Detailed information NTG0133742

Bioinformatically predicted

Overview

• Name: sepM
• Type: Regulator
• Locus tag: QI18_RS11870
• Genome accession: NZ_CP010050
• Coordinates: 2373437..2374465 (-)
• Length: 342 a.a.
• NCBI ID: WP_003132657.1
• Uniprot ID: S6FVM6
• Organism: Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk
• Function: processing of CSP (predicted from homology) Competence regulation

Genomic Context

Location: 2368437..2379465

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QI18_RS11845 (QI18_11845)	-	2368722..2369219 (-)	498	WP_039116485.1	nitroreductase family protein	-
QI18_RS11850 (QI18_11850)	-	2369206..2369415 (-)	210	WP_003132661.1	hypothetical protein	-
QI18_RS11855 (QI18_11855)	-	2369412..2370137 (-)	726	WP_017865174.1	radical SAM protein	-
QI18_RS11860 (QI18_11860)	-	2370233..2372356 (-)	2124	WP_039116487.1	MFS transporter	-
QI18_RS11865 (QI18_11865)	-	2372459..2373316 (-)	858	WP_039116490.1	transcriptional regulator	-
QI18_RS11870 (QI18_11870)	sepM	2373437..2374465 (-)	1029	WP_003132657.1	SepM family pheromone-processing serine protease	Regulator
QI18_RS11875 (QI18_11875)	coaD	2374452..2374949 (-)	498	WP_010906348.1	pantetheine-phosphate adenylyltransferase	-
QI18_RS11880 (QI18_11880)	rsmD	2374997..2375536 (-)	540	WP_050558566.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
QI18_RS11885 (QI18_11885)	-	2375707..2376927 (-)	1221	WP_039116754.1	MDR family MFS transporter	-
QI18_RS11890 (QI18_11890)	lmrP	2377010..2378236 (-)	1227	WP_039116491.1	multidrug efflux MFS transporter LmrP	-

Sequence

Protein

• Length: 342 a.a.
• Molecular weight: 37349.96 Da
• Isoelectric Point: 9.9183

>NTDB_id=133742 QI18_RS11870 WP_003132657.1 2373437..2374465(-) (sepM) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
MKKNKKINPKLKWGISIGLIVVALLALFYPTSYYVEMPGTTEPLGKMVKVEGKKDEHKGDFFLTTVQIARANLATMIYSH
FNSFTSIYSEQEMTGGLNDAQFNRVNQFYMETAQNTAIYQAFKLANKPYELKYEGVYVLDIAKNSTFKNKLELADTITAV
NGQQFTSSADMIAYVSKQKVGDSVTIEYTRIDGTKHKSTGKYIKIANGKTGIGISLVDHTEVVTTPKVTVNAGSIGGPSA
GMMFTLEIYSQLTGKDLRNGREIAGTGTIEHDGSIGQIGGVDKKVATASKEGAKVFLVPDSGTKKESSNNYLGAKAAAKK
LKTKMKIVPVKTIQDALDYLEK

Nucleotide

• Length: 1029 bp

>NTDB_id=133742 QI18_RS11870 WP_003132657.1 2373437..2374465(-) (sepM) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
ATGAAAAAAAATAAAAAAATTAATCCCAAATTAAAATGGGGAATCTCGATTGGTCTAATTGTTGTTGCCCTTTTAGCTTT
ATTTTATCCAACGAGTTACTATGTGGAAATGCCCGGAACAACCGAGCCTTTAGGGAAAATGGTCAAAGTTGAAGGCAAAA
AAGATGAACATAAAGGTGATTTTTTCCTGACGACTGTCCAAATTGCACGAGCAAATCTTGCTACAATGATTTACAGCCAT
TTTAATAGTTTTACAAGCATTTACAGTGAGCAAGAAATGACTGGTGGACTTAACGACGCACAGTTCAACCGTGTCAACCA
GTTTTACATGGAAACGGCACAGAATACAGCGATTTATCAAGCTTTTAAATTAGCTAATAAACCTTATGAACTTAAATATG
AAGGCGTTTATGTCCTTGATATTGCCAAAAATTCGACCTTTAAAAATAAGCTAGAACTTGCAGATACGATTACAGCAGTC
AATGGTCAACAGTTTACATCAAGTGCCGACATGATTGCTTATGTTTCCAAACAAAAAGTCGGTGATTCAGTAACGATTGA
ATACACGAGAATTGACGGCACAAAGCACAAGTCAACTGGCAAATACATCAAAATTGCTAATGGAAAAACAGGAATTGGGA
TCAGCCTAGTTGACCATACTGAAGTCGTGACGACTCCAAAAGTTACTGTCAACGCGGGTTCAATCGGCGGTCCCTCTGCG
GGGATGATGTTTACACTAGAAATTTACAGTCAGTTGACAGGAAAAGATTTGCGAAACGGTCGCGAAATTGCTGGAACTGG
AACGATTGAACATGACGGAAGCATTGGCCAAATCGGCGGTGTTGATAAAAAAGTAGCCACAGCGAGCAAAGAAGGGGCAA
AGGTCTTCTTAGTGCCTGACTCAGGCACCAAAAAGGAAAGTAGCAACAATTACCTTGGAGCCAAAGCCGCAGCTAAAAAA
CTAAAAACGAAGATGAAAATCGTTCCCGTCAAAACTATTCAGGACGCTTTAGATTATTTAGAAAAATAA

Domains

Predicted by InterproScan.

• PDZ domain (PDZ_2) (134-194)

• Lon protease (S16) C-terminal proteolytic domain (Lon_C) (227-338)

3D structure

• AlphaFold DB: S6FVM6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
sepM	Streptococcus mutans UA159	48.696	100	0.491