Detailed information NTG0001324

Experimentally validated

Overview

• Name: RA0C_RS04915
• Type: Unclear
• Locus tag: RA0C_RS04915
• Genome accession: NC_017045
• Coordinates: 1015897..1018704 (+)
• Length: 935 a.a.
• NCBI ID: WP_004918216.1
• Uniprot ID: E4TBQ7
• Organism: Riemerella anatipestifer ATCC 11845 = DSM 15868
• Function: require for natural transformation Unclear

Function

Among these genes, RA0C_RS04895, RA0C_RS05130, RA0C_RS09020, RA0C_RS02645, RA0C_RS04870, RA0C_RS05105, RA0C_RS04915, and RA0C_RS04920 were shown to be essential for natural transformation occurrence.

Genomic Context

Location: 1010897..1023704

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RA0C_RS04900 (RA0C_1025)	-	1012262..1012699 (-)	438	WP_004918223.1	hypothetical protein	-
RA0C_RS04905 (RA0C_1026)	-	1012699..1014327 (-)	1629	WP_004918222.1	acyl-CoA carboxylase subunit beta	-
RA0C_RS04910 (RA0C_1027)	-	1014432..1015625 (-)	1194	WP_004918219.1	endonuclease/exonuclease/phosphatase family protein	-
RA0C_RS04915 (RA0C_1028)	RA0C_RS04915	1015897..1018704 (+)	2808	WP_004918216.1	carboxypeptidase-like regulatory domain-containing protein	Unclear
RA0C_RS04920 (RA0C_1029)	RA0C_RS04920	1018730..1020133 (+)	1404	WP_004918215.1	DUF5689 domain-containing protein	Unclear
RA0C_RS04930 (RA0C_1031)	cas2	1021490..1021828 (-)	339	WP_004918211.1	CRISPR-associated endonuclease Cas2	-
RA0C_RS04935 (RA0C_1032)	cas1	1021818..1022717 (-)	900	WP_004918209.1	type II CRISPR-associated endonuclease Cas1	-

Sequence

Protein

• Length: 935 a.a.
• Molecular weight: 106919.36 Da
• Isoelectric Point: 9.0346

>NTDB_id=1324 RA0C_RS04915 WP_004918216.1 1015897..1018704(+) (RA0C_RS04915) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MIKKLSLVSMFCLLPASYYYAQTTVYAYVKDADGKPIENAVADLREANDDVKADKIGYFQFVDLKPGHYQMVVTQPNYET
KVLEFDVFENEKRKDLGIITLFSNLKNSELGVVLLDDNSSMDESSSQATLGLLQSSRDVFSNIAAFDLGFYWFRPRGIDG
RRSEVMMNGISMENADNGNVDYGNWGGLNEITRYPEIAVNHSPSEYAFGGVGSVVYKNMKASEYRKGFQFTQSLTNRNYR
NRTSLRYSSGMNKKGWAFTVMGARRWAQEGIQEGTFYDAYGAYLGIEKKVSDKHTLTLNLIGSPYRRSTASPNTQEVIDY
NGVHYNAFWGWQDGEKRSERVRKGFQPILQLTDYWKISSRSQLWATVSYQFGKDKASRLDWYNAQNPSPLYYRNLPSYFA
GLANPSDSDIAQLEITKNRWESNDQNYTQINWDRLYLANRNREQAVYFLVNDVNDDKVWNVGTHFVHNFSDKTQLFINAS
YRNYLSNQYREIKDLLGAQYALNGDPFASTNQPKLSGLFNEGEENTHKKVGDKIGYDYTFRRQETKINPALKFNTGNFDI
FVSALAAYITNNREGHFNHYLYKDALGKSENQDYWNFGLKGQVIYKLDGRNFLVYNSAYYSQAPYLNNLFINARVNSAIA
PNIKNTIVSANDISYMVSSPFVKVRATAFLINTQNDTNVQRFFADGIKLDGVDSEGNTTSARSAFVTQVMSNVNRRNMGA
ELGVEVKVTPTITVNGVANYGKYIYTNNPEVYFASDAVGMFPNEKLYTSYGTSYIKNFRQGGTPQEAYSLGVKYSSPKYW
WVGANWNYLGNNFLDPSPLIRSDFFVNNPVTGTPYAGISEEEYKRVTAQVKLPVAYFFNINAGKSWMLGKYYVLISATVN
NVLDNTKYATGGFEQTRNVNFADFRRDYDREYPNFAPRYFFTQGRNYFINLQVRF

