Detailed information NTG0133392

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: BF25_RS25335
• Genome accession: NZ_CP009981
• Coordinates: 4487273..4488400 (-)
• Length: 375 a.a.
• NCBI ID: WP_000470753.1
• Uniprot ID: Q6I535
• Organism: Bacillus anthracis strain Ames isolate BACI008
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 4482273..4493400

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BF25_RS25325 (BF25_4814)	gyrA	4482752..4485223 (-)	2472	WP_001282851.1	DNA gyrase subunit A	-
BF25_RS25330 (BF25_4815)	gyrB	4485312..4487234 (-)	1923	WP_000435993.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-
BF25_RS25335 (BF25_4816)	recF	4487273..4488400 (-)	1128	WP_000470753.1	DNA replication/repair protein RecF	Machinery gene
BF25_RS25340 (BF25_4817)	yaaA	4488413..4488625 (-)	213	WP_000821367.1	S4 domain-containing protein YaaA	-
BF25_RS25345 (BF25_4818)	dnaN	4488753..4489892 (-)	1140	WP_001212525.1	DNA polymerase III subunit beta	-
BF25_RS25350 (BF25_4819)	dnaA	4490071..4491411 (-)	1341	WP_000428021.1	chromosomal replication initiator protein DnaA	-
BF25_RS25355 (BF25_4820)	rpmH	4491976..4492110 (+)	135	WP_000831901.1	50S ribosomal protein L34	-
BF25_RS25360 (BF25_4821)	rnpA	4492187..4492546 (+)	360	WP_000726628.1	ribonuclease P protein component	-
BF25_RS25365 (BF25_4822)	spoIIIJ	4492586..4493353 (+)	768	WP_000727748.1	YidC family membrane integrase SpoIIIJ	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 43362.71 Da
• Isoelectric Point: 6.9850

>NTDB_id=133392 BF25_RS25335 WP_000470753.1 4487273..4488400(-) (recF) [Bacillus anthracis strain Ames isolate BACI008]
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA

Nucleotide

• Length: 1128 bp

>NTDB_id=133392 BF25_RS25335 WP_000470753.1 4487273..4488400(-) (recF) [Bacillus anthracis strain Ames isolate BACI008]
TTGTTTATTTCAGAAATACAATTAAAAAACTATCGCAATTATGAAAAATTAGAGCTTTCCTTTGAAGATAAAGTAAATGT
AATTATCGGCGAGAACGCGCAAGGGAAAACGAATTTGATGGAAGCTATTTATGTTTTGGCGATGGCGAAATCTCATAGAA
CCTCTAATGATCGTGAGCTTATCCGTTGGGATGAAGATTTCGGTCAAATTAAAGGAAAGTTACAAAAGAGAAATAGTTCT
TTGTCTTTGGAATTAAATATCTCGAAAAAAGGTAAAAAGGCAAAATTAAATCAACTTGAACAACAAAAATTGAGTCAATA
TATTGGCGTGATGAATGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAATTAGGCCAAATAGCTCCGGTCTATTTGTATGAATTGAGTCAATATCAAAAGGTGCTCACACAACGA
AATCACTTGCTGAAAAAGATGCAAGGGAATAGTAAGAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTACAAAAATATTGCGAAAACGCTTTGAGTTTTTGCATTTACTACAAGAATGGGCAGCTCCAATTCATCGTGGTA
TAAGCCGTGGATTAGAAGAATTAGAAATTGTCTATAAACCGAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCAGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATTGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCGCAAGGACAACAACGAACGACCGCACTGT
CCCTAAAATTAGCTGAAATTGAATTGATTTACTCAGAAGTTAAGGAATATCCGATTCTTTTACTCGATGATGTATTATCA
GAATTAGATGATTATCGTCAATCGCATCTGTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTAAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATA
GGGCGTAA

Domains

Predicted by InterproScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (2-348)

3D structure

• AlphaFold DB: Q6I535

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Bacillus subtilis subsp. subtilis str. 168	66.304	98.133	0.651