Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   NF53_RS00020 Genome accession   NZ_CP010106
Coordinates   3301..4428 (+) Length   375 a.a.
NCBI ID   WP_000470745.1    Uniprot ID   A0A9W5QA17
Organism   Bacillus thuringiensis serovar indiana strain HD521     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF53_RS00005 (NF53_0001) dnaA 283..1623 (+) 1341 WP_000428017.1 chromosomal replication initiator protein DnaA -
  NF53_RS00010 (NF53_0002) dnaN 1803..2948 (+) 1146 WP_001212884.1 DNA polymerase III subunit beta -
  NF53_RS00015 (NF53_0003) yaaA 3076..3288 (+) 213 WP_000821364.1 S4 domain-containing protein YaaA -
  NF53_RS00020 (NF53_0004) recF 3301..4428 (+) 1128 WP_000470745.1 DNA replication/repair protein RecF Machinery gene
  NF53_RS00025 (NF53_0005) gyrB 4467..6389 (+) 1923 WP_000435982.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  NF53_RS00030 (NF53_0006) gyrA 6478..8949 (+) 2472 WP_001282864.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43362.67 Da        Isoelectric Point: 6.6001

>NTDB_id=134204 NF53_RS00020 WP_000470745.1 3301..4428(+) (recF) [Bacillus thuringiensis serovar indiana strain HD521]
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGAKILQKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRE

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=134204 NF53_RS00020 WP_000470745.1 3301..4428(+) (recF) [Bacillus thuringiensis serovar indiana strain HD521]
TTGTTTATTTCAGAAATACAATTAAAAAACTATCGCAATTATGAAAAATTAGAGCTTTCCTTTGAAGATAAGGTAAATGT
AATTATCGGCGAAAATGCACAAGGGAAAACAAATTTGATGGAAGCTATTTATGTTTTAGCGATGGCGAAATCTCATAGAA
CCTCTAATGATCGCGAACTTATCCGCTGGGATGAAGATTTTGGTCAAATAAAGGGGAAATTACAAAAAAGAAACAGTTCT
TTGTCTTTGGAATTAAATATTTCGAAAAAAGGTAAAAAGGCAAAATTAAATCAACTTGAACAACAAAAGTTAAGTCAATA
TATTGGCGTGATGAACGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAATTAGGACAAATAGCTCCTGTATATTTGTATGAATTAAGTCAATATCAAAAGGTGCTCACGCAACGA
AATCACTTGTTGAAAAAAATGCAAGGGAATAGTAAGAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTAATTGA
GCATGGTGCGAAAATACTGCAAAAACGTTTTGAATTTTTGCATTTGCTACAGGAATGGGCAGCTCCAATTCATCGAGGTA
TAAGCCGTGGATTAGAAGAGTTAGAAATTGTCTATAAACCAAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCTGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATTGGTCCTCATCG
TGACGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCGCAAGGACAACAACGAACGACCGCACTAT
CCCTAAAATTAGCTGAAATTGAATTGATTTATTCAGAGGTTAAAGAATATCCAATCCTTTTGTTGGATGATGTTTTATCA
GAATTAGATGATTATCGTCAATCACATCTTTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTTACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTAAAAGAAGCGAAAACAATTCATGTAACGAACGGCACTGTAGATTGTGAAATAGATA
GGGAATAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

66.576

98.133

0.653

Multiple sequence alignment