Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   NF53_RS05985 Genome accession   NZ_CP010106
Coordinates   1160197..1162023 (+) Length   608 a.a.
NCBI ID   WP_000003348.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar indiana strain HD521     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1155197..1167023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF53_RS05965 (NF53_1078) - 1155500..1156174 (-) 675 WP_000362615.1 TerC family protein -
  NF53_RS05970 (NF53_1079) mecA 1156520..1157203 (+) 684 WP_000350716.1 adaptor protein MecA Regulator
  NF53_RS05975 (NF53_1080) - 1157276..1158820 (+) 1545 WP_000799193.1 cardiolipin synthase -
  NF53_RS05980 (NF53_1081) - 1158901..1160145 (+) 1245 WP_000628327.1 competence protein CoiA -
  NF53_RS05985 (NF53_1082) pepF 1160197..1162023 (+) 1827 WP_000003348.1 oligoendopeptidase F Regulator
  NF53_RS33590 (NF53_1083) - 1162155..1162328 (-) 174 WP_000750355.1 hypothetical protein -
  NF53_RS05995 (NF53_1084) - 1162558..1163451 (-) 894 WP_000360656.1 ClpXP adapter SpxH family protein -
  NF53_RS06000 (NF53_1085) - 1163451..1163849 (-) 399 WP_000043386.1 hypothetical protein -
  NF53_RS06005 (NF53_1086) - 1164018..1164596 (-) 579 WP_000191123.1 CYTH domain-containing protein -
  NF53_RS06010 (NF53_1087) - 1164741..1165112 (+) 372 WP_001180004.1 hypothetical protein -
  NF53_RS06015 (NF53_1088) - 1165143..1165781 (+) 639 WP_001081490.1 GTP pyrophosphokinase family protein -
  NF53_RS06020 (NF53_1089) - 1165800..1166597 (+) 798 WP_000673182.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70199.57 Da        Isoelectric Point: 4.6443

>NTDB_id=134222 NF53_RS05985 WP_000003348.1 1160197..1162023(+) (pepF) [Bacillus thuringiensis serovar indiana strain HD521]
MSEQNKAKALPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSSSSNTFGMLNNADLKFPSIKGEDGEEIEITHGRYIQFLESDDPRVRKDAFKAVYETYGKFKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDLLLKSLNVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=134222 NF53_RS05985 WP_000003348.1 1160197..1162023(+) (pepF) [Bacillus thuringiensis serovar indiana strain HD521]
ATGTCTGAACAAAACAAAGCGAAAGCATTACCAGATCGCAACGAGATTGAAGAAGCAAGTACGTGGCGATTAGAAGATAT
TTTCCAAACGGATGCAGAATGGGAAAAAGAATTTCAAGCTATTAAAGAGCTATTGCCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGGACAATTTACTTGAGGCATTGCAATATGAAGATGAAATATCAATGCGATTAGGTAAGTTATAT
ACATATGCTCATATGCGTTACGATCAAGATACAACAAACTCTGTATATCAAGCATTAAATGATCGCGCAACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCGTATATTGTTCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTGA
AGGAAAATAGAGATTTAAGTGTATATGAACATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCAGAAGCT
GAGGAAGCTTTATTAGCGGAAGCGTCTGAAGTAATGAGTTCATCAAGCAATACATTCGGTATGTTGAATAACGCGGATTT
AAAATTCCCATCTATTAAAGGGGAAGACGGAGAAGAAATAGAAATTACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCCTCGTGTTCGCAAAGATGCATTTAAAGCTGTATATGAAACGTACGGAAAATTTAAAAACACATTCGCAAGTACG
TTAAGTGGAGCAGTAAAACGTAATAATTTCAATGCGCGTGTTCGTAAATATGATTCTGCTCGCCAAGCTGCATTGAGCAA
TAATAATATTCCGGAAGCAGTATACGATCAACTCGTTGAAACAGTAAATGACAACTTACATTTATTACACCGTTACATCG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTATGATTTATATACACCACTTGTACCAGAAGTGAAAATG
AATGTGAAATACGAAGAAGCGCAAGACCTTTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGACATTTTGAA
AGAGGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGGGCATATTCATCTGGTGCATATG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTACTATACAAGAAAAACACAACCGCACGTATATGGTGATTATTCCATCTTCGTAGCAGAAGTGGCATCTAC
TTGTAATGAAGCGCTTTTAAATGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGGTTCCGTGGTACTGTATTCCGTCAAACGATGTTTGCAGAGTTTGAACATATTATTCATAAGAAAGTACAA
GAAGGACATGCTGTCACGCCAGACATGCTAACGGAAATTTACTACGATTTAAATAAGAAATATTTCGGTGACGCTTTAGT
AATCGACGAAGAGATTGGTTTAGAATGGTCTCGTATTCCACACTTCTACTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACAGCTCTGTCTAAACAGATTTTAGAAGAAGGACAACCAGCAGTAGAACGTTATATTAACGAGTTC
TTAAAAGCAGGAAGCTCTGATTATCCAATTGAAGTGCTGAAAAAAGCAGGAGTAGATATGGCATCTCCTGAACCAGTAAA
AGAGGCGCTACAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.084

97.533

0.488

Multiple sequence alignment