Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   QR42_RS05160 Genome accession   NZ_CP010075
Coordinates   1057259..1060963 (+) Length   1234 a.a.
NCBI ID   WP_039177651.1    Uniprot ID   -
Organism   Bacillus sp. WP8     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1052259..1065963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR42_RS05145 (QR42_05145) - 1052565..1052975 (+) 411 WP_039177642.1 DoxX family protein -
  QR42_RS05150 (QR42_05150) - 1053173..1053619 (-) 447 WP_039177646.1 ferritin-like domain-containing protein -
  QR42_RS05155 (QR42_05155) addB 1053772..1057281 (+) 3510 WP_039177648.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QR42_RS05160 (QR42_05160) addA 1057259..1060963 (+) 3705 WP_039177651.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QR42_RS05165 (QR42_05165) sbcD 1060990..1062165 (+) 1176 WP_039177655.1 exonuclease subunit SbcD -
  QR42_RS05170 (QR42_05170) - 1062162..1065557 (+) 3396 WP_039177657.1 SMC family ATPase -
  QR42_RS05175 (QR42_05175) - 1065577..1065876 (+) 300 WP_039177660.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142312.46 Da        Isoelectric Point: 5.0124

>NTDB_id=134031 QR42_RS05160 WP_039177651.1 1057259..1060963(+) (addA) [Bacillus sp. WP8]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDVYKAGKPS
FFELVDRYTSDRHDLDLQWLVKKIYEFSRSHPSPEQWMRAFLSLYDVDVQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELVPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFEKAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEYAADGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTSDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMAMIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRSKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKEQQDEVVQALMEGLPVEEYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIALPSDEPYDDSRIQQLLHSLQQRDLLTNEQVQSIDQEGIAAFFSTSIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRFTGGLEAAVPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=134031 QR42_RS05160 WP_039177651.1 1057259..1060963(+) (addA) [Bacillus sp. WP8]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCGATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCAGCAGGGTCAGGTAAAACAGCAGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCAGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCGAAAAACCCCGGGTCTCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGTTTGCAGGTTCTGCGCACCTTTTATTATGAAATTGACCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTGGATGAACTGTTTGAGGATGTATATAAGGCTGGAAAGCCATCG
TTCTTTGAACTGGTTGATCGCTATACTTCTGACCGTCATGATTTAGACTTGCAATGGCTGGTGAAGAAAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTTCTTTCTCTTTATGATGTAGATGTTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGACCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTACTTGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGCGGCCCGGCACCGAGAGCAGAAAATTTTATAGATGATCTAGAGCAAGTCAATGAATT
AATCAGGCACCAAGATGATTTTGAGAAGCTTTACGAGCTGGTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAGCTTAAA
GATGAATACTTTATGCGCAGTCCAGCCCAGCATTTGAAGAGTTTAGCTGAAATGAAGCCGGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAAAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGATGCAGAAGGAAATTTGATCGAAACAGAAGCGGCTAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAAGATACAAACCTTGTACAAGAAACGATTTTAAAACTTGTATCTAAAGGGGA
ATATGCTGCAGACGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTACATCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGA
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGTAAAGATACAACGACAGAAATGCTTCTTGTCCATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAACTAGAGACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGATAT
TGTGATTTTGCTTCGTTCTATGCCATGGGCCCCGCAAATGATGGAGGAATTGAAGAAACAGGGAATCCCTGTGTATGCGA
ATCTTTCTTCCGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTCTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCACTTGTCCATTTAGACGAAAATGAAATGGCAATGATTCGCACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGGTTTTTCAGATTGCTGCGTAAATGGCGTGATTTCTCGATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAATCTCCGCGCTCTTTATGA
TCGATCAAAGCAGTATGAAAAAGCTGCCTTTAGAGGACTGTTCCGTTTTCTTCGTTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAAGATGTTGTTCGCATGATGACAATTCACAGCAGTAAAGGG
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAGCTAGGTTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTAGCGATGA
AAAAAAAGATGAGGAAAGAGCTGTTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCAGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAAGATTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTTTCGCATCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGGAGCAGCAAGACGAGGTGGTACAAGCTTTGATGGAGGGGCTGCCTGTTGAAGA
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCATATCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGCTCGT
GCCACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCACACAGT
CATGCAGCATATTGCGCTTCCTTCTGACGAGCCCTACGATGATTCACGTATACAGCAGCTGCTCCATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTCCAGTCAATTGATCAAGAAGGAATTGCAGCCTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCGAAAAGCGGATTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAGCTATATTTACTAG
ATTATAAAACCGATAGAGTGCAAGGAAGATTTACAGGCGGCCTTGAAGCAGCTGTGCCTATATTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
CGGGAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.182

100

0.665

Multiple sequence alignment