000 WP_000487013.1 4317853..4318599(-) (recO) [Bacillus thuringiensis serovar indiana strain HD521]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGQFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=134239 NF53_RS22000 WP_000487013.1 4317853..4318599(-) (recO) [Bacillus thuringiensis serovar indiana strain HD521]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCAAAAAAACCGAAAAGCCGATTAGCATCTGTGTCGCAATTAATGA
CACATGGCCAGTTTCTTATTCAAATGGGATCTGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGACATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTTAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCGTATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment