Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BG06_RS20555 Genome accession   NZ_CP010088
Coordinates   3827508..3827942 (+) Length   144 a.a.
NCBI ID   WP_000811510.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain 97-27     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3822508..3832942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG06_RS20535 (BG06_3969) - 3823398..3824561 (-) 1164 WP_000434569.1 aminotransferase A -
  BG06_RS20540 (BG06_3970) - 3824684..3824884 (-) 201 WP_000929281.1 hypothetical protein -
  BG06_RS20545 (BG06_3971) kinB 3825194..3826468 (+) 1275 WP_000420390.1 sporulation sensor histidine kinase KinB -
  BG06_RS20550 (BG06_3972) - 3826522..3827271 (-) 750 WP_000388464.1 DUF3967 domain-containing protein -
  BG06_RS20555 (BG06_3973) nucA/comI 3827508..3827942 (+) 435 WP_000811510.1 DNA-entry nuclease Machinery gene
  BG06_RS20560 (BG06_3974) comJ 3827979..3828368 (+) 390 WP_000424033.1 competence protein ComJ -
  BG06_RS20565 (BG06_3975) metE 3828405..3830693 (-) 2289 WP_001007629.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BG06_RS20575 (BG06_3976) - 3831620..3832177 (-) 558 WP_000062080.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16040.40 Da        Isoelectric Point: 8.4331

>NTDB_id=134135 BG06_RS20555 WP_000811510.1 3827508..3827942(+) (nucA/comI) [Bacillus thuringiensis strain 97-27]
MKQLKGIIISIIVILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=134135 BG06_RS20555 WP_000811510.1 3827508..3827942(+) (nucA/comI) [Bacillus thuringiensis strain 97-27]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGTAATTCTTTCTATTTTAGTAGCAGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment