Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS04920   Type   Unclear
Locus tag   RA0C_RS04920 Genome accession   NC_017045
Coordinates   1018730..1020133 (+) Length   467 a.a.
NCBI ID   WP_004918215.1    Uniprot ID   M4T436
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

Among these genes, RA0C_RS04895, RA0C_RS05130, RA0C_RS09020, RA0C_RS02645, RA0C_RS04870, RA0C_RS05105, RA0C_RS04915, and RA0C_RS04920 were shown to be essential for natural transformation occurrence.


Genomic Context

Location: 1013730..1025133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS04910 (RA0C_1027) - 1014432..1015625 (-) 1194 WP_004918219.1 endonuclease/exonuclease/phosphatase family protein -
  RA0C_RS04915 (RA0C_1028) RA0C_RS04915 1015897..1018704 (+) 2808 WP_004918216.1 carboxypeptidase-like regulatory domain-containing protein Unclear
  RA0C_RS04920 (RA0C_1029) RA0C_RS04920 1018730..1020133 (+) 1404 WP_004918215.1 DUF5689 domain-containing protein Unclear
  RA0C_RS04930 (RA0C_1031) cas2 1021490..1021828 (-) 339 WP_004918211.1 CRISPR-associated endonuclease Cas2 -
  RA0C_RS04935 (RA0C_1032) cas1 1021818..1022717 (-) 900 WP_004918209.1 type II CRISPR-associated endonuclease Cas1 -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 51642.78 Da        Isoelectric Point: 5.1920

>NTDB_id=1335 RA0C_RS04920 WP_004918215.1 1018730..1020133(+) (RA0C_RS04920) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MKTTTYLKTSLIALSFGALTSCVHDDKFDTPAIQCSNQFSEPTTTIKDFKTLAPEPVNFVSNYTIPNGETDAPVIFEGYV
ISSDEGGNFYKQLTIQDNTENPTAGIQVAINKTFLYTDYPVGSRVRVRANGLSVGLDREVVKLGYVNPTGQIPEGNMRDF
ITGVCGGNTMDIKTITPRVVNSIAEATKDEYINTLVTIKGVQFSEADGKITYADAVNRQTVDRTLVDREDNTAVVRNSGF
ARFAGKTLPTKSGDITVVVNKYVSGRNVTWQLYIRDLNDVKFEQERFVSSKNVLGGNAPEYNKTIAETFDNVSTVTNFKR
IADPKYINYSVFGNRFWEQKTFNGNNYIQISAFRANADVSTYFIVPAEFTGSSKLSFQTKDGYYTGDALKVYYTTKYTPG
GVVNKTDLIDITSKFTLSKENTNGYAADWVDSGEFAIPATGKGYIFFEYAGNTTVTTTVQIDNITLK

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=1335 RA0C_RS04920 WP_004918215.1 1018730..1020133(+) (RA0C_RS04920) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGAAAACAACAACATATTTAAAAACTTCGTTAATAGCCCTCAGTTTTGGAGCATTAACCTCTTGCGTACACGACGATAA
GTTTGACACACCTGCAATCCAATGTAGCAACCAATTCTCGGAACCTACAACTACTATTAAAGATTTTAAAACATTGGCTC
CAGAACCTGTAAACTTCGTAAGCAACTACACTATCCCTAACGGAGAAACAGACGCTCCTGTTATTTTTGAAGGATATGTA
ATTTCTTCTGATGAAGGTGGTAACTTTTACAAACAACTTACCATACAAGACAATACCGAAAATCCTACCGCTGGTATCCA
AGTTGCCATAAACAAAACTTTCTTATATACAGACTATCCTGTGGGCTCTCGTGTAAGGGTTAGAGCTAACGGTCTTTCTG
TTGGCTTAGACAGAGAAGTAGTTAAACTAGGCTATGTAAATCCAACGGGACAAATACCTGAAGGTAATATGAGAGACTTC
ATCACTGGAGTTTGTGGAGGTAATACTATGGATATTAAAACCATTACTCCTAGAGTGGTAAACTCCATTGCAGAAGCTAC
TAAAGATGAATACATCAATACTCTAGTTACTATTAAAGGAGTACAATTTTCAGAGGCTGATGGTAAAATTACTTATGCAG
ATGCAGTCAATAGACAAACTGTTGATAGAACTTTAGTAGACAGAGAAGACAACACTGCTGTGGTGAGAAATAGTGGTTTT
GCTCGTTTTGCAGGGAAAACACTACCTACTAAAAGTGGAGACATCACCGTAGTTGTAAACAAATATGTTTCTGGAAGAAA
TGTAACATGGCAACTCTACATTCGTGATTTAAACGATGTTAAATTTGAACAAGAAAGATTTGTAAGTTCCAAAAATGTTT
TAGGTGGCAATGCTCCAGAATATAACAAGACCATAGCTGAAACTTTTGATAATGTATCTACAGTTACTAATTTCAAAAGA
ATTGCAGATCCTAAATACATTAACTATTCTGTATTTGGAAATCGTTTTTGGGAGCAAAAAACCTTTAATGGTAATAATTA
TATCCAAATAAGTGCATTTAGAGCCAATGCTGATGTTAGCACTTACTTTATAGTTCCTGCTGAGTTTACAGGCTCTTCTA
AGCTCTCTTTCCAAACAAAAGATGGTTATTACACAGGAGATGCTCTTAAAGTATATTACACTACGAAATATACACCTGGC
GGAGTTGTAAATAAAACCGACCTAATAGATATTACTTCTAAGTTTACTCTTTCAAAAGAAAATACTAATGGCTATGCTGC
CGATTGGGTAGATAGTGGCGAATTTGCAATTCCTGCTACAGGAAAAGGTTATATCTTTTTTGAATATGCAGGTAACACTA
CTGTAACTACTACTGTACAAATAGACAATATTACTTTAAAGTAG

Domains

Predicted by InterproScan.

Family of unknown function (DUF5689) (DUF5689)

(42-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4T436

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]