Nucleotide

• Length: 2808 bp

>NTDB_id=1324 RA0C_RS04915 WP_004918216.1 1015897..1018704(+) (RA0C_RS04915) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGATTAAGAAATTATCATTAGTCTCTATGTTCTGTTTATTGCCTGCATCTTATTACTATGCACAAACCACAGTCTATGC
GTATGTGAAAGATGCCGATGGAAAACCCATTGAAAATGCAGTAGCCGACCTAAGAGAAGCCAATGACGATGTAAAGGCAG
ACAAAATTGGATATTTCCAATTTGTAGATTTAAAACCTGGACATTACCAAATGGTAGTAACTCAACCTAACTACGAAACC
AAAGTCCTAGAGTTTGATGTTTTTGAAAATGAAAAAAGAAAAGACTTGGGGATTATTACTCTTTTTTCTAACCTTAAAAA
TTCGGAATTAGGAGTAGTATTGCTAGATGACAATAGCAGTATGGACGAGTCTTCCTCACAAGCAACATTAGGACTATTAC
AGTCTTCTAGAGATGTGTTTAGTAATATTGCTGCCTTTGATTTAGGATTCTATTGGTTCAGACCAAGAGGTATAGATGGT
AGAAGAAGCGAGGTTATGATGAATGGTATCTCTATGGAAAATGCCGATAATGGCAATGTAGATTATGGCAACTGGGGAGG
ACTTAATGAAATCACAAGATACCCTGAAATTGCAGTAAATCATTCCCCTTCTGAGTACGCTTTCGGAGGCGTTGGCTCTG
TGGTCTACAAAAATATGAAAGCAAGTGAATACCGCAAAGGTTTCCAATTTACCCAATCTCTTACCAACAGAAACTATCGT
AACAGAACTTCCTTAAGATACTCTTCTGGAATGAATAAAAAGGGTTGGGCGTTTACTGTAATGGGAGCTAGAAGATGGGC
ACAAGAAGGGATACAAGAAGGTACTTTCTATGATGCTTACGGAGCTTACCTAGGAATTGAGAAAAAAGTAAGTGACAAAC
ATACACTTACCTTAAACTTAATAGGCTCTCCTTACCGTAGAAGTACTGCTAGCCCTAACACCCAAGAGGTAATAGATTAT
AATGGTGTACATTACAATGCTTTCTGGGGATGGCAAGATGGCGAGAAAAGAAGTGAAAGAGTAAGAAAAGGATTCCAACC
GATACTACAACTTACAGATTACTGGAAGATAAGCAGCAGATCTCAGCTTTGGGCTACTGTTTCTTACCAATTTGGTAAAG
ACAAGGCTTCTAGACTAGATTGGTATAACGCTCAGAACCCCTCTCCTCTATACTACAGAAATTTACCAAGCTATTTTGCT
GGATTAGCCAACCCTAGCGACTCCGATATAGCTCAACTGGAAATAACTAAAAACAGATGGGAAAGCAATGACCAAAACTA
CACCCAAATAAATTGGGATAGGCTTTACCTCGCTAATAGAAATAGAGAACAAGCGGTTTATTTCCTAGTAAATGATGTTA
ATGATGACAAAGTTTGGAATGTAGGAACTCATTTTGTTCATAATTTTTCTGACAAGACACAGCTATTCATCAACGCTTCT
TACAGAAATTATTTATCAAACCAATATAGAGAGATAAAAGACTTACTAGGAGCACAATACGCTCTTAATGGAGACCCTTT
TGCCTCTACCAACCAGCCTAAGCTTTCAGGTTTATTCAACGAAGGAGAAGAAAACACTCATAAAAAAGTAGGCGATAAAA
TAGGTTATGACTACACTTTCCGAAGACAAGAAACTAAGATAAACCCTGCTTTGAAATTTAACACAGGTAATTTTGATATT
TTCGTATCTGCCCTAGCGGCTTACATCACTAATAATAGAGAAGGGCATTTTAACCACTATCTTTACAAAGATGCATTAGG
AAAATCCGAAAACCAAGACTACTGGAATTTCGGCTTAAAAGGTCAGGTAATCTATAAATTAGATGGTAGAAATTTCTTAG
TTTATAATAGTGCCTACTATTCCCAAGCTCCTTACCTCAATAATTTGTTCATCAATGCAAGAGTAAACAGTGCCATCGCA
CCTAATATTAAAAACACCATAGTTTCGGCTAATGATATTAGCTATATGGTCTCTTCTCCATTTGTTAAAGTGAGAGCTAC
CGCATTCCTTATAAACACACAAAATGATACTAATGTACAGCGTTTCTTTGCTGATGGAATAAAACTAGATGGTGTAGATA
GCGAAGGCAACACCACTAGTGCGAGAAGTGCATTTGTAACACAAGTAATGTCTAATGTGAACCGTAGAAATATGGGGGCA
GAACTAGGTGTAGAGGTAAAAGTAACGCCTACTATTACCGTTAATGGGGTTGCCAATTATGGTAAATACATCTATACCAA
TAATCCTGAGGTGTACTTCGCTTCGGATGCGGTGGGAATGTTCCCGAACGAAAAACTTTACACCAGCTACGGAACTTCTT
ACATCAAAAACTTTAGACAAGGAGGCACACCACAAGAAGCCTACTCTCTAGGAGTAAAGTATAGTTCTCCTAAATACTGG
TGGGTAGGTGCTAACTGGAACTATCTTGGCAATAATTTCCTAGATCCATCTCCTCTCATTCGTTCTGACTTCTTTGTAAA
CAACCCAGTAACAGGAACACCGTATGCAGGAATTTCGGAGGAGGAATACAAAAGAGTTACCGCTCAAGTTAAACTCCCTG
TCGCTTATTTCTTTAACATCAATGCGGGTAAATCCTGGATGTTAGGCAAATATTATGTCCTAATTAGTGCAACTGTGAAC
AATGTGCTTGACAATACTAAATATGCTACTGGAGGCTTTGAGCAAACAAGAAATGTGAACTTTGCAGACTTTAGAAGAGA
CTACGACAGAGAGTATCCTAATTTTGCACCTAGATATTTCTTTACACAGGGTAGAAACTATTTTATCAATCTACAAGTAA
GATTCTAA

Domains

Predicted by InterproScan.

• Carboxypeptidase regulatory-like domain (CarboxypepD_reg) (25-87)

3D structure

• AlphaFold DB: E4TBQ7

References

• [1]: Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]