Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC8_RS01600 Genome accession   NZ_CP009982
Coordinates   332674..333690 (+) Length   338 a.a.
NCBI ID   WP_029802009.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_008     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327674..338690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC8_RS01585 (FORC8_0266) - 328098..328826 (-) 729 WP_015297397.1 redoxin family protein -
  FORC8_RS01590 (FORC8_0267) oxyR 328997..329905 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FORC8_RS01595 (FORC8_0268) - 329961..332531 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  FORC8_RS01600 (FORC8_0269) pilM 332674..333690 (+) 1017 WP_029802009.1 type IV pilus biogenesis protein PilM Machinery gene
  FORC8_RS01605 (FORC8_0270) pilN 333674..334252 (+) 579 WP_053046790.1 PilN domain-containing protein Machinery gene
  FORC8_RS01610 (FORC8_0271) pilO 334245..334838 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC8_RS01615 (FORC8_0272) pilP 334828..335343 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  FORC8_RS01620 (FORC8_0273) pilQ 335375..337123 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  FORC8_RS01625 (FORC8_0274) aroK 337312..337830 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37778.28 Da        Isoelectric Point: 6.8323

>NTDB_id=133401 FORC8_RS01600 WP_029802009.1 332674..333690(+) (pilM) [Vibrio parahaemolyticus strain FORC_008]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGSKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=133401 FORC8_RS01600 WP_029802009.1 332674..333690(+) (pilM) [Vibrio parahaemolyticus strain FORC_008]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGTCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.665

99.704

0.485

Multiple sequence alignment